Freezing induces artificial cleavage of apoptosis-related proteins in human bone marrow cells

Journal of Immunological Methods

Volumn 245, Issue 1-2, 2000, Pages 91-94

  Schmidt Mende, Jan ^a,b   Hellström Lindberg, Eva ^b   Joseph, Bertrand ^a   Zhivotovsky, Boris ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Apoptosis; Bone marrow; Cell storage; Immunoblot; MDS; P39

Indexed keywords

PROTEIN; PROTEINASE INHIBITOR;

APOPTOSIS; ARTICLE; BONE MARROW CELL; ENZYME ACTIVITY; FREEZING; HUMAN; HUMAN CELL; IMMUNOBLOTTING; MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; THAWING;

APOPTOSIS; BONE MARROW CELLS; CRYOPRESERVATION; ENDOPEPTIDASES; HUMAN; IMMUNOBLOTTING; IN VITRO; JURKAT CELLS; PROTEINS; SUPPORT, NON-U.S. GOV'T; TUMOR CELLS, CULTURED;

EID: 0034333232     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00285-4     Document Type: Article

References (7)

1. Cleavage of Bcl-2 is an early event in chemotherapy-induced apoptosis of human myeloid leukemia cells
2. Two pathways of apoptosis induced with all-trans retinoic acid and etoposide in the myeloid cell line P39
3. Caspase-mediated cleavage of cytoskeletal actin plays a positive role in the process of morphological apoptosis
4. Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of Jurkat cells
5. Apoptosis and its significance in MDS: Controversies revisited
6. Involvement of cellular proteolytic machinery in apoptosis