Isolation, characterization and antioxidative effect of phyllanthin against CCl4-induced toxicity in HepG2 cell line

Chemico-Biological Interactions

Volumn 181, Issue 3, 2009, Pages 351-358

  Krithika, Rajesh ^a   Mohankumar, Ramasamy ^b   Verma, Ramtej J ^a   Shrivastav, Pranav S ^a   Mohamad, Illiyas L ^c   Gunasekaran, Palani ^c   Narasimhan, Srinivasan ^b  

^a GUJARAT UNIVERSITY   (India)

^b Asthagiri Herbal Research Foundation   (India)

^c Institute of Preventive Medicine   (India)

Author keywords

Antioxidative; Characterization; HepG2 cell line; Isolation; Phyllanthin; Phyllanthus amarus

Indexed keywords

ALANINE AMINOTRANSFERASE; ANTIOXIDANT; CARBON TETRACHLORIDE; GLUTATHIONE; LACTATE DEHYDROGENASE; PHYLLANTHIN; PHYLLANTHUS AMARUS EXTRACT; PLANT MEDICINAL PRODUCT; UNCLASSIFIED DRUG;

ANTIOXIDANT ACTIVITY; ARTICLE; CELL PROTECTION; CELL STRAIN HEPG2; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG STRUCTURE; HUMAN; HUMAN CELL; ISOLATION AND PURIFICATION; LIPID PEROXIDATION; LIVER PROTECTION;

ANTIOXIDANTS; CARBON TETRACHLORIDE POISONING; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, THIN LAYER; DRUG-INDUCED LIVER INJURY; HUMANS; LIGNANS; LIPID PEROXIDATION; MAGNETIC RESONANCE SPECTROSCOPY; SPECTROPHOTOMETRY, ULTRAVIOLET; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

PHYLLANTHUS AMARUS;

EID: 70249085883     PISSN: 00092797     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbi.2009.06.014     Document Type: Article

References (46)

1. Bhattacharjee R., and Sil P.C. Protein isolates from the herb, Phyllanthus niruri L. (Euphorbiaceae), plays hepatoprotective role against carbon tetrachloride induced liver damage via its antioxidant properties. Food Chem. Toxicol. 45 (2007) 817-826
2. Sane R.T., Chawla J.L., and Kuber V.V. Studies on Phyllanthus amarus. Indian Drugs-Part II 34 (1997) 654-655
3. Qian-Cutrone J., Huang S., Trimble J., Li H., Lin P.F., Alam M., Klohr S.E., and Kadow K.F. Niruside, a new HIV REV/RRE binding inhibitor from Phyllanthus niruri. J. Nat. Prod. 59 (1996) 196-199
4. Wongnawa M., Thaina P., Bumrungwong N., Nitiruangjarat A., Muso A., and Prasartthong V. Effect of Phyllanthus amarus Schum & Thonn. and its protective mechanism on paracetomol hepatotoxicity in rats. Acta Hort. 680 (2005) 195-201
5. 4-induced liver dysfunction. Curr. Sci. 68 (1995) 1243-1246
6. Harish R., and Shivanandappa T. Antioxidant activity and hepatoprotective potential of Phyllanthus niruri. Food Chem. 95 (2006) 180-185
7. Pramyothin P., Ngamtin C., Poungshompoo S., and Chaichantipyuth C. Hepatoprotective activity of Phyllanthus amarus Schum. et. Thonn. extract in ethanol treated rats: in vitro and in vivo studies. J. Ethnopharmacol. 114 (2007) 169-173
8. 1-induced liver damage in Mice. J. Ethnopharmacol. 113 (2007) 503-509
9. N. Kongstan, Effect of Phyllanthus amarus on hepatotoxicity induced by galactosamine in rats, Master's Thesis, Department of Toxicology, Faculty of Science, Mahidol University, 2000.
10. Raphael K.R., and Kuttan R. Inhibition of experimental gastric lesion and inflammation by Phyllanthus amarus extract. J. Ethnopharmacol. 87 (2003) 193-197
11. Okafor P.C., Ikpi M.E., Uwah I.E., Ebenso E.E., Ekpe U.J., and Umoren S.A. Inhibitory action of Phyllanthus amarus extracts on the corrosion of mild steel in acidic media. Corros. Sci. 50 (2008) 2310-2317
12. Thyagarajan S.P. Effects of an extracts from Phyllanthus niruri on hepatitis B and woodchuck hepatitis virus: in vitro and in vivo studies. Proc. Natl. Acad. Sci. USA 84 (1987) 274-288
13. 4-induced hepatotoxicity in Rattus norvegicus. Phytomedicine 15 (2008) 1053-1061
14. Shamasundar K.V., Singh B., Thakur R.S., Hussain A., Kiso Y., and Hikino H. Antihepatoprotective principles of Phyllanthus niruri herbs. J. Ethnopharmacol. 14 (1985) 41-44
15. Krishnamurti G.V., and Seshadri T.R. The bitter principle of Phylanthus niruri. Proc. Indian Acad. Sci. 24 (1946) 357-364
16. Row L.R., and Satyanarayana P. Crystalline constituents of euphorbiaceae-VI: the synthesis and absolute configuration of phyllanthin. Tetrahedron 23 (1967) 1915-1918
17. Row L.R., Srinivasulu C., Smith M., and Subsa Rao G.S.R. Crystalline constituents of Euphorbiaceae-V new lignans from Phyllanthus Niruri Linn-the constitution of phyllanthin. Tetrahedron 22 (1966) 2899-2908
18. Anjaneyulu A.S.R., Rao K.J., Row L.R., and Subrahmanyam C. Crystalline constituents of Euphorbiaceae-XII, isolation and structural elucidation of three new lignans from the leaves of Phyllanthus Niruri Linn. Tetrahedron 29 (1973) 1291-1298
19. 13C-NMR assignments of phyllanthin and hypophyllanthin: lignans that enhance cytotoxic responses with cultured multi-drug resistant cells. J. Nat. Prod. 56 (1993) 233-239
20. Murugaiyah V., and Chan K.L. Determination of four lignans in Phyllanthus niruri L. by a simple high-performance liquid chromatography method with fluorescence detection. J. Chromatogr. A 1154 (2007) 198-204
21. Willfor S.M., Smeds A.I., and Holmbom B.R. Chromatographic analysis of lignans. J. Chromatogr. A 1112 (2005) 64-77
22. Murugaiyah V., and Chan K.L. Analysis of lignans from Phyllanthus niruri L. in plasma using a simple HPLC method with fluorescence detection and its application in a pharmacokinetic study. J. Chromatogr. B 852 (2007) 138-144
23. Boll M., Weber L.W., Becker E., and Stampfl A. Mechanism of carbon tetrachloride-induced hepatotoxicity. Hepatocellular damage by reactive carbon tetrachloride metabolites. Z. Naturforsch. [C] 56 (2001) 649-659
24. Weddle C.C., Hornbrook K.R., and McCay P.B. Lipid peroxidation and alteration of membrane lipids in isolated hepatocytes exposed to carbon tetrachloride. J. Biol. Chem. 251 (1976) 4973-4978
25. Sundararajan R., Haja N.A., Venkatesan K., Mukherjee K., Saha B.P., Bandyopadhyay A., and Mukherjee P.K. Cystisus scoparius-a natural antioxidant. BMC Complement. Altern. Med. 6 (2006) 8
26. Ko K.M., Yick K.P., and Chiu T.W. Impaired hepatic antioxidant status in carbon tetrachloride intoxicated rats an in vivo model for screening herbal extracts with antioxidant activities. Fitoterapia 64 (1993) 539-544
27. Kim S.Y., Kim R.H., Huh T.L., and Park J.W. α-Phenyl-N-t-butylnitrone protects oxidative damage to HepG2 cells. J. Biochem. Mol. Biol. 34 (2001) 43-46
28. Moya M.A., Ramos S., Mateos R., Granado Serrano A.B., Izquierdo-Pulido M., Bravo L., and Goya L. Protection of human Hepg2 cells against oxidative stress by cocoa phenolic extract. J. Agric. Food Chem. 56 (2008) 7765-7772
29. Thabrew M.I., Hughes R.D., and Mcfarlane I.G. Screening of hepatoprotective plant components using a HepG2 cell cytotoxicity assay. J. Pharm. Pharmacol. 49 (1997) 1132-1135
30. Tripathi A.K., Verma R.K., Gupta A.K., Gupta M.M., and Khanuja S.P. Quantitative determination of phyllanthin and hypophyllanthin in Phyllanthus species by high-performance thin layer chromatography. Phytochem. Anal. 17 (2006) 394-397
31. Rao Y.K., Geethangili M., Fang S.H., and Tzeng Y.M. Antioxidant and cytotoxic activities of naturally occurring phenolic and related compounds: a comparative study. Food Chem. Toxicol. 45 (2008) 1770-1776
32. Harries H.M., Fletcher S.T., Duggan C.M., and Baker V.A. The use of genomics technology to investigate gene expression changes in cultured human liver cells. Toxicol. In Vitro 15 (2001) 399-405
33. Wu D., and Cederbaum A.I. Development and properties of HepG2 cells that constitutively express CYP2E1. Methods Mol. Biol. 447 (2008) 137-150
34. Lowry H.D., Rosenberg N.J., Farr A.L., and Randu R.J. Protein measurement with Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
35. Fang S.H., Rao Y.K., and Tzeng Y.M. Anti-oxidant and inflammatory mediator's growth inhibitory effects of compounds isolated from Phyllanthus urinaria. J. Ethnopharmacol. 116 (2008) 333-340
36. Sassa S., Sugita O., Galbraith R.A., and Kappas A. Drug metabolism by the human hepatoma cell, HepG2 27. Biochem. Biophys. Res. Commun. 143 (1987) 52-57
37. Duthie S.J., Melvin W.T., and Burke M.D. Bromobenzene detoxification in the human, liver-derived HepG2 cell line. Xenobiotica 24 (1994) 265-279
38. Bouma M.E., Rogier E., Verthier N., Labarre C., and Feldmann G. Further cellular investigation of the human hepatoblastoma-derived cell line HepG2: morphology and immunocytochemical studies of hepatic-secreted proteins. In Vitro Cell. Dev. Biol. 25 (1989) 267-275
39. Lima C.F., Valentao P.C.R., Andrade P.B., Seabra R.M., Fernandes-Ferreira M., and Pereira-Wilson C. Water and methanolic extracts of Salvia officinalis protect HepG2 cells from t-BHP induced oxidative damage. Chem. Biol. Interact. 167 (2007) 107-115
40. 4-induced hydroperoxide generation and cytotoxicity possibly through C1b receptor in rat hepatocytes. Biochem. Biophys. Res. Commun. 218 (1996) 865-871
41. Patlolla A.K., Barnes C., Hackett D., and Tchounwou P.B. Potassium dichromate induced cytotoxicity, genotoxicity and oxidative stress in human liver carcinoma (HepG2) cells. Int. J. Environ. Res. Public Health 6 (2009) 643-653
42. 4-induced toxicity in isolated hepatocytes: the importance of direct solvent injury. Hepatology 6 (1986) 36-45
43. Holden P.R., James N.H., Brooks A.N., Roberts R.A., Kimber I., and Pennie W.D. Identification of a possible association between carbon tetrachloride-induced hepatotoxicity and interleukin-8 expression. J. Biochem. Mol. Toxicol. 14 (2000) 283-290
44. Marinovich M., Lorenzo J.L., Flaminio L.M., Granata A., and Galli C.L. The HepG2 cell line as a possible alternative to isolated hepatocytes in cytotoxicity studies. ATLA 16 (1988) 16-22
45. Gutierrez-Ruiz M.C., Quiroz S.C., Souza V., Bucio L., Hernandez E., Olivares I.P., Llorente I., Vargas-Vorackova F., and Kershenobich D. Cytokines, growth factors, and oxidative stress in HepG2 cells treated with ethanol, acetaldehyde, and LPS. Toxicology 134 (1999) 197-207
46. Yasuda H., Izumi N., Shimada O., Kobayakawa T., and Nakanishi M. The protective effect of tinoridine against carbon tetrachloride hepatotoxicity. Toxicol. Appl. Pharmacol. 52 (1980) 407-413