Citrobacter freundii carrying bla KPC-2 and bla NDM-1: Characterization by whole genome sequencing

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Wu, Wenjing ^a   Espedido, Björn ^b   Feng, Yu ^a   Zong, Zhiyong ^a  

^a SICHUAN UNIVERSITY   (China)

^b UNIVERSITY OF WESTERN SYDNEY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIINFECTIVE AGENT; BETA LACTAMASE; BETA-LACTAMASE KPC-2; BETA-LACTAMASE NDM-1; CARBAPENEM DERIVATIVE;

ANALYSIS; BACTERIUM CONJUGATION; BETA-LACTAM RESISTANCE; CITROBACTER FREUNDII; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HORIZONTAL GENE TRANSFER; HOSPITAL; ISOLATION AND PURIFICATION; MICROBIOLOGY; PLASMID; SEWAGE; TRANSPOSON; WHOLE GENOME SEQUENCING;

ANTI-BACTERIAL AGENTS; BETA-LACTAM RESISTANCE; BETA-LACTAMASES; CARBAPENEMS; CITROBACTER FREUNDII; CONJUGATION, GENETIC; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; GENE TRANSFER, HORIZONTAL; HOSPITALS; PLASMIDS; SEWAGE; WHOLE GENOME SEQUENCING;

EID: 84979902248     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep30670     Document Type: Article

References (25)

1. Temkin, E., Adler, A., Lerner, A. & Carmeli, Y. Carbapenem-resistant Enterobacteriaceae: biology, epidemiology, and management. Ann N Y Acad Sci 1323, 22-42 (2014).
2. Pereira, P. S. et al. Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil. Microb Drug Resist 21, 234-236 (2015).
3. Quiles, M. G. et al. Unusual association of NDM-1 with KPC-2 and armA among Brazilian Enterobacteriaceae isolates. Braz J Med Biol Res 48, 174-177 (2015).
4. Kumarasamy, K. & Kalyanasundaram, A. Emergence of Klebsiella pneumoniae isolate co-producing NDM-1 with KPC-2 from India. J Antimicrob Chemother 67, 243-244 (2012).
5. Feng, J. et al. Coexistence of a novel KPC-2-encoding MDR plasmid and an NDM-1-encoding pNDM-HN380-like plasmid in a clinical isolate of Citrobacter freundii. J Antimicrob Chemother 70, 2987-2991 (2015).
6. Wu, W., Feng, Y., Carattoli, A. & Zong, Z. The emergence of Enterobacter cloacae producing both KPC and NDM carbapenemases: characterization by whole genome sequencing. Antimicrob Agents Chemother 59, 6625-6628 (2015).
7. Landman, D., Bratu, S. & Quale, J. Contribution of OmpK36 to carbapenem susceptibility in KPC-producing Klebsiella pneumoniae. J Med Microbiol 58, 1303-1308 (2009).
8. Tsai, Y. K. et al. Klebsiella pneumoniae outer membrane porins OmpK35 and OmpK36 play roles in both antimicrobial resistance and virulence. Antimicrob Agents Chemother 55, 1485-1493 (2011).
9. Petty, N. K. et al. The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli. J Bacteriol 192, 525-538 (2010).
10. Bauernfeind, A., Stemplinger, I., Jungwirth, R. & Giamarellou, H. Characterization of the plasmidic β-lactamase CMY-2, which is responsible for cephamycin resistance. Antimicrob Agents Chemother 40, 221-224 (1996).
11. Feng, Y. et al. Escherichia coli of sequence type 3835 carrying blaNDM-1, blaCTX-M-15, blaCMY-42 and blaSHV-12. Scientific Reports 5, 12275 (2015).
12. Ho, P. et al. Identification and characterization of a novel incompatibility group X3 plasmid carrying blaNDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China. Emerging Microbes and Infections 1, e39 (2012).
13. Espedido, B. A., Dimitrijovski, B., van Hal, S. J. & Jensen, S. O. The use of whole-genome sequencing for molecular epidemiology and antimicrobial surveillance: identifying the role of IncX3 plasmids and the spread of blaNDM-4-like genes in the Enterobacteriaceae. J Clin Pathol 68, 835-838 (2015).
14. Zong, Z. & Zhang, X. blaNDM-1-carrying Acinetobacter johnsonii detected in hospital sewage. J Antimicrob Chemother 68, 1007-1010 (2013).
15. Mendes, R. E. et al. Rapid detection and identification of metallo-β-lactamase-encoding genes by multiplex real-Time PCR assay and melt curve analysis. J Clin Microbiol 45, 544-547 (2007).
16. Poirel, L., Le Thomas, I., Naas, T., Karim, A. & Nordmann, P. Biochemical sequence analyses of GES-1, a novel class A extendedspectrum β-lactamase, and the class 1 integron In52 from Klebsiella pneumoniae. Antimicrob Agents Chemother 44, 622-632 (2000).
17. Bradford, P. A. et al. Emergence of carbapenem-resistant Klebsiella species possessing the class A carbapenem-hydrolyzing KPC-2 and inhibitor-resistant TEM-30 β-lactamases in New York City. Clin Infect Dis 39, 55-60 (2004).
18. Yamamoto, S. & Harayama, S. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 61, 1104-1109 (1995).
19. CLSI. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. M100-S23. Clinical and Laboratory Standards Institute (2013).
20. EUCAST. Clinical breakpoints-bacteria, v5.0. The European Committee on Antimicrobial Susceptibility Testing (2015).
21. Bankevich, A. et al. SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19, 455-477 (2012).
22. Seemann, T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30, 2068-2069 (2014).
23. Treangen, T. J., Ondov, B. D., Koren, S. & Phillippy, A. M. The Harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes. Genome Biol 15, 524 (2014).
24. Richter, M., Rossello-Mora, R., Oliver Glockner, F. & Peplies, J. JSpeciesWS: A web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics (2015).
25. Carattoli, A. et al. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods 63, 219-228 (2005).