Visualization of RelB expression and activation at the single-cell level during dendritic cell maturation in Relb-Venus knock-in mice

Journal of Biochemistry

Volumn 158, Issue 6, 2015, Pages 485-495

  Seki, Takao ^a   Yamamoto, Mami ^a   Taguchi, Yuu ^a   Miyauchi, Maki ^a   Akiyama, Nobuko ^a   Yamaguchi, Noritaka ^b   Gohda, Jin ^a   Akiyama, Taishin ^a   Inoue, Jun Ichiro ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b CHIBA UNIVERSITY   (Japan)

Author keywords

dendritic cell; NF B; RelB; single cell; venus

Indexed keywords

HYBRID PROTEIN; TRANSCRIPTION FACTOR RELB; BACTERIAL PROTEIN; CD86 ANTIGEN; PHOTOPROTEIN; RELB PROTEIN, MOUSE; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL MATURATION; COLON; CONTROLLED STUDY; CYTOPLASM; DENDRITIC CELL; EMBRYO; FEMALE; HISTOPATHOLOGY; IMMUNOBLOTTING; IMMUNOCOMPETENT CELL; LYMPHOID ORGAN; MALE; MOUSE; NONHUMAN; PROTEIN EXPRESSION; SPLEEN; THYMUS; ANIMAL; B LYMPHOCYTE; C57BL MOUSE; CELL DIFFERENTIATION; CYTOLOGY; GENE; GENE EXPRESSION REGULATION; GENE TARGETING; GENETICS; IMMUNOLOGY; METABOLISM; REPORTER GENE; SIGNAL TRANSDUCTION; SINGLE CELL ANALYSIS;

ANIMALS; ANTIGENS, CD86; B-LYMPHOCYTES; BACTERIAL PROTEINS; CELL DIFFERENTIATION; CELLS, CULTURED; DENDRITIC CELLS; FEMALE; GENE EXPRESSION REGULATION; GENE KNOCK-IN TECHNIQUES; GENES, MHC CLASS II; GENES, REPORTER; LUMINESCENT PROTEINS; MALE; MICE; MICE, INBRED C57BL; SIGNAL TRANSDUCTION; SINGLE-CELL ANALYSIS; SPLEEN; THYMUS GLAND; TRANSCRIPTION FACTOR RELB;

EID: 84966289948     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvv064     Document Type: Article

References (31)

1. Karin, M. (2006) Nuclear factor-kB in cancer development and progression. Nature 441, 431-436
2. Basak, S., Kim, H., Kearns, J. D., Tergaonkar, V., O'Dea, E., Werner, S. L., Benedict, C. A., Ware, C. F., Ghosh, G., Verma, I. M., and Hoffmann, A. (2007) A fourth IkB protein within the NF-kB signaling module. Cell 128, 369-381
3. Basak, S., Shih, V. F., and Hoffmann, A. (2008) Generation and activation of multiple dimeric transcription factors within the NF-kB signaling system. Mol. Cell. Biol. 28, 3139-3150
4. Basak, S., Behar, M., and Hoffmann, A. (2012) Lessons from mathematically modeling the NF-kB pathway. Immunol. Rev. 246, 221-238
5. Hayden, M. S., and Ghosh, S. (2008) Shared principles in NF-kB signaling. Cell 132, 344-362
6. Oeckinghaus, A., Hayden, M. S., and Ghosh, S. (2011) Crosstalk in NF-kB signaling pathways. Nat. Immunol. 12, 695-708
7. Razani, B., Reichardt, A. D., and Cheng, G. (2011) Noncanonical NF-kB signaling activation and regulation: principles and perspectives. Immunol. Rev. 244, 44-54
8. Sun, S. C. (2011) Non-canonical NF-kB signaling pathway. Cell Res. 21, 71-85
9. Sun, S. C. (2012) The noncanonical NF-kB pathway. Immunol. Rev. 246, 125-140
10. Ghosh, S. And Hayden, M. S. (2008) New regulators of NF-kB in inflammation. Nat. Rev. Immunol. 8, 837-848
11. Vallabhapurapu, S. And Karin, M. (2009) Regulation and function of NF-kB transcription factors in the immune system. Annu. Rev. Immunol. 27, 693-733
12. Wu, L., D'Amico, A., Winkel, K. D., Suter, M., Lo, D., and Shortman, K. (1998) RelB is essential for the development of myeloid-related CD8a-dendritic cells but not of lymphoid-related CD8a+ dendritic cells. Immunity 9, 839-847
13. De Lorenzi, R., Gareus, R., Fengler, S., and Pasparakis, M. (2009) GFP-p65 knock-in mice as a tool to study NFkB dynamics in vivo. Genesis 47, 323-329
14. Sakai, K. And Miyazaki, J. (1997) A transgenic mouse line that retains Cre recombinase activity in mature oocytes irrespective of the cre transgene transmission. Biochem. Biophys. Res. Commun. 237, 318-324
15. Ley, T. J., Hug, B., Fiering, S., Epner, E., Bender, M. A., and Groudine, M. (1998) Reduced b-globin gene expression in adult mice containing deletions of locus control region 5' HS-2 or 5' HS-3. Ann. NY Acad. Sci. 850, 45-53
16. Carrasco, D., Ryseck, R. P., and Bravo, R. (1993) Expression of relB transcripts during lymphoid organ development: specific expression in dendritic antigenpresenting cells. Development 118, 1221-1231
17. Shih, V. F., Davis-Turak, J., Macal, M., Huang, J. Q., Ponomarenko, J., Kearns, J. D., Yu, T., Fagerlund, R., Asagiri, M., Zuniga, E. I., and Hoffmann, A. (2012) Control of RelB during dendritic cell activation integrates canonical and noncanonical NF-kB pathways. Nat. Immunol. 13, 1162-1170
18. Zanetti, M., Castiglioni, P., Schoenberger, S., and Gerloni, M. (2003) The role of relB in regulating the adaptive immune response. Ann. NY Acad. Sci. 987, 249-257
19. Wu, L. And Shortman, K. (2005) Heterogeneity of thymic dendritic cells. Semin. Immunol. 17, 304-312
20. Wilson, N. S. And Villadangos, J. A. (2005) Regulation of antigen presentation and cross-presentation in the dendritic cell network: facts, hypothesis, and immunological implications. Adv. Immunol. 86, 241-305
21. Denning, T. L., Norris, B. A., Medina-Contreras, O., Manicassamy, S., Geem, D., Madan, R., Karp, C. L., Pulendran, B. (2011) Functional specializations of intestinal dendritic cell and macrophage subsets that control Th17 and regulatory T cell responses are dependent on the T cell/APC ratio, source of mouse strain, and regional localization. J. Immunol. 187, 733-747
22. del Rio, M. L., Bernhardt, G., Rodriguez-Barbosa, J. I., and Fö rster, R. (2010) Development and functional specialization of CD103+ dendritic cells. Immunol. Rev. 234, 268-281
23. Moriwaki, K., Balaji, S., McQuade, T., Malhotra, N., Kang, J., and Chan, F. K. (2014) The necroptosis adaptor RIPK3 promotes injury-induced cytokine expression and tissue repair. Immunity 41, 567-578
24. Covert, M. W., Leung, T. H., Gaston, J. E., and Baltimore, D. (2005) Achieving stability of lipopolysaccharide-induced NF-kappaB activation. Science 309, 1854-1857
25. Hoffmann, A., Levchenko, A., Scott, M. L., and Baltimore, D. (2002) The IkB-NF-kB signaling module: Temporal control and selective gene activation. Science 298, 1241-1245
26. Nelson, D. E., Ihekwaba, A. E., Elliott, M., Johnson, J. R., Gibney, C. A., Foreman, B. E., Nelson, G., See, V., Horton, C. A., Spiller, D. G., Edwards, S. W., McDowell, H. P., Unitt, J. F., Sullivan, E., Grimley, R., Benson, N., Broomhead, D., Kell, D. B., and White, M. R. (2004) Oscillations in NF-kB signaling control the dynamics of gene expression. Science 306, 704-708
27. Lee, T. K., Denny, E. M., Sanghvi, J. C., Gaston, J. E., Maynard, N. D., Hughey, J. J., and Covert, M. W. (2009) A noisy paracrine signal determines the cellular NF-kB response to lipopolysaccharide. Sci. Signal 2, ra65
28. Ashall, L., Horton, C. A., Nelson, D. E., Paszek, P., Harper, C. V., Sillitoe, K., Ryan, S., Spiller, D. G., Unitt, J. F., Broomhead, D. S., Kell, D. B., Rand, D. A., S-ee, V., and White, M. R. (2009) Pulsatile stimulation determines timing and specificity of NF-kB-dependent transcription. Science 324, 242-246
29. Tay, S., Hughey, J. J., Lee, T. K., Lipniacki, T., Quake, S. R., and Covert, M. W. (2010) Single-cell NF-kB dynamics reveal digital activation and analogue information processing. Nature 466, 267-271
30. Sung, M. H., Salvatore, L., De Lorenzi, R., Indrawan, A., Pasparakis, M., Hager, G. L., Bianchi, M. E., Agresti, A. (2009) Sustained oscillations of NF-kB produce distinct genome scanning and gene expression profiles. PLoS One 4, e7163
31. Kellogg, R. A., and Tay, S. (2015) Noise facilitates transcriptional control under dynamic inputs. Cell 160, 381-392