Creating knockouts of conserved oligomeric golgi complex subunits using CRISPR-mediated gene editing paired with a selection strategy based on glycosylation defects associated with impaired COG complex function

Methods in Molecular Biology

Volumn 1496, Issue , 2016, Pages 145-161

  Blackburn, Jessica Bailey ^a   Lupashin, Vladimir V ^a  

^a NONE

Author keywords

Cholera toxin; COG; Conserved oligomeric Golgi complex; CRISPR; FAC sorting; Fluorescently tagged lectins; Glycosylation defects; Knockouts; SubAB toxin

Indexed keywords

COAT PROTEIN COMPLEX I; SNARE PROTEIN;

CELL SELECTION; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONSERVED SEQUENCE; CONTROLLED STUDY; CRISPR CAS SYSTEM; GENE INACTIVATION; GLYCOSYLATION; GOLGI COMPLEX; HOMEOSTASIS; INTRACELLULAR TRANSPORT; PHENOTYPE; PROTEIN SUBUNIT; GENE EDITING; GENETICS; HEK293 CELL LINE; HUMAN; METABOLISM; PROCEDURES;

CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; COAT PROTEIN COMPLEX I; GENE EDITING; GENE KNOCKOUT TECHNIQUES; GLYCOSYLATION; GOLGI APPARATUS; HEK293 CELLS; HUMANS; SNARE PROTEINS;

EID: 84987722769     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-6463-5_12     Document Type: Chapter

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