High content screening analysis to evaluate the toxicological effects of Harmful and Potentially Harmful Constituents (HPHC)

Journal of Visualized Experiments

Volumn 2016, Issue 111, 2016, Pages

  Marescotti, Diego ^a   Suarez, Ignacio Gonzalez ^a   Acali, Stefano ^a   Johne, Stephanie ^a   Laurent, Alexandra ^a   Frentzel, Stefan ^a   Hoeng, Julia ^a   Peitsch, Manuel C ^a  

^a PHILIP MORRIS PRODUCTS S A   (Switzerland)

Author keywords

Cell stress; DNA damage; High content screening; Issue 111; Molecular Biology; Normal Human Bronchial Epithelial cells; Smoke Constituents; Systems Toxicology

Indexed keywords

AEROSOL; SMOKE;

AEROSOL; PROCEDURES; RISK FACTOR; SMOKE; TOBACCO; TOXICOLOGY;

AEROSOLS; RISK FACTORS; SMOKE; TOBACCO; TOXICOLOGY;

EID: 84971516660     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/53987     Document Type: Article

References (14)

1. Rodgman, A., & Perfetti, T.A. The chemical components of tobacco and tobacco smoke. CRC Press, Boca Raton, FL, (2013).
2. Russell, W.M.S. and Burch, R.L. The Principles of Humane Experimental Technique. Methuen, London, (1959).
3. Zock, J.M. Applications of high content screening in life science research combinatorial chemistry & high throughput screening. Comb. Chem. High. Throughput. Screen. 132 (9), 870-876 (2009).
4. US Food and Drug Administration. Harmful and potentially harmful constituents in tobacco products and tobacco smoke; established list, federal register. 20034-20037, (2012).
5. Gonzalez-Suarez, I. Systems Biology Approach for Evaluating the Biological Impact of Environmental Toxicants in Vitro. Chem. Res. Toxicol. 27 (3) 367-376, (2014).
6. Camilleri-Broet, S., Vanderwerff H., Caldwell E., & Hockenbery D. Distinct alterations in mitochondrial mass and function characterize different models of apoptosis. Exp. Cell. Res. 239 (2), 277-292 (1998).
7. Li, P. et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates and apoptotic protease cascade. Cell. 91 (4), 479-489 (1997).
8. Rogakou, E.P., Pilch, D.R., Orr, A.H., Ivanova, V.S., & Bonner, W.M. Double strand breaks induce histone H2AX phosphorylation on serine 139. J. Biol. Chem. 273 (10) 5858-5868, (1998).
9. Westwick, J.K., Weitzel C., Minden A., Karin M., & Brenner D.A. Tumor necrosis factor α stimulates AP-1 activity through prolonged activation of the c-jun kinase. J. Biol. Chem. 269 (42) 26396-26401, (1994).
10. Bindokas, V.P., Jordàn J., Lee C.C., & Miller R.J. Superoxide production in rat hippocampal neurons: selective imaging with hydroethidine. J. Neurosci. 16 (4) 1324-1336, (1996).
11. Barhoumi, R., Bailey R.H., & Burghardt R.C. Kinetic analysis of glutathione in anchored cells with monochlorobimane. J. Cytometry. 19 (3) 226-234, (1994).
12. Huang, T.C., Lee J.F., & Chen J.Y. Pardaxin, an antimicrobial peptide, triggers caspase-dependent and ROS-mediated apoptosis in HT-1080 Cells. Mar. Drugs. 9 (10) 1995-2009, (2011).
13. Bao, S., & Knoell D.L. Zinc modulates cytokine-induced lung epithelial cell barrier permeability. Am. J. Physiol. Lung Cell Mol. Physiol. 291 (6) L1132-1141, (2006).
14. Xia, M. et al. Compound Cytotoxicity Profiling Using Quantitative High-Throughput Screening. Environ Health Perspect. 116 (3) 284-291, (2008).