Expression of Shiga toxin 2 (Stx2) in highly virulent Stx-producing Escherichia coli (STEC) carrying different anti-terminator (q) genes

Microbial Pathogenesis

Volumn 97, Issue , 2016, Pages 1-8

  Olavesen, Kristoffer K ^a   Lindstedt, Bjørn Arne ^b   Løbersli, Inger ^a   Brandal, Lin T ^a  

^a NORWEGIAN INSTITUTE OF PUBLIC HEALTH   (Norway)

^b NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

Author keywords

Anti terminator Q; Bacteriophage induction; Expression of stx2; Mitomycin C; Q gene; Shiga toxin 2; STEC

Indexed keywords

MESSENGER RNA; MITOMYCIN; SORBITOL; VEROTOXIN 2; VIRULENCE FACTOR; ANTITERMINATOR PROTEINS, BACTERIA; BACTERIAL DNA; BACTERIAL PROTEIN; RNA BINDING PROTEIN;

ARTICLE; BACTERIAL STRAIN; BACTERIOPHAGE; CONTROLLED STUDY; GENE; GENE EXPRESSION; HEMOLYTIC UREMIC SYNDROME; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NORWAY; PATHOGENICITY; PRIORITY JOURNAL; Q21 GENE; Q933 GENE; QO111 GENE; SEROTYPE; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; BIOSYNTHESIS; CLASSIFICATION; COLIPHAGE; GENE EXPRESSION PROFILING; GENETICS; GENOTYPE; PROPHAGE; VIRULENCE;

BACTERIAL PROTEINS; COLIPHAGES; DNA, BACTERIAL; GENE EXPRESSION PROFILING; GENOTYPE; MULTIPLEX POLYMERASE CHAIN REACTION; NORWAY; PROPHAGES; RNA-BINDING PROTEINS; SEROGROUP; SHIGA TOXIN 2; SHIGA-TOXIGENIC ESCHERICHIA COLI; VIRULENCE; VIRULENCE FACTORS;

EID: 84969913448     PISSN: 08824010     EISSN: 10961208     Source Type: Journal    
DOI: 10.1016/j.micpath.2016.05.010     Document Type: Article

References (45)

1. Steyert S.R., et al. Comparative genomics and stx phage characterization of LEE-negative Shiga toxin-producing Escherichia coli. Front. Cell Infect. Microbiol. 2012, 2:133.
2. Friedrich A.W., et al. Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. J. Infect. Dis. 2002, 185(1):74-84.
3. Ethelberg S., et al. Virulence factors for hemolytic uremic syndrome, Denmark. Emerg. Infect. Dis. 2004, 10(5):842-847.
4. Persson S., et al. Subtyping method for Escherichia coli shiga toxin (verocytotoxin) 2 variants and correlations to clinical manifestations. J. Clin. Microbiol. 2007, 45(6):2020-2024.
5. Mellmann A., et al. Analysis of collection of hemolytic uremic syndrome-associated enterohemorrhagic Escherichia coli. Emerg. Infect. Dis. 2008, 14(8):1287-1290.
6. Fuller C.A., et al. Shiga toxin subtypes display dramatic differences in potency. Infect. Immun. 2011, 79(3):1329-1337.
7. Messens W., et al. Defining pathogenic verocytotoxin-producing Escherichia coli (VTEC) from cases of human infection in the European Union, 2007-2010. Epidemiol. Infect. 2015, 143(8):1652-1661.
8. de Sablet T., et al. Differential expression of stx2 variants in Shiga toxin-producing Escherichia coli belonging to seropathotypes A and C. Microbiology 2008, 154(Pt 1):176-186.
9. Shringi S., et al. Differential virulence of clinical and bovine-biased enterohemorrhagic Escherichia coli O157:H7 genotypes in piglet and Dutch belted rabbit models. Infect. Immun. 2012, 80(1):369-380.
10. Mellor G.E., et al. Geographically distinct Escherichia coli O157 isolates differ by lineage, Shiga toxin genotype, and total shiga toxin production. J. Clin. Microbiol. 2015, 53(2):579-586.
11. Schmidt H. Shiga-toxin-converting bacteriophages. Res. Microbiol. 2001, 152(8):687-695.
12. Calderwood S.B., et al. Nucleotide sequence of the Shiga-like toxin genes of Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 1987, 84(13):4364-4368.
13. Wagner P.L., et al. Role for a phage promoter in Shiga toxin 2 expression from a pathogenic Escherichia coli strain. J. Bacteriol. 2001, 183(6):2081-2085.
14. Haugum K., et al. Identification of the anti-terminator qO111:H)- gene in Norwegian sorbitol-fermenting Escherichia coli O157:NM. FEMS Microbiol. Lett. 2012, 329(2):102-110.
15. Brussow H., Canchaya C., Hardt W.D. Phages and the evolution of bacterial pathogens: from genomic rearrangements to lysogenic conversion. Microbiol. Mol. Biol. Rev. 2004, 68(3):560-602. table of contents.
16. Matsumoto M., et al. Identification and epidemiological description of enterohemorrhagic Escherichia coli O157 strains producing low amounts of Shiga toxin 2 in Aichi Prefecture, Japan. Jpn. J. Infect. Dis. 2008, 61(6):442-445.
17. Koitabashi T., et al. Genetic characterization of Escherichia coli O157: H7/- strains carrying the stx2 gene but not producing Shiga toxin 2. Microbiol. Immunol. 2006, 50(2):135-148.
18. Lejeune J.T., et al. Human Escherichia coli O157:H7 genetic marker in isolates of bovine origin. Emerg. Infect. Dis. 2004, 10(8):1482-1485.
19. Karmali M.A., et al. Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease. J. Clin. Microbiol. 2003, 41(11):4930-4940.
20. Frank C., et al. Epidemic profile of Shiga-toxin-producing Escherichia coli O104:H4 outbreak in Germany. N. Engl. J. Med. 2011, 365(19):1771-1780.
21. EFSA Panel on Biological Hazards (BIOHAZ); Scientific Opinion on VTEC-seropathotype and scientific criteria regarding pathogenicity assessment. EFSA J. 2013, 11(4):3138.
22. Schimmer B., et al. Outbreak of haemolytic uraemic syndrome in Norway caused by stx2-positive Escherichia coli O103:H25 traced to cured mutton sausages. BMC Infect. Dis. 2008, 8:41.
23. Nygård K.V.L., Heier B.T., Bruun T., Kapperud G. Annual Report: Foodborne Infections and Outbreaks in 2009 2010, Reporting system for infectious diseases (MSIS) and web-based system for outbreak warning (Vesuv)., In Norwegian. http://www.fhi.no/eway/default.aspx?pid=239&trg=Content_6466&Main_6157=6263:0:25,%206493&MainContent_6263=6466:0:25,%206494&Content_6466=6259:84108::0:6184:2:::0:0.
24. O'Brien A.O., et al. Escherichia coli O157:H7 strains associated with haemorrhagic colitis in the United States produce a Shigella dysenteriae 1 (SHIGA) like cytotoxin. Lancet 1983, 1(8326 Pt 1):702.
25. Neupane M., et al. Shiga toxin 2 overexpression in Escherichia coli O157:H7 strains associated with severe human disease. Microb. Pathog. 2011, 51(6):466-470.
26. Kawano K., et al. Relationship between stx genotype and Stx2 expression level in Shiga toxin-producing Escherichia coli O157 strains. Jpn. J. Infect. Dis. 2012, 65(4):322-325.
27. Brandal L.T., et al. Shiga toxin-producing escherichia coli infections in Norway, 1992-2012: characterization of isolates and identification of risk factors for haemolytic uremic syndrome. BMC Infect. Dis. 2015, 15(1):324.
28. Boisen N., et al. New adhesin of enteroaggregative Escherichia coli related to the Afa/Dr/AAF family. Infect. Immun. 2008, 76(7):3281-3292.
29. Unkmeir A., Schmidt H. Structural analysis of phage-borne stx genes and their flanking sequences in shiga toxin-producing Escherichia coli and Shigella dysenteriae type 1 strains. Infect. Immun. 2000, 68(9):4856-4864.
30. Pavlovic M., et al. Development of a multiplex real-time polymerase chain reaction for simultaneous detection of enterohemorrhagic Escherichia coli and enteropathogenic Escherichia coli strains. Foodborne Pathog. Dis. 2010, 7(7):801-808.
31. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001, 25(4):402-408.
32. Muniesa M., et al. Shiga toxin 2-converting bacteriophages associated with clonal variability in Escherichia coli O157:H7 strains of human origin isolated from a single outbreak. Infect. Immun. 2003, 71(8):4554-4562.
33. Tyler J.S., Mills M.J., Friedman D.I. The operator and early promoter region of the Shiga toxin type 2-encoding bacteriophage 933W and control of toxin expression. J. Bacteriol. 2004, 186(22):7670-7679.
34. Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001, 29(9). e45.
35. Schmittgen T.D., Livak K.J. Analyzing real-time PCR data by the comparative C(T) method. Nat. Protoc. 2008, 3(6):1101-1108.
36. Muniesa M., et al. Diversity of stx2 converting bacteriophages induced from Shiga-toxin-producing Escherichia coli strains isolated from cattle. Microbiology 2004, 150(Pt 9):2959-2971.
37. Smith D.L., et al. Comparative genomics of Shiga toxin encoding bacteriophages. BMC Genomics 2012, 13:311.
38. Yin S., et al. Escherichia coli O157:H7 strains harbor at least three distinct sequence types of Shiga toxin 2a-converting phages. BMC Genomics 2015, 16(1):733.
39. Laing C.R., et al. A comparison of Shiga-toxin 2 bacteriophage from classical enterohemorrhagic Escherichia coli serotypes and the German E. coli O104:H4 outbreak strain. PLoS One 2012, 7(5):e37362.
40. Rosser T., et al. Pathogenic potential of emergent sorbitol-fermenting Escherichia coli O157:NM. Infect. Immun. 2008, 76(12):5598-5607.
41. Beutin L., et al. Spread of a distinct Stx2-encoding phage prototype among Escherichia coli O104:H4 strains from outbreaks in Germany, Norway, and Georgia. J. Virol. 2012, 86(19):10444-10455.
42. Boisen N., et al. Shiga toxin 2a and Enteroaggregative Escherichia coli - a deadly combination. Gut Microbes 2015, 1-7.
43. Iversen H., et al. The gut bacterium Bacteroides thetaiotaomicron influences the virulence potential of the enterohemorrhagic Escherichia coli O103:H25. PLoS One 2015, 10(2). e0118140.
44. Iversen H., et al. Commensal E. coli Stx2 lysogens produce high levels of phages after spontaneous prophage induction. Front. Cell Infect. Microbiol. 2015, 5:5.
45. L'Abee-Lund T.M., et al. The highly virulent 2006 Norwegian EHEC O103:H25 outbreak strain is related to the 2011 German O104:H4 outbreak strain. PLoS One 2012, 7(3). e31413.