A new defective helper RNA to produce recombinant sindbis virus that infects neurons but does not propagate

Frontiers in Neuroanatomy

Volumn 10, Issue MAY, 2016, Pages

  Kebschull, Justus M ^a,b   da Silva, Pedro Garcia ^b,c   Zador, Anthony M ^b  

^a WATSON SCHOOL OF BIOLOGICAL SCIENCES   (United States)

^b Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

^c CHAMPALIMAUD CENTRE FOR THE UNKNOWN   (Portugal)

Author keywords

Defective helper RNAs; Neurotropism; Propagation competence; Sindbis virus; Viral tracing

Indexed keywords

DEFECTIVE HELPER RNA; RECOMBINANT RNA; STRUCTURAL PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; DNA PACKAGING; GENE EXPRESSION; HIPPOCAMPAL NEURONAL CULTURE; MALE; MOUSE; NEUROTROPISM; NONHUMAN; OPEN READING FRAME; PATHOGEN CLEARANCE; SECONDARY INFECTION; SINDBIS VIRUS; TRANSGENE; VIRUS INFECTION; VIRUS PARTICLE; VIRUS PLAQUE; VIRUS RECOMBINANT;

EID: 84971280857     PISSN: None     EISSN: 16625129     Source Type: Journal    
DOI: 10.3389/fnana.2016.00056     Document Type: Article

References (20)

1. Bredenbeek, P. J., Frolov, I., Rice, C. M., and Schlesinger, S. (1993). Sindbis virus expression vectors: packaging of RNA replicons by using defective helper RNAs. J. Virol. 67, 6439-6446.
2. Cetin, A., Komai, S., Eliava, M., Seeburg, P. H., and Osten, P. (2007). Stereotaxic gene delivery in the rodent brain. Nat. Protoc. 1, 3166-3173. doi: 10.1038/nprot. 2006.450
3. Ehrengruber, M. U. (2002a). Alphaviral gene transfer in neurobiology. Brain Res. Bull. 59, 13-22. doi: 10.1016/s0361-9230(02)00858-4
4. Ehrengruber, M. U. (2002b). Alphaviral vectors for gene transfer into neurons. Mol. Neurobiol. 26, 183-202. doi: 10.1385/mn: 26: 2-3: 183
5. Ehrengruber, M. U., Lundstrom, K., Schweitzer, C., Heuss, C., Schlesinger, S., and Gähwiler, B. H. (1999). Recombinant semliki forest virus and sindbis virus efficiently infect neurons in hippocampal slice cultures. Proc. Natl. Acad. Sci. U S A 96, 7041-7046. doi: 10.1073/pnas.96.12.7041
6. Frolova, E., Frolov, I., and Schlesinger, S. (1997). Packaging signals in alphaviruses. J. Virol. 71, 248-258.
7. Furuta, T., Tomioka, R., Taki, K., Nakamura, K., Tamamaki, N., and Kaneko, T. (2001). in vivo transduction of central neurons using recombinant Sindbis virus: golgi-like labeling of dendrites and axons with membrane-targeted fluorescent proteins. J. Histochem. Cytochem. 49, 1497-1508. doi: 10. 1177/002215540104901203
8. Geigenmüller-Gnirke, U., Weiss, B., Wright, R., and Schlesinger, S. (1991). Complementation between Sindbis viral RNAs produces infectious particles with a bipartite genome. Proc. Natl. Acad. Sci. U S A 88, 3253-3257. doi: 10. 1073/pnas.88.8.3253
9. Ghosh, S., Larson, S. D., Hefzi, H., Marnoy, Z., Cutforth, T., Dokka, K., et al. (2011). Sensory maps in the olfactory cortex defined by long-range viral tracing of single neurons. Nature 472, 217-220. doi: 10.1038/nature 09945
10. Hahn, C. S., Hahn, Y. S., Braciale, T. J., and Rice, C. M. (1992). Infectious Sindbis virus transient expression vectors for studying antigen processing and presentation. Proc. Natl. Acad. Sci. U S A 89, 2679-2683. doi: 10.1073/pnas.89. 7.2679
11. Hayashi, Y., Shi, S. H., Esteban, J. A., Piccini, A., Poncer, J. C., and Malinow, R. (2000). Driving AMPA receptors into synapses by LTP and CaMKII: requirement for GluR1 and PDZ domain interaction. Science 287, 2262-2267. doi: 10.1126/science.287.5461.2262
12. Kawamura, M., Namba, H., Otsu, Y., Hayashi, Y., Takei, N., and Nawa, H. (2003). Characterization of novel bicistronic sindbis virus vectors, SinEGdsp and SinIRES-EG, in cultured neurons. Recent Res. Dev. Neurochem. 6, 105-120.
13. Kim, J., Dittgen, T., Nimmerjahn, A., Waters, J., Pawlak, V., Helmchen, F., et al. (2004). Sindbis vector SINrep(nsP2S726): a tool for rapid heterologous expression with attenuated cytotoxicity in neurons. J. Neurosci. Methods 133, 81-90. doi: 10.1016/j.jneumeth.2003.09.029
14. Lustig, S., Jackson, A. C., Hahn, C. S., Griffin, D. E., Strauss, E. G., and Strauss, J. H. (1988). Molecular basis of Sindbis virus neurovirulence in mice. J. Virol. 62, 2329-2336.
15. Malinow, R., Hayashi, Y., Maletic-Savatic, M., Zaman, S. H., Poncer, J.-C., Shi, S.-H., et al. (2010). Introduction of green fluorescent protein (GFP) into hippocampal neurons through viral infection. Cold Spring Harbor Protoc. 2010: pdb.prot5406. doi: 10.1101/pdb.prot5406
16. Pak, D. T. S., Yang, S., Rudolph-Correia, S., Kim, E., and Sheng, M. (2001). Regulation of dendritic spine morphology by SPAR, a PSD-95-associated RapGAP. Neuron 31, 289-303. doi: 10.1016/s0896-6273(01) 00355-5
17. Shi, S., Hayashi, Y., Esteban, J. A., and Malinow, R. (2001). Subunit-specific rules governing AMPA receptor trafficking to synapses in hippocampal pyramidal neurons. Cell 105, 331-343. doi: 10.1016/s0092-8674(01) 00321-x
18. Strauss, E. G., Rice, C. M., and Strauss, J. H. (1984). Complete nucleotide sequence of the genomic RNA of Sindbis virus. Virology 133, 92-110. doi: 10.1016/0042-6822(84)90428-8
19. Taylor, A. M., Dieterich, D. C., Ito, H. T., Kim, S. A., and Schuman, E. M. (2010). Microfluidic local perfusion chambers for the visualization and manipulation of synapses. Neuron 66, 57-68. doi: 10.1016/j.neuron.2010. 03.022
20. Xiong, C., Levis, R., Shen, P., Schlesinger, S., Rice, C. M., and Huang, H. V. (1989). Sindbis virus: an efficient, broad host range vector for gene expression in animal cells. Science 243, 1188-1191. doi: 10.1126/science.29 22607