Phyllanthin inhibits CCl4-mediated oxidative stress and hepatic fibrosis by down-regulating TNF-α/NF-κB, and pro-fibrotic factor TGF-β1 mediating inflammatory signaling

Toxicology and Industrial Health

Volumn 32, Issue 5, 2016, Pages 953-960

  Krithika, Rajesh ^a   Jyothilakshmi, Vasavan ^b   Verma, Ramtej Jayaram ^a  

^a GUJARAT UNIVERSITY   (India)

^b UNIVERSITY OF MADRAS   (India)

Author keywords

Liver fibrosis; LPO; NF B; phyllanthin; TGF 1; TNF

Indexed keywords

ALANINE AMINOTRANSFERASE; ANTIFIBROTIC AGENT; ASPARTATE AMINOTRANSFERASE; CARBON TETRACHLORIDE; COLLAGEN; FIBRONECTIN; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; LIVER PROTECTIVE AGENT; PHYLLANTHIN; TRANSFORMING GROWTH FACTOR BETA1; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG; ANTIOXIDANT; LIGNAN; MESSENGER RNA; PLANT EXTRACT; PROTECTIVE AGENT; TUMOR NECROSIS FACTOR;

ALANINE AMINOTRANSFERASE BLOOD LEVEL; ALCOHOL INTOXICATION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIOXIDANT ACTIVITY; ARTICLE; ASPARTATE AMINOTRANSFERASE BLOOD LEVEL; BLOOD SAMPLING; CARBON TETRACHLORIDE-INDUCED LIVER FIBROSIS; COMPARATIVE STUDY; CONTROLLED STUDY; DOWN REGULATION; DRUG EFFICACY; DRUG MECHANISM; DRUG POTENCY; ENZYME ACTIVITY; EXTRACELLULAR MATRIX; FEMALE; HEPG2 CELL LINE; IMMUNOBLOTTING; IN VIVO STUDY; LIPID PEROXIDATION; MOUSE; NONHUMAN; OUTCOME ASSESSMENT; OXIDATIVE STRESS; PHYLLANTHUS AMARUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; ANIMAL; CHEMICAL AND DRUG INDUCED LIVER INJURY; CHEMICALLY INDUCED; CHEMISTRY; CYTOLOGY; DRUG EFFECTS; GENETICS; HEP-G2 CELL LINE; HUMAN; LIVER; LIVER CIRRHOSIS; METABOLISM; PHYLLANTHUS;

ANIMALS; ANTIOXIDANTS; CARBON TETRACHLORIDE; CHEMICAL AND DRUG INDUCED LIVER INJURY; DOWN-REGULATION; FEMALE; HEP G2 CELLS; HUMANS; LIGNANS; LIPID PEROXIDATION; LIVER; LIVER CIRRHOSIS; MICE; NF-KAPPA B; OXIDATIVE STRESS; PHYLLANTHUS; PLANT EXTRACTS; PROTECTIVE AGENTS; RNA, MESSENGER; SIGNAL TRANSDUCTION; TRANSFORMING GROWTH FACTOR BETA1; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84968835258     PISSN: 07482337     EISSN: 14770393     Source Type: Journal    
DOI: 10.1177/0748233714532996     Document Type: Article

References (36)

1. Benyon RC, Arthur MJ, (2001) Extracellular matrix degradation and the role of hepatic stellate cells. Seminars in Liver Disease 21 (3): 373-384.
2. Boll M, Weber LW, Becker E, et al. (2001) Mechanism of carbon tetrachloride induced hepatotoxicity. Hepatocellular damage by reactive carbon tetrachloride metabolites. Zeitschrift fur Naturforsch [C] 56 (7/8): 649-659.
3. Calixto JB, Santos ARS, Filho VC, et al. (1998) A review of the plants of the Genus Phyllanthus: their chemistry, pharmacology, and therapeutic potential. Medical Research Review 18 (4): 225-258.
4. Chen A, Zhang L, Xu J, et al. (2002) The antioxidant (-)-epigallocatechin-3-gallate inhibits activated hepatic stellate cell growth and suppresses acetaldehyde-induced gene expression. Biochemical Journal 368 (3): 695-704.
5. Chirdchupunseree H, Pramyothin P, (2010) Protective activity of phyllanthin in ethanol-treated primary culture of rat hepatocytes. Journal of Ethnopharmacology 128 (1): 172-176.
6. Constandinou C, Henderson N, Iredale JP, (2005) Modeling liver fibrosis in rodents. Fibrosis research: methods and protocols. Methods in Molecular Medicine 117: 237-250.
7. Friedman SL, (1999) Cytokines and fibrogenesis. Seminars in Liver Disease 19 (2): 129-140.
8. Friedman SL, (2000) Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury. The Journal of Biological Chemistry 275 (4): 2247-2250.
9. Ghosh AK, (2002) Factors involved in type I collagen gene expression: implication in scleroderma. Experimental Biology and Medicine 227 (5): 301-314.
10. Gorski K, Cameiro M, Schibler U, (1986) Tissue-specific in vitro transcription from the mouse albumin promoter. Cell 47 (5): 767-776.
11. Heiss E, Herhaus C, Klimo K, et al. (2001) Nuclear factor-(kappa) B is a molecular target for sulforaphane-mediated anti-inflammatory mechanisms. The Journal of Biological Chemistry 276 (34): 32008-32015.
12. Jiang Y, Liu J, Waalkes M, et al. (2004) Changes in the gene expression associated with carbon tetrachloride-induced liver fibrosis persist after cessation of dosing in mice. Toxicological Sciences 79 (2): 404-410.
13. Ki RM, Goo MJ, Park JK, et al. (2010) Helicobacter pylori accelerates hepatic fibrosis by sensitizing transforming growth factor-β1-induced inflammatory signaling. Laboratory Investigations 90 (10): 1507-1516.
14. Kimura K, Ando K, Ohnishi H, et al. (1999) Immunopathogenesis of hepatic fibrosis in chronic liver injury induced by repeatedly administered concanavalin A. International Immunology 11 (9): 1491-1500.
15. Krithika R, Verma RJ, (2009a) Mitigation of carbon tetrachloride-induced damage by Phyllanthus amarus in liver of mice. Acta Poloniae Pharmaceutica Drug Research 66 (4): 439-445.
16. Krithika R, Verma RJ, (2009b) Ameliorative potential of Phyllanthus amarus against carbon tetrachloride induced hepatotoxicity. Acta Poloniae Pharmaceutica Drug Research 66 (5): 579-583.
17. 4-induced toxicity in HepG2 cell line. Chemico- Biological Interactions 181 (3): 351-358.
18. Krithika R, Verma RJ, Shrivastav PS, et al. (2011) Phyllanthin of standardized P. amarus extract attenuates liver oxidative stress and exerts cytoprotective activity on human hepatoma cell line. Journal of Clinical and Experimental Hepatology 1 (2): 57-67.
19. Liu S-Q, Yu J-P, Chen H-L, et al. (2006) Therapeutic effects and molecular mechanisms of Ginkgo biloba extract on liver fibrosis in rats. American Journal of Chinese Medicine 34 (1): 99-114.
20. Lotersztajn S, Julien B, Teixeira-Clerc F, et al. (2005) Hepatic fibrosis: molecular mechanisms and drug targets. Annual Reviews of Pharmacology and Toxicology 45: 605-628.
21. Lowry OH, Rosebrough NJ, Farr AL, et al. (1951) Protein measurement with folin-phenol reagent. The Journal of Biological Chemistry 193 (1): 265-275.
22. Man KNM, Philipsen S, Tan-Un KC, (2008) Localization and expression pattern of cytoglobin in carbon tetrachloride-induced liver fibrosis. Toxicological Letters 183 (1-3): 36-44.
23. Mann DA, Smart DE, (2002) Transcriptional regulation of hepatic stellate cell activation. Gut 50: 891-896.
24. Ohkawa H, Ohishi N, Yagi K, (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analatical Biochemistry 95 (2): 351-358.
25. Paradis V, Dargere D, Bonvoust F, et al. (2002) Effects and regulation of connective tissue growth factor on hepatic stellate cells. Laboratory Investigations 82 (6): 767-774.
26. Poli G, (2000) Pathogenesis of liver fibrosis: role of oxidative stress. Molecular Aspects of Medicine 21 (3): 49-98.
27. Saile B, Matthes N, El Armouche H, et al. (2001) The bcl, NFkB and p53/p21WAF1 systems are involved in spontaneous apoptosis and in the anti-apoptotic effect of TGF-β or TNF-α on activated hepatic stellate cells. European Journal of Cell Biology 80 (8): 554-561.
28. Sharma A, Singh RT, Handa SS, (1993) Estimation of phyllanthin and hypophyllanthin by high performance liquid chromatography in Phyllanthus amarus. Phytochemical Analysis 4 (5): 226-229.
29. Smith GP, (2012) Animal models of cutaneous and hepatic fibrosis. Progress in Molecular biology and Translational Sciences 105: 371-409.
30. Syamasundar KV, Singh B, Thakur RS, et al. (1985) Antihepatoprotective principles of Phyllanthus niruri herbs. Journal of Ethnopharmacology 14 (1): 41-44.
31. Tripathi AK, Verma RK, Gupta AK, et al. (2006) Quantitative determination of phyllanthin and hypophyllanthin in Phyllanthus species by high- performance thin layer chromatography. Phytochemical Analysis 17 (6): 394-397.
32. Venkatesan P, Satyan KS, Kumar MS, et al. (2003) Protective effect of aqueous extract of Phyllanthus amarus Linn., phyllanthin and nirocil against carbon tetrachloride-induced liver and brain toxicity. Indian Journal Pharmaceutical Sciences 65 (3): 309-312.
33. Verrecchia F, Mauvie A, (2004) TGF-β and TNF-α: antagonistic cytokines controlling type I collagen gene expression. Cellular Signaling 16 (8): 873-880.
34. Woessner JF, (1976) Determination of hydroxyproline in connective tissues. In: Hall DA, (ed) The Methodology of Connective Tissue Research. Oxford, UK: Joynson-Bruvvers Ltd, pp. 235-245.
35. Xu J, Fu Y, Chen A, (2003) Activation of peroxisome proliferator-activated receptor-gamma contributes to the inhibitory effects of curcumin on rat hepatic stellate cell growth. American Journal of Physiology Gastrointestinal and Liver Physiology 285 (1): G20-G30.
36. Zhang LJ, Yu JP, Li D, et al. (2004) Effects of cytokines on carbon tetrachloride-induced hepatic fibrogenesis in rats. World Journal of Gastroenterology 10 (1): 77-81.