RNA Extraction from a Mycobacterium under Ultrahigh Electric Field Intensity in a Microfluidic Device

Analytical Chemistry

Volumn 88, Issue 10, 2016, Pages 5053-5057

  Ma, Sai ^a   Bryson, Bryan D ^b   Sun, Chen ^a   Fortune, Sarah M ^b   Lu, Chang ^a  

^a State University   (United States)

^b HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; ELECTRIC FIELDS; EXTRACTION; GENE EXPRESSION; MICROFLUIDICS; NUCLEIC ACIDS; PACKED BEDS; POLYMERASE CHAIN REACTION; RNA; TRANSCRIPTION;

EFFECTIVE APPROACHES; EXTRACTION EFFICIENCIES; INTRACELLULAR MATERIALS; MICRO-FLUIDIC DEVICES; MOLECULAR ASSAYS; REVERSE TRANSCRIPTION; STATE-OF-THE-ART METHODS; ULTRA-HIGH ELECTRIC FIELDS;

CYTOLOGY;

RNA; SILICON DIOXIDE;

CHEMISTRY; DEVICES; ELECTROMAGNETISM; GENETICS; ISOLATION AND PURIFICATION; LAB ON A CHIP; MICROFLUIDIC ANALYSIS; MYCOBACTERIUM SMEGMATIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ELECTROMAGNETIC FIELDS; LAB-ON-A-CHIP DEVICES; MICROFLUIDIC ANALYTICAL TECHNIQUES; MYCOBACTERIUM SMEGMATIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; SILICON DIOXIDE;

EID: 84969871609     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/acs.analchem.6b00381     Document Type: Article

References (31)

1. Wang, Z.; Gerstein, M.; Snyder, M. Nat. Rev. Genet. 2009, 10, 57-63 10.1038/nrg2484
2. Schmittgen, T. D.; Livak, K. J. Nat. Protoc. 2008, 3, 1101-1108 10.1038/nprot.2008.73
3. Sealfon, S. C.; Chu, T. T. Methods Mol. Biol. 2011, 671, 3-34 10.1007/978-1-59745-551-0-1
4. Mortazavi, A.; Williams, B. A.; McCue, K.; Schaeffer, L.; Wold, B. Nat. Methods 2008, 5, 621-628 10.1038/nmeth.1226
5. Croucher, N. J.; Thomson, N. R. Curr. Opin. Microbiol. 2010, 13, 619-624 10.1016/j.mib.2010.09.009
6. Fleige, S.; Pfaffl, M. W. Mol. Aspects Med. 2006, 27, 126-139 10.1016/j.mam.2005.12.003
7. Imbeaud, S.; Graudens, E.; Boulanger, V.; Barlet, X.; Zaborski, P.; Eveno, E.; Mueller, O.; Schroeder, A.; Auffray, C. Nucleic Acids Res. 2005, 33 e56 10.1093/nar/gni054
8. Hurley, S. S.; Splitter, G.; Welch, R. J. Clin. Microbiol. 1987, 25, 2227-2229
9. Rustad, T.; Roberts, D.; Liao, R.; Sherman, D. In Mycobacteria Protocols; Parish, T.; Brown, A. C., Eds.; Humana Press: Totowa, NJ, 2009; pp 13-22.
10. Rio, D. C.; Ares, M.; Hannon, G. J.; Nilsen, T. W. Cold Spring Harb. Protoc. 2010, 2010, pdb.prot5439 10.1101/pdb.prot5439
11. Mangan, J. A.; Sole, K. M.; Mitchison, D. A.; Butcher, P. D. Nucleic Acids Res. 1997, 25, 675-676
12. Aldous, W. K.; Pounder, J. I.; Cloud, J. L.; Woods, G. L. J. Clin. Microbiol. 2005, 43, 2471-2473 10.1128/JCM.43.5.2471-2473.2005
13. Jahn, C. E.; Charkowski, A. O.; Willis, D. K. J. Microbiol. Methods 2008, 75, 318-324 10.1016/j.mimet.2008.07.004
14. Vandeventer, P. E.; Weigel, K. M.; Salazar, J.; Erwin, B.; Irvine, B.; Doebler, R.; Nadim, A.; Cangelosi, G. A.; Niemz, A. J. Clin. Microbiol. 2011, 49, 2533-2539 10.1128/JCM.02171-10
15. Leite, G. M.; Magan, N.; Medina, A. J. Microbiol. Methods 2012, 88, 413-418 10.1016/j.mimet.2012.01.011
16. Stahl, D. A.; Urbance, J. W. J. Bacteriol. 1990, 172, 116-124
17. Geng, T.; Lu, C. Lab Chip 2013, 13, 3803-3821 10.1039/C3LC50566A
18. Wang, H.-Y.; Bhunia, A. K.; Lu, C. Biosens. Bioelectron. 2006, 22, 582-588 10.1016/j.bios.2006.01.032
19. Bao, N.; Lu, C. Appl. Phys. Lett. 2008, 92, 214103 10.1063/1.2937088
20. Vulto, P.; Dame, G.; Maier, U.; Makohliso, S.; Podszun, S.; Zahn, P.; Urban, G. A. Lab Chip 2010, 10, 610-616 10.1039/B913481F
21. Geng, T.; Bao, N.; Sriranganathanw, N.; Li, L.; Lu, C. Anal. Chem. 2012, 84, 9632-9639 10.1021/ac3026064
22. Kim, J.; Johnson, M.; Hill, P.; Gale, B. K. Integr. Biol. 2009, 1, 574-586 10.1039/b905844c
23. Unger, M. A.; Chou, H.-P.; Thorsen, T.; Scherer, A.; Quake, S. R. Science 2000, 288, 113-116 10.1126/science.288.5463.113
24. Ma, S.; Loufakis, D. N.; Cao, Z.; Chang, Y.; Achenie, L. E. K.; Lu, C. Lab Chip 2014, 14, 2905-2909 10.1039/C4LC00498A
25. Ma, S.; Schroeder, B.; Sun, C.; Loufakis, D. N.; Cao, Z.; Sriranganathan, N.; Lu, C. Integr. Biol. 2014, 6, 973-978 10.1039/C4IB00172A
26. Sun, C.; Ouyang, M.; Cao, Z.; Ma, S.; Alqublan, H.; Sriranganathan, N.; Wang, Y.; Lu, C. Chem. Commun. 2014, 50, 11536-11539 10.1039/C4CC04730C
27. Sun, C.; Cao, Z.; Wu, M.; Lu, C. Anal. Chem. 2014, 86, 11403-11409 10.1021/ac503363m
28. Loufakis, D. N.; Cao, Z.; Ma, S.; Mittelman, D.; Lu, C. Chem. Sci. 2014, 5, 3331-3337 10.1039/C4SC00319E
29. Bao, N.; Wang, J.; Lu, C. Electrophoresis 2008, 29, 2939-2944 10.1002/elps.200700856
30. Geng, T.; Zhan, Y.; Wang, J.; Lu, C. Nat. Protoc. 2011, 6, 1192-1208 10.1038/nprot.2011.360
31. Wong, M. L.; Medrano, J. F. BioTechniques 2005, 39, 75-85 10.2144/05391RV01