Sindbis vector SINrep(nsP2S726): A tool for rapid heterologous expression with attenuated cytotoxicity in neurons

Journal of Neuroscience Methods

Volumn 133, Issue 1-2, 2004, Pages 81-90

  Kim, Jinhyun ^a,c   Dittgen, Tanjew ^a   Nimmerjahn, Axel ^a   Waters, Jack ^a   Pawlak, Verena ^a   Helmchen, Fritjof ^a   Schlesinger, Sondra ^b   Seeburg, Peter H ^a   Osten, Pavel ^a  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Heterologous protein expression; In vitro; In vivo; Neuron; Sindbis virus

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; EVALUATION; FLUORESCENCE MICROSCOPY; GENE MUTATION; HETEROLOGOUS EXPRESSION; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; RAT; SINDBIS VIRUS; VIRUS STRAIN;

EID: 0742306850     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2003.09.029     Document Type: Article

References (19)

1. Agapov E.V., Frolov I., Lindenbach B.D., Pragai B.M., Schlesinger S., Rice C.M. Noncytopathic Sindbis virus RNA vectors for heterologous gene expression. Proc. Natl. Acad. Sci. U.S.A. 95:1998;12989-12994.
2. Bredenbeek P.J., Frolov I., Rice C.M., Schlesinger S. Sindbis virus expression vectors: packaging of RNA replicons by using defective helper RNAs. J. Virol. 67:1993;6439-6446.
3. Brewer G.J., Torricelli J.R., Evege E.K., Price P.J. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J. Neurosci. Res. 35:1993;567-576.
4. Dryga S.A., Dryga O.A., Schlesinger S. Identification of mutations in a Sindbis virus variant able to establish persistent infection in BHK cells: the importance of a mutation in the nsP2 gene. Virology. 228:1997;74-83.
5. Ehrengruber M.U., Lundstrom K., Schweitzer C., Heuss C., Schlesinger S., Gahwiler B.H. Recombinant Semliki Forest virus and Sindbis virus efficiently infect neurons in hippocampal slice cultures. Proc. Natl. Acad. Sci. U.S.A. 96:1999;7041-7046.
6. Ehrengruber M.U., Renggli M., Raineteau O., Hennou S., Vaha-Koskela M.J., Hinkkanen A.E.et al. Semliki Forest virus A7(74) transduces hippocampal neurons and glial cells in a temperature-dependent dual manner. J. Neurovirol. 9:2003;16-28.
7. Frolov I., Schlesinger S. Comparison of the effects of Sindbis virus and Sindbis virus replicons on host cell protein synthesis and cytopathogenicity in BHK cells. J. Virol. 68:1994;1721-1727.
8. Griffin D.E., Hardwick J.M. Regulators of apoptosis on the road to persistent alphavirus infection. Annu. Rev. Microbiol. 51:1997;565-592.
9. Kleinfeld D, Denk W. Two-photon imaging of neocortical microcirculation. In: Yuste R, Lanni F, Konnerth A, editors, Imaging neurons: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Press; 1999. p. 23.15-21.
10. Lendvai B., Stern E.A., Chen B., Svoboda K. Experience-dependent plasticity of dendritic spines in the developing rat barrel cortex in vivo. Nature. 404:2000;876-881.
11. Lewis J., Wesselingh S.L., Griffin D.E., Hardwick J.M. Alphavirus-induced apoptosis in mouse brains correlates with neurovirulence. J. Virol. 70:1996;1828-1835.
12. Lundstrom K., Abenavoli A., Malgaroli A., Ehrengruber M.U. Novel semliki forest virus vectors with reduced cytotoxicity and temperature sensitivity for long-term enhancement of transgene expression. Mol. Ther. 7:2003;202-209.
13. Lundstrom K., Rotmann D., Hermann D., Schneider E.M., Ehrengruber M.U. Novel mutant Semliki Forest virus vectors: gene expression and localization studies in neuronal cells. Histochem. Cell. Biol. 115:2001;83-91.
14. Lustig S., Jackson A.C., Hahn C.S., Griffin D.E., Strauss E.G., Strauss J.H. Molecular basis of Sindbis virus neurovirulence in mice. J. Virol. 62:1988;2329-2336.
15. 2+-permeable AMPA receptors at hippocampal CA1 synapses and induction of NMDA receptor-independent long-term potentiation. Eur. J. Neurosci. 13:2001;1635-1643.
16. Osten P., Khatri L., Perez J.L., Kohr G., Giese G., Daly C.et al. Mutagenesis reveals a role for ABP/GRIP binding to GluR2 in synaptic surface accumulation of the AMPA receptor. Neuron. 27:2000;313-325.
17. Perri S., Driver D.A., Gardner J.P., Sherrill S., Belli B.A., Dubensky T.W. Jr.et al. Replicon vectors derived from Sindbis virus and Semliki forest virus that establish persistent replication in host cells. J. Virol. 74:2000;9802-9807.
18. Schlesinger S., Dubensky T.W. Alphavirus vectors for gene expression and vaccines. Curr. Opin. Biotechnol. 10:1999;434-439.
19. Strauss J.H., Strauss E.G. The alphaviruses: gene expression, replication, and evolution. Microbiol. Rev. 58:1994;491-562.