Visualization of Stat3 and Stat5 transactivation activity with specific response element dependent reporter constructs integrated into lentiviral gene transfer vectors

Hormone Molecular Biology and Clinical Investigation

Volumn 1, Issue 3, 2010, Pages 127-137

  Gäbel, Katrin ^a   Bednorz, Nadja Lydia ^a   Klemmt, Petra ^a   Vafaizadeh, Vida ^a   Borghouts, Corina ^a   Groner, Bernd ^a,b  

^a Institute for Biomedical Research   (Germany)

^b INSTITUTE FOR TUMOR BIOLOGY AND EXPERIMENTAL THERAPY   (Germany)

Author keywords

lentiviral gene transfer vectors; reporter genes; Stat3; Stat5

Indexed keywords

BETA GALACTOSIDASE; FLUORESCENT DYE; INTERLEUKIN 6; INTERLEUKIN 6 RECEPTOR; LENTIVIRUS VECTOR; MCHERRY PROTEIN; PROLACTIN RECEPTOR; STAT3 PROTEIN; STAT5 PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BREAST EPITHELIUM CELL; CANCER CELL; CELL CULTURE; CELLS BY BODY ANATOMY; CONTROLLED STUDY; DNA RESPONSIVE ELEMENT; ENZYME ACTIVITY; FAT PAD; FEMALE; FLUORESCENCE ACTIVATED CELL SORTING; GENE CONSTRUCT; GENE TRANSFER; GLIOBLASTOMA CELL LINE; HEP-G2 CELL LINE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; REPORTER GENE; THIRD TRIMESTER PREGNANCY; TRANSACTIVATION; TRANSIENT TRANSFECTION; VIRUS PARTICLE;

EID: 84973931994     PISSN: 18681883     EISSN: 18681891     Source Type: Journal    
DOI: 10.1515/HMBCI.2010.017     Document Type: Article

References (39)

1. Hennighausen L., Robinson GW. Interpretation of cytokine signaling through the transcription factors STAT5A and STAT5B. Genes Dev 2008;22:711-21.
2. Liu S., Dontu G., Wicha MS. Mammary stem cells., self-renewal pathways., and carcinogenesis. Breast Cancer Res 2005;7:86-95.
3. Pawson T., Kofler M. Kinome signaling through regulated protein-protein interactions in normal and cancer cells. Curr Opin Cell Biol 2009;21:147-53.
4. Brierley MM., Fish EN. Stats: multifaceted regulators of transcription. J Interferon Cytokine Res 2005;25:733-44.
5. Desrivieres S., Kunz C., Barash I., Vafaizadeh V., Borghouts C., Groner B. The biological functions of the versatile transcription factors STAT3 and STAT5 and new strategies for their targeted inhibition. J Mammary Gland Biol Neoplasia 2006;11:75-87.
6. Akira S., Nishio Y., Inoue M., Wang XJ., Wei S., Matsusaka T., Yoshida K., Sudo T., Naruto M., Kishimoto T. Molecular cloning of APRF., a novel IFN-stimulated gene factor 3 p91-related transcription factor involved in the gp130-mediated signaling pathway. Cell 1994;77:63-71.
7. Levy DE., Darnell JE Jr. Stats: transcriptional control and biological impact. Nat Rev 2002;3:651-62.
8. Stepkowski SM., Chen W., Ross JA., Nagy ZS., Kirken RA. STAT3: an important regulator of multiple cytokine functions. Transplantation 2008;85:1372-7.
9. Brantley EC., Benveniste EN. Signal transducer and activator of transcription-3: a molecular hub for signaling pathways in glio-mas. Mol Cancer Res 2008;6:675-84.
10. Wakao H., Gouilleux F., Groner B. Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response. EMBO J 1994; 13:2182-91.
11. Wakao H., Schmitt-Ney M., Groner B. Mammary gland-specific nuclear factor is present in lactating rodent and bovine mammary tissue and composed of a single polypeptide of 89 kDa. J Biol Chem 1992;267:16365-70.
12. Gouilleux F., Wakao H., Mundt M., Groner B. Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription. EMBO J 1994;13: 4361-9.
13. Wakao H., Gouilleux F., Groner B. Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response. EMBO J 1995; 14:854-5.
14. Park SH., Liu X., Hennighausen L., Davey HW., Waxman DJ. Distinctive roles of STAT5a and STAT5b in sexual dimorphism of hepatic P450 gene expression. Impact of STAT5a gene disruption. J Biol Chem 1999;274:7421-30.
15. Wierenga AT., Vellenga E., Schuringa JJ. Maximal STAT5-induced proliferation and self-renewal at intermediate STAT5 activity levels. Mol Cell Biol 2008;28:6668-80.
16. Hoelbl A., Kovacic B., Kerenyi MA., Simma O., Warsch W., Cui Y., Beug H., Hennighausen L., Moriggl R., Sexl V. Clarifying the role of Stat5 in lymphoid development and Abelson-induced transformation. Blood 2006;107:4898-906.
17. Ihle JN. The Stat family in cytokine signaling. Curr Opin Cell Biol 2001;13:211-7.
18. Kang JH., Chung JK. Molecular-genetic imaging based on reporter gene expression. J Nucl Med 2008;49(Suppl 2):164S-79S.
19. Serganova I., Mayer-Kukuck P., Huang R., Blasberg R. Molecular imaging: reporter gene imaging. Handb Exp Pharmacol 2008; 185(Pt 2):167-223.
20. Weber K., Bartsch U., Stocking C., Fehse B. A multicolor panel of novel lentiviral “gene ontology” (LeGO) vectors for functional gene analysis. Mol Ther 2008;16:698-706.
21. Schmidt-Ullrich R., Memet S., Lilienbaum A., Feuillard J., Raphael M., Israel A. NF-kappaB activity in transgenic mice: developmental regulation and tissue specificity. Development 1996;122:2117-28.
22. DasGupta R., Fuchs E. Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and differentiation. Development 1999;126:4557-68.
23. Maretto S., Cordenonsi M., Dupont S., Braghetta P., Broccoli V., Hassan AB., Volpin D., Bressan GM., Piccolo S. Mapping Wnt/ beta-catenin signaling during mouse development and in colo-rectal tumors. Proc Natl Acad Sci USA 2003;100:3299-304.
24. Davies PS., Dismuke AD., Powell AE., Carroll KH., Wong MH. Wnt-reporter expression pattern in the mouse intestine during homeostasis. BMC Gastroenterol 2008;8:57.
25. Zangani M., Carlsen H., Kielland A., Os A., Hauglin H., Blomhoff R., Munthe LA., Bogen B. Tracking early autoimmune disease by bioluminescent imaging of NF-kappaB activation reveals pathology in multiple organ systems. Am J Pathol 2009;174: 1358-67.
26. Singer O., Verma IM. Applications of lentiviral vectors for shRNA delivery and transgenesis. Curr Gene Ther 2008;8: 483-8.
27. Frecha C., Szecsi J., Cosset FL., Verhoeyen E. Strategies for targeting lentiviral vectors. Curr Gene Ther 2008;8:449-60.
28. Stocklin E., Wissler M., Gouilleux F., Groner B. Functional interactions between Stat5 and the glucocorticoid receptor. Nature 1996;383:726-8.
29. Naldini L., Blomer U., Gage FH., Trono D., Verma IM. Efficient transfer., integration., and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci USA 1996;93:11382-8.
30. Zufferey R., Nagy D., Mandel RJ., Naldini L., Trono D. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat Biotechnol 1997;15:871-5.
31. Clevenger CV. Roles and regulation of stat family transcription factors in human breast cancer. Am J Pathol 2004;165:1449-60.
32. Groner B., Lucks P., Borghouts C. The function of Stat3 in tumor cells and their microenvironment. Semin Cell Dev Biol 2008;19: 341-50.
33. Haura EB., Turkson J., Jove R. Mechanisms of disease: insights into the emerging role of signal transducers and activators of transcription in cancer. Nat Clin Pract Oncol 2005;2:315-24.
34. Maurer-Gebhard M., Schmidt M., Azemar M., Altenschmidt U., Stocklin E., Wels W., Groner B. Systemic treatment with a recom binant erbB-2 receptor-specific tumor toxin efficiently reduces pulmonary metastases in mice injected with genetically modified carcinoma cells. Cancer Res 1998;58:2661-6.
35. Berchtold S., Moriggl R., Gouilleux F., Silvennoinen O., Beisen-herz C., Pfitzner E., Wissler M., Stocklin E., Groner B. Cytokine receptor-independent., constitutively active variants of STAT5. J Biol Chem 1997;272:30237-43.
36. Cockrell AS., Kafri T. Gene delivery by lentivirus vectors. Mol Biotechnol 2007;36:184-204.
37. Yue P., Turkson J. Targeting STAT3 in cancer: how successful are we? Expert Opin Investig Drugs 2009;18:45-56.
38. Lois C., Hong EJ., Pease S., Brown EJ., Baltimore D. Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 2002;295:868-72.
39. Pfeifer A., Hofmann A. Lentiviral transgenesis. Methods Mol Biol 2009;530:391-405.