TT-seq maps the human transient transcriptome

Science

Volumn 352, Issue 6290, 2016, Pages 1225-1228

  Schwalb, Björn ^a   Michel, Margaux ^a   Zacher, Benedikt ^b   Hauf, KatjaFrü ^c   Demel, Carina ^a   Tresch, Achim ^d,e   Gagneur, Julien ^a,b,f   Cramer, Patrick ^a,c  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

^c KAROLINSKA INSTITUTE   (Sweden)

^d UNIVERSITY OF COLOGNE   (Germany)

^e MAX PLANCK INSTITUTE FOR PLANT BREEDING RESEARCH   (Germany)

^f TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY RNA; INITIATION FACTOR; LONG UNTRANSLATED RNA; MESSENGER RNA; RNA POLYMERASE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR IIB; TRANSCRIPTION FACTOR IIF; TRANSCRIPTOME; UNCLASSIFIED DRUG; DNA DIRECTED RNA POLYMERASE;

DEGRADATION; DNA; ESTIMATION METHOD; GENOME; RNA;

ARTICLE; CHROMATIN IMMUNOPRECIPITATION; ENHANCER REGION; GENE MAPPING; GENETIC BACKGROUND; HALF LIFE TIME; HISTONE MODIFICATION; HUMAN; HUMAN CELL; HUMAN GENOME; K562 CELL LINE; POLYADENYLATION; PRIORITY JOURNAL; PROMOTER REGION; RNA ANALYSIS; RNA DEGRADATION; RNA SEQUENCE; RNA STRUCTURE; RNA SYNTHESIS; RNA TRANSCRIPTION; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION TERMINATION; TRANSIENT TRANSCRIPTOME SEQUENCING; BASE PAIRING; GENE EXPRESSION PROFILING; GENETICS; METABOLISM; REGULATOR GENE;

BASE PAIRING; DNA-DIRECTED RNA POLYMERASES; GENE EXPRESSION PROFILING; HUMANS; POLYADENYLATION; PROMOTER REGIONS, GENETIC; RNA, LONG NONCODING; RNA, MESSENGER; TERMINATOR REGIONS, GENETIC; TRANSCRIPTION TERMINATION, GENETIC; TRANSCRIPTOME;

EID: 84975072539     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aad9841     Document Type: Article

References (25)

1. T.H.Jensen,A.Jacquier,D.Libri,Mol.Cell52,473-484(2013).
2. R.Anderssonetal.,Nature507,455-461(2014).
3. M.D.Cleary,C.D.Meiering,E.Jan,R.Guymon,J.C.Boothroyd, Nat.Biotechnol.23,232-237(2005).
4. Materials and methods are available as supplementary materialson Science Online
5. L.J.Coreetal.,Nat.Genet.46,1311-1320(2014).
6. R.Kodziusetal.,Nat.Methods3,211-222(2006).
7. H.Kwak,N.J.Fuda,L.J.Core,J.T.Lis,Science339,950-953 (2013).
8. A.Mayeretal.,Cell161,541-554(2015).
9. T.Nojimaetal.,Cell161,526-540(2015).
10. L.J.Core,J.J.Waterfall,J.T.Lis,Science322,1845-1848 (2008).
11. B.Zacheretal.,http://biorxiv.org/content/early/2016/03/06/ 041020(2016).
12. J.Harrowetal.,GenomeRes.22,1760-1774(2012).
13. S.Djebalietal.,Nature489,101-108(2012).
14. T.K.Kimetal.,Nature465,182-187(2010).
15. ENCODEProjectConsortium,Nature489,57-74(2012).
16. R.A.Flynn,A.E.Almada,J.R.Zamudio,P.A.Sharp,Proc. Natl.Acad.Sci.U.S.A.108,10460-10465(2011).
17. M.Rabanietal.,Cell159,1698-1710(2014).
18. D.Kaidaetal.,Nature468,664-668(2010).
19. M.G.Bergetal.,Cell150,53-64(2012).
20. A.E.Almada,X.Wu,A.J.Kriz,C.B.Burge,P.A.Sharp,Nature 499,360-363(2013).
21. S.West,N.Gromak,N.J.Proudfoot,Nature432,522-525 (2004).
22. M.Kimetal.,Nature432,517-522(2004).
23. B.J.Venters,B.F.Pugh,Nature502,53-58(2013).
24. R.Ashfieldetal.,EMBOJ.13,5656-5667(1994).
25. M.L.Kireeva,N.Komissarova,D.S.Waugh,M.Kashlev,J.Biol. Chem.275,6530-6536(2000).