Real-time quantitative PCR assay with Taqman® probe for rapid detection of MCR-1 plasmid-mediated colistin resistance

New Microbes and New Infections

Volumn 13, Issue , 2016, Pages 71-74

  Chabou, S ^a   Leangapichart, T ^a   Okdah, L ^a   Le Page, S ^a   Hadjadj, L ^a   Rolain, J M ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Antibiotic resistance surveillance; colistin resistance; mcr 1 gene; PCR detection, Taqman probe

Indexed keywords

MCR 1 GENE ENCODING PROTEIN; MEMBRANE PROTEIN; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; BROILER; COLONY FORMING UNIT; CONTROLLED STUDY; ENTEROBACTER CLOACAE; ESCHERICHIA COLI; FECES ANALYSIS; GENE; GENE DOSAGE; GENE SEQUENCE; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; MCR 1 GENE; MOLECULAR BEACON; MORGANELLA MORGANII; NONHUMAN; NOTE; PRIORITY JOURNAL; PROTEUS MIRABILIS; PROTEUS VULGARIS; PROVIDENCIA RETTGERI; PSEUDOMONAS AERUGINOSA; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA ENTERICA; SENSITIVITY AND SPECIFICITY; SERRATIA MARCESCENS; TAQMAN PROBE;

EID: 84979642777     PISSN: 20522975     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.nmni.2016.06.017     Document Type: Note

References (18)

1. [1] Biswas, S., Brunel, J.M., Dubus, J.C., Reynaud-Gaubert, M., Rolain, J.M., Colistin: an update on the antibiotic of the 21st century. Expert Rev Anti Infect Ther 10 (2012), 917–934.
2. [2] Rolain, J.M., Olaitan, A.O., Plasmid-mediated colistin resistance: the final blow to colistin?. Int J Antimicrob Agents 47 (2016), 4–5.
3. [3] Olaitan, A.O., Morand, S., Rolain, J.M., Mechanisms of polymyxin resistance: acquired and intrinsic resistance in bacteria. Front Microbiol, 5, 2014, 643.
4. [4] Liu, Y.Y., Wang, Y., Walsh, T.R., Yi, L.X., Zhang, R., Spencer, J., et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 16 (2016), 161–168.
5. [5] Olaitan, A.O., Chabou, S., Okdah, L., Morand, S., Rolain, J.M., Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis, 16, 2016, 147.
6. [6] Kempf, I., Fleury, M.A., Drider, D., Bruneau, M., Sanders, P., Chauvin, C., et al. What do we know about resistance to colistin in Enterobacteriaceae in avian and pig production in Europe?. Int J Antimicrob Agents 42 (2013), 379–383.
7. [7] Olaitan, A.O., Thongmalayvong, B., Akkhavong, K., Somphavong, S., Paboriboune, P., Khounsy, S., et al. Clonal transmission of a colistin-resistant Escherichia coli from a domesticated pig to a human in Laos. J Antimicrob Chemother 70 (2015), 3402–3404.
8. [8] Olaitan, A.O., Morand, S., Rolain, J.M., Emergence of colistin-resistant bacteria in humans without colistin usage: a new worry and cause for vigilance. Int J Antimicrob Agents 47 (2016), 1–3.
9. [9] Olaitan, A.O., Diene, S.M., Kempf, M., Berrazeg, M., Bakour, S., Gupta, S.K., et al. Worldwide emergence of colistin resistance in Klebsiella pneumoniae from healthy humans and patients in Lao PDR, Thailand, Israel, Nigeria and France owing to inactivation of the PhoP/PhoQ regulator mgrB: an epidemiological and molecular study. Int J Antimicrob Agents 44 (2014), 500–507.
10. [10] Olaitan, A.O., Dia, N.M., Gautret, P., Benkouiten, S., Belhouchat, K., Drali, T., et al. Acquisition of extended-spectrum cephalosporin- and colistin-resistant Salmonella enterica subsp. enterica serotype Newport by pilgrims during Hajj. Int J Antimicrob Agents 45 (2015), 600–604.
11. [11] Al Bayssari, C., Dabboussi, F., Hamze, M., Rolain, J.M., Emergence of carbapenemase-producing Pseudomonas aeruginosa and Acinetobacter baumannii in livestock animals in Lebanon. J Antimicrob Chemother 70 (2015), 950–951.
12. [12] Bakour, S., Kempf, M., Touati, A., Ait, A.A., Haouchine, D., Sahli, F., et al. Carbapenemase-producing Acinetobacter baumannii in two university hospitals in Algeria. J Med Microbiol 61 (2012), 1341–1343.
13. [13] Bakour, S., Touati, A., Bachiri, T., Sahli, F., Tiouit, D., Naim, M., et al. First report of 16S rRNA methylase ArmA-producing Acinetobacter baumannii and rapid spread of metallo-β-lactamase NDM-1 in Algerian hospitals. J Infect Chemother 20 (2014), 696–701.
14. [14] Rolain, J.M., Diene, S.M., Kempf, M., Gimenez, G., Robert, C., Raoult, D., Real-time sequencing to decipher the molecular mechanism of resistance of a clinical pan-drug-resistant Acinetobacter baumannii isolate from Marseille, France. Antimicrob Agents Chemother 57 (2013), 592–596.
15. [15] Olaitan, A.O., Diene, S.M., Assous, M.V., Rolain, J.M., Genomic plasticity of multidrug-resistant NDM-1 positive clinical isolate of Providencia rettgeri. Genome Biol Evol 8 (2015), 723–728.
16. [16] Olaitan, A.O., Diene, S.M., Gupta, S.K., Adler, A., Assous, M.V., Rolain, J.M., Genome analysis of NDM-1 producing Morganella morganii clinical isolate. Expert Rev Anti Infect Ther 12 (2014), 1297–1305.
17. [17] Rutledge, R.G., Cote, C., Mathematics of quantitative kinetic PCR and the application of standard curves. Nucleic Acids Res, 31, 2003, e93.
18. [18] Bontron, S., Poirel, L., Nordmann, P., Real-time PCR for detection of plasmid-mediated polymyxin resistance (mcr-1) from cultured bacteria and stools. J Antimicrob Chemother, 2016 Apr 27.