Engineering Transcriptional Regulator Effector Specificity Using Computational Design and in Vitro Rapid Prototyping: Developing a Vanillin Sensor

ACS Synthetic Biology

Volumn 5, Issue 4, 2016, Pages 287-295

  De Los Santos, Emmanuel L C ^a,b   Meyerowitz, Joseph T ^a   Mayo, Stephen L ^a   Murray, Richard M ^a  

^a California Institute of Technology   (United States)

^b UNIVERSITY OF WARWICK   (United Kingdom)

Author keywords

cell free systems; computational protein design; in vitro biological circuit prototyping; protein engineering; synthetic biology; TX TL

Indexed keywords

TRANSCRIPTION FACTOR; VANILLIN; BACTERIAL PROTEIN; BENZALDEHYDE DERIVATIVE; QACR PROTEIN, STAPHYLOCOCCUS AUREUS; REPRESSOR PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BIOFUEL PRODUCTION; CELL MEMBRANE PERMEABILITY; COMPUTER MODEL; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; DNA BINDING; FEEDBACK SYSTEM; GENETIC ENGINEERING; HYDROGEN BOND; IN VITRO STUDY; IN VIVO STUDY; PRIORITY JOURNAL; PROCESS DESIGN; PROTEIN EXPRESSION; REGULATORY MECHANISM; REPRESSOR GENE; SENSOR; CELL FREE SYSTEM; CHEMISTRY; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; MUTAGENESIS; PLASMID; PROTEIN ENGINEERING; PROTEIN SYNTHESIS; STAPHYLOCOCCUS AUREUS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BENZALDEHYDES; CELL-FREE SYSTEM; ESCHERICHIA COLI; MUTAGENESIS; PLASMIDS; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; REPRESSOR PROTEINS; STAPHYLOCOCCUS AUREUS; TRANSCRIPTION, GENETIC;

EID: 84966298459     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.5b00090     Document Type: Article

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