Real-time quantification of single RNA translation dynamics in living cells

Science

Volumn 352, Issue 6292, 2016, Pages 1425-1429

  Morisaki, Tatsuya ^a   Lyon, Kenneth ^a   DeLuca, Keith F ^a   DeLuca, Jennifer G ^a   English, Brian P ^b   Zhang, Zhengjian ^b   Lavis, Luke D ^b   Grimm, Jonathan B ^b   Viswanathan, Sarada ^b   Looger, Loren L ^b   Lionnet, Timothee ^b   Stasevich, Timothy J ^a  

^a Veterinary Dentistry and Oral Surgery   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; EPITOPE; FLUORESCENT DYE; MESSENGER RNA; NUCLEOTIDE; ANTIBODY; GREEN FLUORESCENT PROTEIN; HISTONE DEMETHYLASE; KDM5B PROTEIN, HUMAN; NUCLEAR PROTEIN; REPRESSOR PROTEIN;

AMINO ACID; ANTIBODY; CELLS AND CELL COMPONENTS; PROTEIN; QUANTITATIVE ANALYSIS; REACTION KINETICS; REAL TIME; RNA;

ANALYTIC METHOD; ARTICLE; CELL MOTILITY; CELL SIZE; GENE REGULATORY NETWORK; GENETIC TRANSCRIPTION AND TRANSLATION; KINETICS; NASCENT CHAIN TRACKING; POLYSOME; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RIBOSOME; RNA TRANSLATION; SENSITIVITY ANALYSIS; TRANSLATION INITIATION; BIOSYNTHESIS; CHEMISTRY; GENETICS; HUMAN; METABOLISM; MOLECULAR IMAGING; PHYSIOLOGY; PROCEDURES; SINGLE CELL ANALYSIS; TIME FACTOR;

ANTIBODIES; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; JUMONJI DOMAIN-CONTAINING HISTONE DEMETHYLASES; KINETICS; MOLECULAR IMAGING; NUCLEAR PROTEINS; POLYRIBOSOMES; PROTEIN BIOSYNTHESIS; REPRESSOR PROTEINS; RNA, MESSENGER; SINGLE-CELL ANALYSIS; TIME FACTORS;

EID: 84966441149     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aaf0899     Document Type: Article

References (26)

1. G. L. Hager, J. G. McNally, T. Misteli, Mol. Cell 35, 741-753 (2009).
2. X. Darzacq et al., Annu. Rev. Biophys. 38, 173-196 (2009).
3. J. A. Chao, Y. J. Yoon, R. H. Singer, Cold Spring Harb. Perspect. Biol. 4, a012310-a012310 (2012).
4. J. M. Halstead et al., Science 347, 1367-1671 (2015).
5. S. Viswanathan et al., Nat. Methods 12, 568-576 (2015).
6. E. Bertrand et al., Mol. Cell 2, 437-445 (1998).
7. J. B. Grimm et al., Nat. Methods 12, 244-250 (2015).
8. G. Blobel, D. Sabatini, Proc. Natl. Acad. Sci. U.S.A. 68, 390-394 (1971).
9. J. R. Warner, A. Rich, C. E. Hall, Science 138, 1399-1403 (1962).
10. A. K. Christensen, C. M. Bourne, Anat. Rec. 255, 116-129 (1999).
11. G. Viero et al., J. Cell Biol. 208, 581-596 (2015).
12. D. R. Larson, D. Zenklusen, B. Wu, J. A. Chao, R. H. Singer, Science 332, 475-478 (2011).
13. H. Kimura, P. R. Cook, J. Cell Biol. 153, 1341-1354 (2001).
14. F. Mueller, T. Morisaki, D. Mazza, J. G. McNally, Biophys. J. 102, 1656-1665 (2012).
15. D. R. Larson, R. H. Singer, D. Zenklusen, Trends Cell Biol. 19, 630-637 (2009).
16. J. Yu, J. Xiao, X. Ren, K. Lao, X. S. Xie, Science 311, 1600-1603 (2006).
17. N. T. Ingolia, S. Ghaemmaghami, J. R. S. Newman, J. S. Weissman, Science 324, 218-223 (2009).
18. N. T. Ingolia, L. F. Lareau, J. S. Weissman, Cell 147, 789-802 (2011).
19. J.-R. Yang, X. Chen, J. Zhang, PLOS Biol. 12, e1001910 (2014).
20. R. D. Palmiter, Cell 4, 189-197 (1975).
21. R. Jackson, N. Standart, RNA 21, 652-654 (2015).
22. G. S. Wilkie, K. S. Dickson, N. K. Gray, Trends Biochem. Sci. 28, 182-188 (2003).
23. N. Sonenberg, A. G. Hinnebusch, Cell 136, 731-745 (2009).
24. Y.-W. Shieh et al., Science 350, 678-680 (2015).
25. B. Wu, C. Eliscovich, Y. J. Yoon, R. H. Singer, Science 352, 1430-1435 (2016).
26. M. E. Tanenbaum, L. A. Gilbert, L. S. Qi, J. S. Weissman, R. D. Vale, Cell 159, 635-646 (2014).