16S rRNA gene sequencing of mock microbial populations-impact of DNA extraction method, primer choice and sequencing platform

BMC Microbiology

Volumn 16, Issue 1, 2016, Pages

  Fouhy, Fiona ^a   Clooney, Adam G ^b,c   Stanton, Catherine ^a,c   Claesson, Marcus J ^b,c   Cotter, Paul D ^a,c  

^a TEAGASC FOOD RESEARCH CENTRE   (Ireland)

^b UNIVERSITY COLLEGE CORK   (Ireland)

^c UNIVERSITY COLLEGE CORK   (Ireland)

Author keywords

16S rRNA; Bias; DNA extraction; Gut microbiota; Ion PGM; MiSeq; Mock communities; Next generation sequencing

Indexed keywords

DNA; RNA 16S; BACTERIAL DNA; PRIMER DNA;

16S RRNA GENE; ACCURACY; ARTICLE; BACTERIAL GENE; CLOSTRIDIUM; CONTROLLED STUDY; DNA EXTRACTION; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; GENE AMPLIFICATION; GENE SEQUENCE; LISTERIA; MICROBIAL COMMUNITY; MICROFLORA; NEXT GENERATION SEQUENCING; NONHUMAN; SPECIES IDENTIFICATION; STREPTOCOCCUS; BACTERIUM; CLASSIFICATION; GENETICS; HIGH THROUGHPUT SEQUENCING; ISOLATION AND PURIFICATION;

BACTERIA; DNA PRIMERS; DNA, BACTERIAL; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MICROBIOTA; RNA, RIBOSOMAL, 16S;

EID: 84978286049     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/s12866-016-0738-z     Document Type: Article

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