Generation of tetracycline-inducible mammalian cell lines by flow cytometry for improved overproduction of membrane proteins

Methods in Molecular Biology

Volumn 1432, Issue , 2016, Pages 63-78

  Andréll, Juni ^a   Edwards, Patricia C ^a   Zhang, Fan ^a   Daly, Maria ^a   Tate, Christopher G ^a  

^a NONE

Author keywords

Flow cytometry; GFP; GPCR; HEK293 cell line; Membrane protein; Overexpression; Transporter

Indexed keywords

GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN; TETRACYCLINE;

CELL ISOLATION; CELL POPULATION; CLONAL CELL LINE; FLOW CYTOMETRY; GENE EXPRESSION SYSTEM; HEK293 CELL LINE; MAMMAL CELL; METHODOLOGY; TISSUE CULTURE; ANIMAL; CELL CLONE; CELL CULTURE TECHNIQUE; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CYTOLOGY; GENETICS; HUMAN; METABOLISM; PROCEDURES; PROMOTER REGION; PROTEIN ENGINEERING;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CLONE CELLS; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; MEMBRANE PROTEINS; PROMOTER REGIONS, GENETIC; PROTEIN ENGINEERING; TETRACYCLINE;

EID: 84982732184     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3637-3_5     Document Type: Chapter

References (11)

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