DMSO- and Serum-Free Cryopreservation of Wharton's Jelly Tissue Isolated From Human Umbilical Cord

Journal of Cellular Biochemistry

Volumn , Issue , 2016, Pages 2397-2412

  Shivakumar, Sharath Belame ^a   Bharti, Dinesh ^a   Subbarao, Raghavendra Baregundi ^a   Jang, Si Jung ^a   Park, Ji Sung ^a   Ullah, Imran ^a   Park, Ji Kwon ^b   Byun, June Ho ^b   Park, Bong Wook ^b   Rho, Gyu Jin ^a  

^a Gyeongsang National University   (South Korea)

^b Gyeongsang National University School of Medicine   (South Korea)

Author keywords

CELL SURVIVAL RATE; COCKTAIL SOLUTION; CRYOPROTECTANTS; FREEZING; WHARTON'S JELLY MESENCHYMAL STEM CELLS; WHARTON'S JELLY TISSUE

Indexed keywords

5' NUCLEOTIDASE; AGGRECAN; BONE MORPHOGENETIC PROTEIN 2; CELL SURFACE MARKER; COLLAGEN TYPE 2; CRYOPROTECTIVE AGENT; DIMETHYL SULFOXIDE; ENDOGLIN; FAT DROPLET; FATTY ACID BINDING PROTEIN 4; GLYCOSAMINOGLYCAN; LIPOCORTIN 5; LIPOPROTEIN LIPASE; OSTEONECTIN; PROTEIN BAX; PROTEIN BCL 2; PROTEIN P21; PROTEIN P53; PROTEOGLYCAN; THY 1 ANTIGEN; TRANSCRIPTION FACTOR RUNX2; TRANSCRIPTION FACTOR SOX9;

ADIPOCYTE; APOPTOSIS; ARTICLE; CARTILAGE CELL; CELL DIFFERENTIATION; CELL FUNCTION; CELL GROWTH; CELL LINEAGE; CELL STRUCTURE; CELL SURVIVAL; CONTROLLED STUDY; CRYOPRESERVATION; CRYOPROTECTION; FEMALE; FREEZING; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; LIVER CELL; MALE; MESENCHYMAL STEM CELL; OSTEOCYTE; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; SERUM-FREE MEDIUM; SURVIVAL RATE; THAWING; UMBILICAL CORD; WESTERN BLOTTING; WHARTON JELLY;

EID: 84984993604     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.25563     Document Type: Article

References (40)

1. Badowski M, Muise A, Harris DT. 2014. Mixed effects of long-term frozen storage on cord tissue stem cells. Cytotherapy 16:1313–1321.
2. Caplan AI, Dennis JE. 2006. Mesenchymal stem cells as trophic mediators. J Cell Biochem 98:1076–1084.
3. Carlin R, Davis D, Weiss M, Schultz B, Troyer D. 2006. Expression of early transcription factors Oct-4, Sox-2 and Nanog by porcine umbilical cord (PUC) matrix cells. Reprod Biol Endocrinol 4:8.
4. Castro-Malaspina H, Gay RE, Resnick G, Kapoor N, Meyers P, Chiarieri D, McKenzie S, Broxmeyer HE, Moore MA. 1980. Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. Blood 56:289–301.
5. Chao KC, Chao KF, Fu YS, Liu SH. 2008. Islet-like clusters derived from mesenchymal stem cells in Wharton's Jelly of the human umbilical cord for transplantation to control type 1 diabetes. PLoS ONE 3:e1451.
6. Chatzistamatiou TK, Papassavas AC, Michalopoulos E, Gamaloutsos C, Mallis P, Gontika I, Panagouli E, Koussoulakos SL, Stavropoulos-Giokas C. 2014. Optimizing isolation culture and freezing methods to preserve Wharton's jelly's mesenchymal stem cell (MSC) properties: An MSC banking protocol validation for the Hellenic Cord Blood Bank. Transfusion 54:3108–3120.
7. Choudhery MS, Badowski M, Muise A, Harris DT. 2013a. Utility of cryopreserved umbilical cord tissue for regenerative medicine. Curr Stem Cell Res Ther 8:370–380.
8. Choudhery MS, Badowski M, Muise A, Harris DT. 2013b. Comparison of human mesenchymal stem cells derived from adipose and cord tissue. Cytotherapy 15:330–343.
9. Choudhery MS, Khan M, Mahmood R, Mehmood A, Khan SN, Riazuddin S. 2012. Bone marrow derived mesenchymal stem cells from aged mice have reduced wound healing, angiogenesis, proliferation and anti-apoptosis capabilities. Cell Biol Int 36:747–753.
10. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop D, Horwitz E. 2006. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8:315–317.
11. Fong CY, Chak LL, Biswas A, Tan JH, Gauthaman K, Chan WK, Bongso A. 2011. Human Wharton's jelly stem cells have unique transcriptome profiles compared to human embryonic stem cells and other mesenchymal stem cells. Stem Cell Rev 7:1–16.
12. Fong CY, Subramanian A, Biswas A, Bongso A. 2016. Freezing of fresh Wharton's jelly from human umbilical cords yields high post-thaw mesenchymal stem cell numbers for cell-based therapies. J Cell Biochem 117:815–827.
13. Fong CY, Subramanian A, Biswas A, Gauthaman K, Srikanth P, Hande MP, Bongso A. 2010. Derivation efficiency, cell proliferation, freeze-thaw survival, stem-cell properties and differentiation of human Wharton's jelly stem cells. Reprod Biomed 21:391–401.
14. Gong W, Han Z, Zhao H, Wang Y, Wang J, Zhong J, Wang B, Wang S, Wang Y, Sun L, Han Z. 2012. Banking human umbilical cord-derived mesenchymal stromal cells for clinical use. Cell Transplant 21:207–216.
15. Gu TT, Liu SY, Zheng PS. 2012. Cytoplasmic NANOG-positive stromal cells promote human cervical cancer progression. Am J Pathol 181:652–661.
16. Heng BC. 2009. Effect of rho-associated kinase (ROCK) inhibitor Y-27632 on the post-thaw viability of cryopreserved human bone marrow-derived mesenchymal stem cells. Tissue Cell 41:376–380.
17. Hennerbichler S, Reichl B, Pleiner D, Gabriel C, Eibl J, Redl H. 2007. The influence of various storage conditions on cell viability in amniotic membrane. Cell Tissue Bank 8:1–8.
18. Jomha NM, Anoop PC, Bagnall K, McGann LE. 2004. Effects of increasing concentrations of dimethyl sulfoxide during cryopreservation of porcine articular cartilage. Cell Preserv Technol 1:111–120.
19. Le Blanc K. 2003. Immunomodulatory effects of fetal and adult mesenchymal stem cells. Cytotherapy 5:485–489.
20. Lee MS, Lill M, Makkar RR. 2004. Stem cell transplantation in myocardial infarction. Rev Cardiovasc Med 5:82–98.
21. Mason C, Manzotti E. 2010. Regenerative medicine cell therapies: Numbers of units manufactured and patients treated between 1988 and 2010. Regen Med 5:307–313.
22. Mazur P, Leibo SP, Chu EHY. 1972. A two-factor hypothesis of freezing injury. Exp Cell Res 71:345–355.
23. Morris G, Coulson G, Meyer M, Mclellan M, Fuller B, Grout B, Pritchard H, Knight S. 1983. Cold shock—A widespread cellualr reaction. Cryo Lett 4:179–192.
24. Morris GJ. 1987. Direct chilling injury. In: Grant BWW, Morris GJ, editors. The effects of low temperature on biological systems. London: Arnold Press. pp 120–146.
25. Park BW, Jang SJ, Byun JH, Kang YH, Choi MJ, Park WU, Lee WJ, Rho GJ. 2014. Cryopreservation of human dental follicle tissue for use as a resource of autologous mesenchymal stem cells. J Tissue Eng Regen Med DOI: 10.1002/term.1945
26. Parolini O, Alviano F, Bagnara GP, Bilic G, Bühring HJ, Evangelista M, Hennerbichler S, Liu B, Magatti M, Mao N, Miki T, Marongiu F, Nakajima H, Nikaido T, Portmann-Lanz CB, Sankar V, Soncini M, Stadler G, Surbek D, Takahashi TA, Redl H, Sakuragawa N, Wolbank S, Zeisberger S, Zisch A, Strom SC. 2008. Concise review: Isolation and characterization of cells from human term placenta: Outcome of the first international workshop on placenta derived stem cells. Stem Cells 26:300–311.
27. Patil R, Kumar BM, Lee WJ, Jeon RH, Jang SJ, Lee YM, Park BW, Byun JH, Ahn CS, Kim JW, Rho GJ. 2014. Multilineage potential and proteomic profiling of human dental stem cells derived from a single donor. Exp Cell Res 320:92–107.
28. Redaelli S, Bentivegna A, Foudah D, Miloso M, Redondo J, Riva G, Baronchelli S, Dalpra L, Tredici G. 2012. From cytogenomic to epigenomic profiles: Monitoring the biologic behavior of in vitro cultured human bone marrow mesenchymal stem cells. Stem Cell Res Ther 3:47.
29. Roy S, Arora S, Kumari P, Ta M. 2014. A simple and serum-free protocol for cryopreservation of human umbilical cord as source of Wharton's jelly mesenchymal stem cells. Cryobiology 68:467–472.
30. Ruiz-Delgado GJ, Mancias-Guerra C, Tamez-Gomez EL, Rodriguez-Romo LN, Lopez-Otero A, Hernandez-Arizpe A, Gomez-Almaguer D, Ruiz-Arguelles GJ. 2009. Dimethyl sulfoxide-induced toxicity in cord blood stem cell transplantation: Report of three cases and review of the literature. Acta Haematol 122:1–5.
31. Schmidt-Mende J, Hellstrom-Lindberg E, Joseph B, Zhivotovsky B. 2000. Freezing induces artificial cleavage of apoptosis-related proteins in human bone marrow cells. J Immunol Methods 245:91–94.
32. Shaw JM, Oranratnachai A, Trounson AO. 2000. Fundamental cryobiology of mammalian, oocytes and ovarian tissue. Theriogenology 53:59–72.
33. Shimazu T, Mori Y, Takahashi A, Tsunoda H, Tojo A, Nagamura-Inoue T. 2015. Serum- and xeno-free cryopreservation of human umbilical cord tissue as mesenchymal stromal cell source. Cytotherapy 17:593–600.
34. Singer NG, Caplan AI. 2011. Mesenchymal stem cells: Mechanisms of inflammation. Annu Rev Pathol 6:457–478.
35. Subramanian A, Fong CY, Biswas A, Bongso A. 2015. Comparative characterization of cells from the various compartments of the human umbilical cord shows that the Wharton's jelly compartment provides the best source of clinically utilizable mesenchymal stem cells. PLoS ONE 10:e0127992.
36. Sundin M, Ringden O, Sundberg B, Nava S, Gotherstrom C, Le Blanc K. 2007. No alloantibodies against mesenchymal stromal cells, but presence of anti-fetal calf serum antibodies, after transplantation in allogeneic hematopoietic stem cell recipients. Haematologica 92:1208–1215.
37. Takedachi M, Oohara H, Smith BJ, Iyama M, Kobashi M, Maeda K, Long CL, Humphrey MB, Stoecker BJ, Toyosawa S, Thompson LF, Murakami S. 2012. CD73-generated adenosine promotes osteoblast differentiation. J Cell Physiol 227:2622–2631.
38. Tse WT, Pendleton JD, Beyer WM, Egalka MC, Guinan EC. 2003. Suppression of allogeneic T-cell proliferation by human marrow stromal cells: Implications in transplantation. Transplantation 75:389–397.
39. Wiles MV, Taft RA. 2010. The sophisticated mouse: Protecting a precious reagent. Methods Mol Biol 602:23–36.
40. Yong KW, Pingguan-Murphy B, Xu F, Abas WABW, Choi JR, Omar SZ, Azmi MAN, Chua KH, Safwani WKZW. 2015. Phenotypic and functional characterization of long-term cryopreserved human adipose-derived stem cells. Sci Rep 5:9596.