Comparison of SYBR green i real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco

BMC Research Notes

Volumn 9, Issue 1, 2016, Pages

  Fellahi, Siham ^a,b   Harrak, Mehdi El ^c   Kuhn, Jens H ^d   Sebbar, Ghizlane ^c   Bouaiti, El Arbi ^e   Khataby, Khadija ^b   Fihri, Ouafae Fassi ^a   Houadfi, Mohammed El ^a   Ennaji, My Mustapha ^b  

^a Veterinary Institute Hassan II   (Morocco)

^b Faculty of Sciences and Technologies Mohammedia   (Morocco)

^c Society of Biological Products and Veterinary Pharmaceuticals   (Morocco)

^d NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^e MOHAMMED V UNIVERSITY   (Morocco)

Author keywords

Coronavirus; Gammacoronavirus; IBV; Infectious bronchitis virus; Real time RT PCR; RT PCR; SYBR green

Indexed keywords

NUCLEOCAPSID PROTEIN; NUCLEOCAPSID PROTEIN, CORONAVIRUS; ORGANIC COMPOUND; SYBR GREEN I; VIRUS RNA;

AGAR GEL ELECTROPHORESIS; ANIMAL; AVIAN INFECTIOUS BRONCHITIS VIRUS; BIRD DISEASES; CHICKEN; COMPARATIVE STUDY; CORONAVIRUS INFECTIONS; DISEASE OUTBREAKS; GENETICS; MOROCCO; PROCEDURES; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIROLOGY;

ANIMALS; BIRD DISEASES; CHICKENS; CORONAVIRUS INFECTIONS; DISEASE OUTBREAKS; ELECTROPHORESIS, AGAR GEL; INFECTIOUS BRONCHITIS VIRUS; MOROCCO; NUCLEOCAPSID PROTEINS; ORGANIC CHEMICALS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

EID: 84966351088     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/s13104-016-2037-z     Document Type: Article

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