High production of 3-hydroxypropionic acid in klebsiella pneumoniae by systematic optimization of glycerol metabolism

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Li, Ying ^a,b   Wang, Xi ^a   Ge, Xizhen ^b   Tian, Pingfang ^a  

^a BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY   (China)

^b BEIJING UNION UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ACETIC ACID; ALDEHYDE DEHYDROGENASE; BACTERIAL PROTEIN; GLYCEROL; HYDRACRYLIC ACID; ISOPROPYL THIOGALACTOSIDE; LACTIC ACID;

ANALOGS AND DERIVATIVES; BIOSYNTHESIS; DRUG EFFECT; FERMENTATION; GENE DELETION; GENE EXPRESSION REGULATION; GENETICS; KINETICS; KLEBSIELLA PNEUMONIAE; METABOLIC ENGINEERING; METABOLISM; PROMOTER REGION;

ACETIC ACID; ALDEHYDE DEHYDROGENASE; BACTERIAL PROTEINS; FERMENTATION; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; GLYCEROL; ISOPROPYL THIOGALACTOSIDE; KINETICS; KLEBSIELLA PNEUMONIAE; LACTIC ACID; METABOLIC ENGINEERING; PROMOTER REGIONS, GENETIC;

EID: 84971350787     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep26932     Document Type: Article

References (31)

1. Werpy, T., & Petersen, G. Top value added chemicals from biomass. U.S. DOE, Washington, DC (2004).
2. Andreessen, B., Lange, A. B., Robenek, H., & Steinbuchel, A. Conversion of glycerol to poly(3-hydroxypropionate) in recombinant Escherichia coli. Appl. Environ. Microbiol. 76(2), 622-626 (2010).
3. Zhou, Q., et al. Production of 3-hydroxypropionate homopolymer and poly(3-hydroxypropionate-co-4-hydroxybutyrate) copolymer by recombinant Escherichia coli. Metab. Eng. 13(6), 777-785 (2011).
4. Kumar, V., Ashok, S., & Park, S. Recent advances in biological production of 3-hydroxypropionic acid. Biotechnol. Adv. 31, 945-961 (2013).
5. Menzel, K., Zeng, A., & Deckwer, W. D. High concentration and productivity of 1, 3-propanediol from continuous fermentation of glycerol by Klebsiella pneumoniae. Enzyme. Microb. Technol. 20, 82-86 (2008).
6. Raj, S. M., Rathnasingh, C., Jo, J. E., & Park, S. Production of 3-hydroxypropionic acid from glycerol by a novel recombinant Escherichia coli BL21 strain. Process. Biochem. 43, 1440-1446 (2008).
7. Chu, H. S., et al. Metabolic engineering of 3-hydroxypropionic acid biosynthesis in Escherichia coli. Biotechnol. Bioeng. 112(2), 356-364 (2015).
8. Forage, R. G., & Lin, E. C. DHA system mediating aerobic and anaerobic dissimilation of glycerol in Klebsiella pneumoniae NCIB 418. J. Bacteriol. 151(2), 591-599 (1982).
9. Johnson, E. A., & Lin, E. C. Klebsiella pneumoniae 1, 3-propanediol: NAD+ oxidoreductase. J. Bacteriol. 169(5), 2050-2054 (1987).
10. Celinska, E. Debottlenecking the 1, 3-propanediol pathway by metabolic engineering. Biotechnol. Adv. 28(4), 519-530 (2010).
11. Wang, Y., Tao, F., & Xu, P. Glycerol dehydrogenase plays a dual role in glycerol metabolism and 2, 3-butanediol formation in Klebsiella pneumoniae. J. Biol. Chem. 289, 6080-6090 (2014).
12. Rathnasingh, C., Raj, S. M., Jo, J. E., & Park, S. Development and evaluation of efficient recombinant Escherichia coli strains for the production of 3-hydroxypropionic acid from glycerol. Biotechnol. Bioeng. 104(4), 729-739 (2009).
13. Ko, Y., Ashok, S., Zhou, S., Kumar, V., & Park, S. Aldehyde dehydrogenase activity is important to the production of 3-hydroxypro pionic acid from glycerol by recombinant Klebsiella pneumoniae. Process. Biochem. 47, 1135-1143 (2012).
14. Li, Y., Su, M., Ge, X., & Tian, P. Enhanced aldehyde dehydrogenase activity by regenerating NAD+ In Klebsiella pneumoniae and implications for the glycerol dissimilation pathways. Biotechnol. Lett. 35(10), 1609-1615 (2013).
15. Raj, S. M., Rathnasingh, C., Jung, W. C., Selvakumar, E., & Park, S. A novel NAD+-dependent aldehyde dehydrogenase encoded by the puuC gene of Klebsiella pneumoniae DSM 2026 that utilizes 3-hydroxypropionaldehyde as a substrate. Biotechnol. Bioprocess. Eng. 15(1), 131-138 (2010).
16. De Boer, H. A., Comstock, L. J., & Vasser, M. The tac promoter: a functional hybrid derived from the trp and lac promoters. Proc. Natl. Acad. Sci. USA 80(1), 21-25 (1983).
17. Bagdasarian, M. M., Amann, E., Lurz, R., Ruckert, B., & Bagdasarian, M. Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors. Gene 26(2-3), 273-282 (1983).
18. Nakamura, K., Ishida, H., & Iizumi, T. Constitutive trichloroethylene degradation led by tac promoter chromosomally integrated upstream of phenol hydroxylase genes of Ralstonia sp. KN1 and its nucleotide sequence analysis. J. Biosci. Bioeng. 89(1), 47-54 (2000).
19. Wang, X., Sa, N., Wang, F., & Tian, P. Engineered constitutive pathway in Klebsiella pneumoniae for 3-hydroxypropionic acid production and implications for decoupling glycerol dissimilation pathways. Curr. Microbiol. 66(3), 293-299 (2013).
20. Kumar, V., et al. Simultaneous production of 3-hydroxypropionic acid and 1, 3-propanediol from glycerol using resting cells of the lactate dehydrogenase-deficient recombinant Klebsiella pneumoniae overexpressing an aldehyde dehydrogenase. Bioresour. Technol. 135, 555-563 (2013).
21. Kumar, V., et al. Co-production of 3-hydroxypropionic acid and 1, 3-propanediol from glycerol using resting cells of recombinant Klebsiella pneumoniae J2B strain overexpressing aldehyde dehydrogenase. Appl. Microbial. Biotechnol. 96(2), 373-383 (2012).
22. Cadieux, P., et al. Evaluation of reuterin production in urogenital probiotic Lactobacillus reuteri RC-14. Appl. Envir. Microbiol. 74, 4645-4649 (2008).
23. Murakami, K. S., Masuda, S., & Darst, S. A. Structural basis of transcription initiation: RNA polymerase holoenzyme at 4A resolution. Science 296(5571), 1280-1284 (2002).
24. Brown, L., Gentry, D., Elliott, T., & Cashel, M. DksA affects ppGpp induction of RpoS at a translational level. J. Bacteriol. 184(16), 4455-4465 (2002).
25. Arfman, N., Worrell, V., & Ingram, L. O. Use of the tac promoter and lacIq for the controlled expression of Zymomonas mobilis fermentative genes in Escherichia coli and Zymomonas mobilis. J. Bacteriol. 174(22), 7370-7378 (1992).
26. Ashok, S., Raj, S. M., Rathnasingh, C., & Park, S. Development of recombinant Klebsiella pneumoniae ? dhaT strain for the coproduction of 3-hydroxypropionic acid and 1, 3-propanediol from glycerol. Appl. Microbiol. Biotechnol. 90(4), 1253-1265 (2011).
27. Luo, L. H., et al. Stimulation of reductive glycerol metabolism by overexpression of an aldehyde dehydrogenase in a recombinant Klebsiella pneumoniae strain defective in the oxidative pathway. J. Ind. Microbiol. Biotechnol. 38(8), 991-999 (2011).
28. Andreeben, B., & Steinbuchel, A. Biosynthesis and biodegradation of 3-hydroxypropionate-containing polyesters. Appl. Environ. Microbiol. 76(15), 4919-4925 (2010).
29. Huang, Y., Li, Z., Shimizu, K., & Ye, Q. Co-production of 3-hydroxypropionic acid and 1, 3-propanediol by Klebseilla pneumoniae expressing aldH under microaerobic conditions. Bioresour. Technol. 128, 505-512 (2013).
30. Liao, Y. C., et al. An experimentally validated genome-scale metabolic reconstruction of Klebsiella pneumoniae MGH 78578, iYL1228. J. Bacteriol. 197(7), 1710-1717 (2011).
31. Schellenberger, J., et al. Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox v2.0. Nat. Protoc. 6(9), 1290-1307 (2011).