Constitutive trichloroethylene degradation led by tac promoter chromosomally integrated upstream of phenol hydroxylase genes of Ralstonia sp. KN1 and its nucleotide sequence analysis

Journal of Bioscience and Bioengineering

Volumn 89, Issue 1, 2000, Pages 47-54

  Nakamura, Kanji ^a   Ishida, Hiroaki ^a   Iizumi, Taro ^a  

^a KURITA WATER INDUSTRIES LTD   (Japan)

Author keywords

Constitutive degradation; Phenol hydroxylase; Ralstonia sp.; Trichloroethylene

Indexed keywords

BACTERIA; CHLORINE COMPOUNDS; CLONING; DNA SEQUENCES; ENZYMES; GENES;

TRICHLOROETHYLENE;

BIODEGRADATION;

PHENOL DERIVATIVE; TRICHLOROETHYLENE;

ARTICLE; DEGRADATION; ENZYME ACTIVITY; ENZYME REGULATION; ENZYME SYNTHESIS; GENE EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; RALSTONIA; SEQUENCE ANALYSIS;

RALSTONIA;

EID: 0033966288     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(00)88049-4     Document Type: Article

References (32)

1. 1. Goeptar, A. R., Commandeur, J. N. M., van Ommen, B., van Bladeren, P. J., and Vermeulen, N. P. E.: Metabolism and kinetics of trichloroethylene in relation to toxicity and carcino-genicity. Relevance of the mercapturic acid pathway. Chem. Res. Toxicol., 8, 3-21 (1995).
2. 2. Maltoni, C. and Lefimine, G.: Carcinogenicity bioassays of vinylchloride. I. Research plan and early results. Environ. Res., 7, 387-396 (1974).
3. 3. Tsien, H.-C., Brusseau, G. A., Hanson, R. S., and Wackett, L. P.: Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b. Appl. Environ. Microbiol., 55, 3155-3161 (1989).
4. 4. Vanderberg, L. A. and Perry, J. J.: Dehalogenation by Mycobacterium vaccae JOB-5: role of the propane monooxygenase. Can. J. Microbiol., 40, 169-172 (1994).
5. 5. Arciero, D., Vannelli, T., Logan, M., and Hooper, A. B.: Degradation of trichloroethylene by the ammonia-oxidizing bacterium Nitrosomonas eutropaea. Biochem. Biophys. Res. Commun., 159, 640-643 (1989).
6. 6. Wackett, L. P. and Gibson, D. T.: Degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with Pseudomonas putida F1. Appl. Environ. Microbiol., 54, 1703-1708 (1988).
7. 7. Shields, M. S., Montgomery, S. O., Chapman, P. J., Cuskey, S. M., and Pritchard, P. H.: Novel pathway of toluene catabolism in the trichloroethylene-degrading bacterium G4. Appl. Environ. Microbiol., 55, 1624-1629 (1989).
8. 8. Winter, R. B., Yen, K.-M., and Ensley, B. D.: Efficient degradation of trichloroethylene by a recombinant Escherichia coli. Bio/Technology, 7, 282-285 (1989).
9. 9. Fujita, M., Ike, M., Hioki, J., Kataoka, K., and Takeo, M.: Trichloroethylene degradation by genetically engineered bacteria carrying cloned phenol catabolic genes. J Ferment. Bioeng., 79, 100-106 (1995).
10. 10. Kim, Y., Ayoubi, P., and Harker, A. R.: Constitutive expression of the cloned phenol hydroxylase gene(s) from Alcaligenes eutrophus JMP134 and concomitant trichloroethylene oxidation. Appl. Environ. Microbiol., 62, 3227-3233 (1996).
11. 11. Nakamura, K. and Miyaji, Y.: Degradation of trichloroethylene by a genetically engineered microorganism containing a phenol hydroxylase gene. Proc. Environ. Eng. Res., 29, 17-27 (1992).
12. 12. Pflugmacher, U., Averhoff, B., and Gottschalk, G.: Cloning, sequencing, and expression of isopropylbenzene degradation genes from Pseudomonas sp. strain JP1: identification of isopropylbenzene dioxygenase that mediates trichloroethylene oxidation. Appl. Environ. Microbiol., 62, 3967-3977 (1996).
13. 13. Berendes, F., Sabarth, N., Averhoff, B., and Gottschalk, G.: Construction and use of an ipb DNA module to generate Pseudomonas strains with constitutive trichloroethene and isopropylbenzene oxidation activity. Appl. Environ. Microbiol., 64, 2454-2462 (1998).
14. 14. Shields, M. S. and Reagin, M. J.: Selection of a Pseudomonas cepacia strain constitutive for the degradation of trichloroethylene. Appl. Environ. Microbiol., 58, 3977-3983 (1992).
15. 15. Yee, D. C., Maynard, J. A., and Wood, T. K.: Rhizoremediation of trichloroethylene by a recombinant, root-colonizing Pseudomonas fluorescens strain expressing toluene ortho-monooxygenase constitutively. Appl. Environ. Microbiol., 64, 112-118 (1998).
16. 16. Nakamura, K., Shibata, M., and Miyaji, Y.: Substrate affinity of oligotrophic bacteria in biofilm reactors. Wat. Sci. Tech., 21, 779-790 (1989).
17. 17. Nakamura, K. and Ishida, H.: Development of a genetically engineered microorganism for trichloroethylene degradation by homologous recombination and analysis of its gene expression. Proc. Environ. Eng. Res., 33, 165-175 (1996).
18. 18. Maniatis, T., Fritsch, E. F., and Sambrook, J.: Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1982).
19. 19. Hanahan, D.: Techniques for transformation of Escherichia coli, p. 109-135. In Glover, D. M. (ed.), DNA cloning. A practical approach, vol. 1. IRL Press, Oxford (1985).
20. 20. Simon, R., Priefer, U., and Puhler, A.: A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology, 1, 748-791 (1983).
21. 21. Yanish-Perron, C., Vieira, J., and Messing, J.: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 33, 103-119 (1985).
22. 22. Amann, E., Ochs, B., and Abel K.-J.: Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene, 69, 301-315 (1988).
23. 23. Sanger, F., Nicklen, S., and Coulson, A. R.: DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA, 74, 5463-5467 (1977).
24. 24. Meighen, E.: Molecular biology of bacterial bioluminescence. Microbiol. Rev., 55, 123-142 (1991).
25. 25. Stanier, R. Y., Palleroni, N. J., and Duodoroff, M.: The aerobic Pseudomonads: a taxonomic study. J. Gen. Microbiol., 43, 159-271 (1966).
26. 26. Blouin, K., Walker, S. G., Smit, J., and Turner, R. F. B.: Characterization of in vivo reporter systems for gene expression and biosensor applications based on luxAB luciferase genes. Appl. Environ. Microbiol., 62, 2013-2021 (1996).
27. 27. Nordlund, I., Powlowski, J., and Shingler, V.: Complete nucleotide sequence and polypeptide analysis of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600. J. Bacteriol., 172, 6826-6833 (1990).
28. 28. Hino, S., Watanabe, K., and Takahashi, N.: Phenol hydroxylase cloned from Ralstonia eutropha strain E2 exhibits novel kinetic properties. Microbiology, 144, 1765-1772 (1998).
29. 29. Ayoubi, P. J. and Harker, A. R.: Whole-cell kinetics of trichloroethylene degradation by phenol hydroxylase in a Ralstonia eutropha JMP134 derivative. Appl. Environ. Microbiol., 64, 4353-4356 (1998).
30. 30. Harker, A. R. and Kim, Y.: Trichloroethylene degradation by two independent aromatic-degrading pathways in Alcaligenes eutrophus JMP134. Appl. Environ. Microbiol., 56, 1179-1181 (1990).
31. 31. Powlowski, J. and Shingler, V.: Genetics and biochemistry of phenol degradation by Pseudomonas sp. CF600. Biodegradation, 5, 219-236 (1994).
32. 32. Powlowski, J., Sealy, J., Shingler, V., and Cadieux, E.: On the role of DmpK, an auxiliary protein associated with multi-component phenol hydroxylase from Pseudomonas sp. CF600. J. Biol. Chem., 272, 945-951 (1997).