Comparative analysis of circulating tumor DNA stability In K3EDTA, Streck, and CellSave blood collection tubes

Clinical Biochemistry

Volumn 49, Issue 18, 2016, Pages 1354-1360

  Kang, Qing ^a   Henry, N Lynn ^a   Paoletti, Costanza ^a   Jiang, Hui ^b   Vats, Pankaj ^c   Chinnaiyan, Arul M ^a,c,d   Hayes, Daniel F ^a   Merajver, Sofia D ^a,b   Rae, James M ^a   Tewari, Muneesh ^a,b  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

^c UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

^d HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

CellSave; Circulating tumor DNA; Droplet digital PCR; Streck BCT

Indexed keywords

CIRCULATING TUMOR DNA; EDETIC ACID; GENOMIC DNA; ICE; UNCLASSIFIED DRUG; DNA;

ADULT; ARTICLE; BLOOD COLLECTION TUBE; CLINICAL ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; HUMAN; METASTATIC BREAST CANCER; MOLECULAR STABILITY; MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; ROOM TEMPERATURE; STORAGE TEMPERATURE; WILD TYPE; BLOOD; CHEMISTRY; DEVICES; SPECIMEN HANDLING;

DNA, NEOPLASM; EDETIC ACID; HUMANS; SPECIMEN HANDLING;

EID: 84967239521     PISSN: 00099120     EISSN: 18732933     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2016.03.012     Document Type: Article

References (24)

1. [1] Bettegowda, C., Sausen, M., Leary, R.J., Kinde, I., Wang, Y., Agrawal, N., Detection of circulating tumor DNA in early- and late-stage human malignancies. Sci. Transl. Med., 6, 2014, 224ra24.
2. [2] Crowley, E., Di Nicolantonio, F., Loupakis, F., Bardelli, A., Liquid biopsy: monitoring cancer-genetics in the blood. Nat. Rev. Clin. Oncol. 10 (2013), 472–484.
3. [3] Siravegna, G., Mussolin, B., Buscarino, M., Corti, G., Cassingena, A., Crisafulli, G., Clonal evolution and resistance to egfr blockade in the blood of colorectal cancer patients. Nat. Med., 2015.
4. [4] Frenel, J.S., Carreira, S., Goodall, J., Roda Perez, D., Perez Lopez, R., Tunariu, N., Serial next generation sequencing of circulating cell free DNA evaluating tumour clone response to molecularly targeted drug administration. Clin. Cancer Res., 2015.
5. [5] Thress, K.S., Paweletz, C.P., Felip, E., Cho, B.C., Stetson, D., Dougherty, B., Acquired egfr c797s mutation mediates resistance to azd9291 in non-small cell lung cancer harboring egfr t790m. Nat. Med. 21 (2015), 560–562.
6. [6] Guttery, D.S., Page, K., Hills, A., Woodley, L., Marchese, S.D., Rghebi, B., Noninvasive detection of activating estrogen receptor 1 (esr1) mutations in estrogen receptor-positive metastatic breast cancer. Clin. Chem. 61 (2015), 974–982.
7. [7] Chang-Hao Tsao, S., Weiss, J., Hudson, C., Christophi, C., Cebon, J., Behren, A., Monitoring response to therapy in melanoma by quantifying circulating tumour DNA with droplet digital pcr for braf and nras mutations. Sci. Rep., 5, 2015, 11198.
8. [8] Olsson, E., Winter, C., George, A., Chen, Y., Howlin, J., Tang, M.H., Serial monitoring of circulating tumor DNA in patients with primary breast cancer for detection of occult metastatic disease. EMBO Mol. Med., 2015.
9. [9] Fernando, M.R., Chen, K., Norton, S., Krzyzanowski, G., Bourne, D., Hunsley, B., A new methodology to preserve the original proportion and integrity of cell-free fetal DNA in maternal plasma during sample processing and storage. Prenat. Diagn. 30 (2010), 418–424.
10. [10] Norton, S.E., Lechner, J.M., Williams, T., Fernando, M.R., A stabilizing reagent prevents cell-free DNA contamination by cellular DNA in plasma during blood sample storage and shipping as determined by digital pcr. Clin. Biochem. 46 (2013), 1561–1565.
11. [11] Wong, D., Moturi, S., Angkachatchai, V., Mueller, R., DeSantis, G., van den Boom, D., Optimizing blood collection, transport and storage conditions for cell free DNA increases access to prenatal testing. Clin. Biochem. 46 (2013), 1099–1104.
12. [12] Eliane, J.P., Repollet, M., Luker, K.E., Brown, M., Rae, J.M., Dontu, G., Monitoring serial changes in circulating human breast cancer cells in murine xenograft models. Cancer Res. 68 (2008), 5529–5532.
13. [13] Shaffer, D.R., Leversha, M.A., Danila, D.C., Lin, O., Gonzalez-Espinoza, R., Gu, B., Circulating tumor cell analysis in patients with progressive castration-resistant prostate cancer. Clin. Cancer Res. 13 (2007), 2023–2029.
14. [14] Roychowdhury, S., Iyer, M.K., Robinson, D.R., Lonigro, R.J., Wu, Y.M., Cao, X., Personalized oncology through integrative high-throughput sequencing: a pilot study. Sci. Transl. Med., 3, 2011, 111ra21.
15. [15] Rago, C., Huso, D.L., Diehl, F., Karim, B., Liu, G., Papadopoulos, N., Serial assessment of human tumor burdens in mice by the analysis of circulating DNA. Cancer Res. 67 (2007), 9364–9370.
16. [16] Barrett, A.N., Zimmermann, B.G., Wang, D., Holloway, A., Chitty, L.S., Implementing prenatal diagnosis based on cell-free fetal DNA: accurate identification of factors affecting fetal DNA yield. PLoS One, 6, 2011, e25202.
17. [17] Norton, S.E., Luna, K.K., Lechner, J.M., Qin, J., Fernando, M.R., A new blood collection device minimizes cellular DNA release during sample storage and shipping when compared to a standard device. J. Clin. Lab. Anal. 27 (2013), 305–311.
18. [18] Moore, H.M., Kelly, A.B., Jewell, S.D., McShane, L.M., Clark, D.P., Greenspan, R., Biospecimen reporting for improved study quality (brisq). J. Proteome Res. 10 (2011), 3429–3438.
19. [19] Denis, M.G., Knol, A.C., Theoleyre, S., Vallee, A., Dreno, B., Efficient detection of braf mutation in plasma of patients after long-term storage of blood in cell-free DNA blood collection tubes. Clin. Chem. 61 (2015), 886–888.
20. [20] Toro, P.V., Erlanger, B., Beaver, J.A., Cochran, R.L., VanDenBerg, D.A., Yakim, E., Comparison of cell stabilizing blood collection tubes for circulating plasma tumor DNA. Clin. Biochem. 48 (2015), 993–998.
21. [21] Barra, G.B., Santa Rita, T.H., Vasques, J.A., Chianca, C.F., Nery, L.F., Costa, S.S., Edta-mediated inhibition of dnases protects circulating cell-free DNA from ex vivo degradation in blood samples. Clin. Biochem. 48 (2015), 976–981.
22. [22] Paoletti, C.S.J., Hayes, D.F., Circulating tumor cells as a marker of prognosis. Princip. Pract. Oncol. 26 (2012), 1–8.
23. [23] Allard, W.J.H.D., Repollet, M.I., Rao, C., Herman, M.L., Matera, J., Miller, M.C., Terstappen, L.W., A cellular preservative improves the specificity and yield of circulating tumor cells in carcinoma patients. Proc. Am. Soc. Clin. Oncol., 22, 2003.
24. [24] Das, K., Fernando, M.R., Basiaga, S., Wigginton, S.M., Williams, T., Effects of a novel cell stabilizing reagent on DNA amplification by pcr as compared to traditional stabilizing reagents. Acta Histochem. 116 (2014), 55–60.