Crystal Violet and XTT Assays on Staphylococcus aureus Biofilm Quantification

Current Microbiology

Volumn 73, Issue 4, 2016, Pages 474-482

  Xu, Zhenbo ^a,b   Liang, Yanrui ^a   Lin, Shiqi ^a   Chen, Dingqiang ^c   Li, Bing ^a   Li, Lin ^a   Deng, Yang ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^b UNIVERSITY OF MARYLAND   (United States)

^c GUANGZHOU MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL RNA; CRYSTAL VIOLET; RIBOSOME RNA; RNA 16S; TETRAZOLIUM;

ARTICLE; ASSAY; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BIOFILM; COAGULASE NEGATIVE STAPHYLOCOCCUS; CONTROLLED STUDY; CRYSTAL VIOLET ASSAY; LATEX AGGLUTINATION TEST; METHICILLIN RESISTANT COAGULASE NEGATIVE STAPHYLOCOCCUS; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN SUSCEPTIBLE COAGULASE NEGATIVE STAPHYLOCOCCUS; METHICILLIN SUSCEPTIBLE STAPHYLOCOCCUS AUREUS; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; XTT ASSAY; CHEMISTRY; EVALUATION STUDY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; PHYSIOLOGY; PROCEDURES; STAINING; STAPHYLOCOCCUS INFECTION;

BIOFILMS; GENTIAN VIOLET; HUMANS; STAINING AND LABELING; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; TETRAZOLIUM SALTS;

EID: 84975257295     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-016-1081-1     Document Type: Article

References (24)

1. Beenken KE, Dunman PM, McAleese F, Macapagal D, Murphy E, Projan SJ, Blevins JS, Smeltzer MS (2004) Global gene expression in Staphylococcus aureus biofilms. J Bacteriol 186(14):4665–4684
2. Cattelan N, Dubey P, Arnal L, Yantorno OM, Deora R (2016) Bordetella biofilms: a lifestyle leading to persistent infections. Pathogens Dis 74(1):ftv108
3. Christensen GD, Simpson W, Younger J, Baddour L, Barrett F, Melton D, Beachey E (1985) Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol 22(6):996–1006
4. Claessens J, Roriz M, Merckx R, Baatsen P, Van Mellaert L, Van Eldere J (2015) Inefficacy of vancomycin and teicoplanin in eradicating and killing Staphylococcus epidermidis biofilms in vitro. Int J Antimicrob Agents 45(4):368–375
5. Corrigan RM, Rigby D, Handley P, Foster TJ (2007) The role of Staphylococcus aureus surface protein SasG in adherence and biofilm formation. Microbiology 153(8):2435–2446
6. Costerton JW (1999) Introduction to biofilm. Int J Antimicrob Agents 11(3):217–221
7. Cramton SE, Gerke C, Schnell NF, Nichols WW, Götz F (1999) The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation. Infect Immun 67(10):5427–5433
8. Cucarella C, Solano C, Valle J, Amorena B, Lasa Í, Penadés JR (2001) Bap, a Staphylococcus aureus surface protein involved in biofilm formation. J Bacteriol 183(9):2888–2896
9. da Silva Meira QG, de Medeiros Barbosa I, Athayde AJAA, de Siqueira-Júnior JP, de Souza EL (2012) Influence of temperature and surface kind on biofilm formation by Staphylococcus aureus from food-contact surfaces and sensitivity to sanitizers. Food Control 25(2):469–475
10. Donlan RM, Costerton JW (2002) Biofilms: survival mechanisms of clinically relevant microorganisms. Clin Microbiol Rev 15(2):167–193
11. Flemming H-C, Wingender J (2010) The biofilm matrix. Nat Rev Microbiol 8(9):623–633
12. Gill SR, Fouts DE, Archer GL, Mongodin EF, DeBoy RT, Ravel J, Paulsen IT, Kolonay JF, Brinkac L, Beanan M (2005) Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain. J Bacteriol 187(7):2426–2438
13. Hall-Stoodley L, Costerton JW, Stoodley P (2004) Bacterial biofilms: from the natural environment to infectious diseases. Nat Rev Microbiol 2(2):95–108
14. Kong K-F, Vuong C, Otto M (2006) Staphylococcus quorum sensing in biofilm formation and infection. Int J Med Microbiol 296(2):133–139
15. Kumar CG, Anand S (1998) Significance of microbial biofilms in food industry: a review. Int J Food Microbiol 42(1):9–27
16. Lim Y, Shin HJ, Kwon AS, Reu JH, Park G, Kim J (2013) Predictive genetic risk markers for strong biofilm-forming Staphylococcus aureus: fnbB gene and SCCmec type III. Diagn Microbiol Infect Dis 76(4):539–541
17. Mizan MFR, Jahid IK, Ha S-D (2015) Microbial biofilms in seafood: a food-hygiene challenge. Food Microbiol 49:41–55
18. O’Neill E, Pozzi C, Houston P, Smyth D, Humphreys H, Robinson DA, O’Gara JP (2007) Association between methicillin susceptibility and biofilm regulation in Staphylococcus aureus isolates from device-related infections. J Clin Microbiol 45(5):1379–1388
19. Peeters E, Nelis HJ, Coenye T (2008) Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Methods 72(2):157–165
20. Pitts B, Hamilton MA, Zelver N, Stewart PS (2003) A microtiter-plate screening method for biofilm disinfection and removal. J Microbiol Methods 54(2):269–276
21. Potera C (1999) Forging a link between biofilms and disease. Science 283(5409):1837–1839
22. Scudiero DA, Shoemaker RH, Paull KD, Monks A, Tierney S, Nofziger TH, Currens MJ, Seniff D, Boyd MR (1988) Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res 48(17):4827–4833
23. Silva WJd, Seneviratne J, Parahitiyawa N, Rosa EAR, Samaranayake LP, Cury AADB (2008) Improvement of XTT assay performance for studies involving Candida albicans biofilms. Braz Den J 19(4):364–369
24. Simões M, Simões LC, Vieira MJ (2010) A review of current and emergent biofilm control strategies. LWT-Food Sci Technol 43(4):573–583