Different next generation sequencing platforms produce different microbial profiles and diversity in cystic fibrosis sputum

Journal of Microbiological Methods

Volumn 130, Issue , 2016, Pages 95-99

  Hahn, Andrea ^a,b,c   Sanyal, Amit ^d   Perez, Geovanny F ^a,c   Colberg Poley, Anamaris M ^b,c,d   Campos, Joseph ^a,c,d   Rose, Mary C ^b,c,d   Pérez Losada, Marcos ^b,d,e  

^a CHILDREN'S NATIONAL MEDICAL CENTER   (United States)

^b CHILDREN'S NATIONAL MEDICAL CENTER   (United States)

^c GEORGE WASHINGTON UNIVERSITY SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

^d GEORGE WASHINGTON UNIVERSITY   (United States)

^e CENTRO DE INVESTIGAÇÃO EM BIODIVERSIDADE E RECURSOS GENÉTICOS   (Portugal)

Author keywords

16S rRNA; Cystic fibrosis; Lung microbiome; MiSeq; Next generation sequencing; PacBio RSII

Indexed keywords

BACTERIAL DNA; RNA 16S;

AMPLICON; ARTICLE; BACTEROIDES; BURKHOLDERIA; COHORT ANALYSIS; CYSTIC FIBROSIS; ESCHERICHIA; GEMELLA; GENE LIBRARY; GENETIC ANALYZER; HAEMOPHILUS; HUMAN; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; MICROBIOME; NEXT GENERATION SEQUENCING; NONHUMAN; OPERATIONAL TAXONOMIC UNIT; PREVOTELLA; PRIORITY JOURNAL; PSEUDOMONAS; SHIGELLA; SPUTUM; STAPHYLOCOCCUS; STREPTOCOCCUS; TAXONOMIC IDENTIFICATION; TAXONOMY; BACTERIUM; BIODIVERSITY; BIOLOGY; CLASSIFICATION; GENETICS; HIGH THROUGHPUT SEQUENCING; LUNG; METAGENOME; MICROBIOLOGY; MICROFLORA; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PHYLOGENY; PROCEDURES;

BACTERIA; BASE SEQUENCE; BIODIVERSITY; CLASSIFICATION; COMPUTATIONAL BIOLOGY; CYSTIC FIBROSIS; DNA, BACTERIAL; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; LUNG; METAGENOME; MICROBIOTA; PHYLOGENY; RNA, RIBOSOMAL, 16S; SPUTUM;

EID: 84986879942     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2016.09.002     Document Type: Article

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