Implications of multiple freeze-thawing on respiratory samples for culture-independent analyses

Journal of Cystic Fibrosis

Volumn 14, Issue 4, 2015, Pages 464-467

  Cuthbertson, Leah ^a,b   Rogers, Geraint B ^c   Walker, Alan W ^d,e   Oliver, Anna ^a   Hoffman, Lucas R ^f,g   Carroll, Mary P ^h   Parkhill, Julian ^d   Bruce, Kenneth D ^b   van der Gast, Christopher J ^a  

^a CENTRE FOR ECOLOGY AND HYDROLOGY   (United Kingdom)

^b KING'S COLLEGE LONDON   (United Kingdom)

^c FLINDERS UNIVERSITY   (Australia)

^d WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^e ROWETT RESEARCH INSTITUTE   (United Kingdom)

^f UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^g UNIVERSITY OF WASHINGTON   (United States)

^h UNIVERSITY HOSPITAL SOUTHAMPTON NHS FOUNDATION TRUST   (United Kingdom)

Author keywords

Biobank; Microbiome; Microbiota; Propidium monoazide; Pyrosequencing; Sputum

Indexed keywords

DNA; PROPIDIUM IODIDE; RNA 16S;

ADULT; ARTICLE; BACTERIAL COLONIZATION; CLINICAL ARTICLE; CONTROLLED STUDY; CYSTIC FIBROSIS; DNA EXTRACTION; FREEZE THAWING; HUMAN; MICROBIAL COMMUNITY; MICROFLORA; NONHUMAN; PSEUDOMONAS AERUGINOSA; PYROSEQUENCING; RANDOMIZED CONTROLLED TRIAL; REPEAT PROCEDURE; SPUTUM ANALYSIS; SPUTUM CULTURE; STORAGE; BIOBANK; CRYOPRESERVATION; MICROBIOLOGY; PROCEDURES; SPECIMEN HANDLING; SPUTUM;

ADULT; BIOLOGICAL SPECIMEN BANKS; CRYOPRESERVATION; CYSTIC FIBROSIS; HUMANS; MICROBIOTA; SPECIMEN HANDLING; SPUTUM;

EID: 84937638103     PISSN: 15691993     EISSN: 18735010     Source Type: Journal    
DOI: 10.1016/j.jcf.2014.10.004     Document Type: Article

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