A novel affinity-based method for the isolation of highly purified extracellular vesicles

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Nakai, Wataru ^a   Yoshida, Takeshi ^a,b   Diez, Diego ^a   Miyatake, Yuji ^a,b   Nishibu, Takahiro ^c   Imawaka, Naoko ^c   Naruse, Ken ^c   Sadamura, Yoshifusa ^c   Hanayama, Rikinari ^a,b,d  

^a OSAKA UNIVERSITY   (Japan)

^b KANAZAWA UNIVERSITY   (Japan)

^c FUJIFILM Wako Pure Chemical Corporation   (Japan)

^d JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84988813617     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep33935     Document Type: Article

References (32)

1. Trams, E. G., Lauter, C. J., Salem, N., Jr. & Heine, U. Exfoliation of membrane ecto-enzymes in the form of micro-vesicles. Biochim Biophys Acta 645, 63-70 (1981).
2. Colombo, M., Raposo, G. & Thery, C. Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu Rev Cell Dev Biol 30, 255-289 (2014).
3. Tkach, M. & Thery, C. Communication by Extracellular Vesicles: Where We Are and Where We Need to Go. Cell 164, 1226-1232 (2016).
4. Kramer-Albers, E. M. & Hill, A. F. Extracellular vesicles: interneural shuttles of complex messages. Curr Opin Neurobiol 39, 101-107 (2016).
5. Skog, J., et al. Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol 10, 1470-1476 (2008).
6. Baj-Krzyworzeka, M., et al. Tumour-derived microvesicles carry several surface determinants and mRNA of tumour cells and transfer some of these determinants to monocytes. Cancer Immunol Immunother 55, 808-818 (2006).
7. Valadi, H., et al. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 9, 654-659 (2007).
8. Raimondo, F., Morosi, L., Chinello, C., Magni, F. & Pitto, M. Advances in membranous vesicle and exosome proteomics improving biological understanding and biomarker discovery. Proteomics 11, 709-720 (2011).
9. Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci USA 113, E968-E977 (2016).
10. Thery, C., Amigorena, S., Raposo, G. & Clayton, A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol Chapter 3, Unit 3 22 (2006).
11. Balaj, L., et al. Heparin affinity purification of extracellular vesicles. Sci Rep 5, 10266 (2015).
12. Ghosh, A., et al. Rapid isolation of extracellular vesicles from cell culture and biological fluids using a synthetic peptide with specific affinity for heat shock proteins. PLoS One 9, e110443 (2014).
13. Stuart, M. C., et al. Ultrastructural detection of surface exposed phosphatidylserine on activated blood platelets. Thromb Haemost 74, 1145-1151 (1995).
14. Miyanishi, M., et al. Identification of Tim4 as a phosphatidylserine receptor. Nature 450, 435-439 (2007).
15. Thery, C., Ostrowski, M. & Segura, E. Membrane vesicles as conveyors of immune responses. Nat Rev Immunol 9, 581-593 (2009).
16. Feng, D., et al. Cellular internalization of exosomes occurs through phagocytosis. Traffic 11, 675-687 (2010).
17. Wei, X., et al. Surface Phosphatidylserine Is Responsible for the Internalization on Microvesicles Derived from Hypoxia-Induced Human Bone Marrow Mesenchymal Stem Cells into Human Endothelial Cells. PLoS One 11, e0147360 (2016).
18. Savina, A., Furlan, M., Vidal, M. & Colombo, M. I. Exosome release is regulated by a calcium-dependent mechanism in K562 cells. J Biol Chem 278, 20083-20090 (2003).
19. Hanayama, R., et al. Identification of a factor that links apoptotic cells to phagocytes. Nature 417, 182-187 (2002).
20. Veron, P., Segura, E., Sugano, G., Amigorena, S. & Thery, C. Accumulation of MFG-E8/lactadherin on exosomes from immature dendritic cells. Blood Cells Mol Dis 35, 81-88 (2005).
21. Hanayama, R. & Nagata, S. Impaired involution of mammary glands in the absence of milk fat globule EGF factor 8. Proc Natl Acad Sci USA 102, 16886-16891 (2005).
22. Dragovic, R. A., et al. Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis. Nanomedicine 7, 780-788 (2011).
23. Zhang, J., et al. Exosome and exosomal microRNA: trafficking, sorting, and function. Genomics Proteomics Bioinformatics 13, 17-24 (2015).
24. Raposo, G., et al. B lymphocytes secrete antigen-presenting vesicles. J Exp Med 183, 1161-1172 (1996).
25. Leventis, P. A. & Grinstein, S. The distribution and function of phosphatidylserine in cellular membranes. Annu Rev Biophys 39, 407-427 (2010).
26. Segawa, K., Kurata, S. & Nagata, S. Human Type IV P-type ATPases That Work as Plasma Membrane Phospholipid Flippases and Their Regulation by Caspase and Calcium. J Biol Chem 291, 762-772 (2016).
27. Nagata, S., Suzuki, J., Segawa, K. & Fujii, T. Exposure of phosphatidylserine on the cell surface. Cell Death Differ (2016).
28. Fadok, V. A., et al. Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J Immunol 148, 2207-2216 (1992).
29. Nagata, S., Hanayama, R. & Kawane, K. Autoimmunity and the clearance of dead cells. Cell 140, 619-630 (2010).
30. Gentleman, R. C., et al. Bioconductor: open software development for computational biology and bioinformatics. Genome Biol 5, R80 (2004).
31. Ritchie, M. E., et al. limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res 43, e47 (2015).
32. Hochberg, Y. & Benjamini, Y. More powerful procedures for multiple significance testing. Stat Med 9, 811-818 (1990).