Optimized dissociation protocol for isolating human glioma stem cells from tumorspheres via fluorescence-activated cell sorting

Cancer Letters

Volumn 377, Issue 1, 2016, Pages 105-115

  Lv, Donglai ^a,b   Ma, Qing hua ^a,b   Duan, Jiang jie ^a,b   Wu, Hai bo ^a,b   Zhao, Xi long ^a,b   Yu, Shi cang ^a,b   Bian, Xiu wu ^a,b  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

^b BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

Author keywords

CD133; Cell dissociation; Flow cytometry; Glioma; Stem cell

Indexed keywords

ACCUTASE; CD133 ANTIGEN; LIBERASE; NON ENZYMATIC CELL DISSOCIATION SOLUTION; REAGENT; TRYPSIN; UNCLASSIFIED DRUG; COLLAGENASE; PEPTIDE HYDROLASE; PROM1 PROTEIN, HUMAN; THERMOLYSIN; TUMOR MARKER;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIGENICITY; ARTICLE; CELL DIFFERENTIATION; CELL ISOLATION; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; CULTURE OPTIMIZATION; DISSOCIATION; FEMALE; FLUORESCENCE ACTIVATED CELL SORTING; GLIOMA STEM CELL; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; STEM CELL SELF-RENEWAL; TUMOR XENOGRAFT; TUMORSPHERE; ANIMAL; CANCER STEM CELL; CELL SELF-RENEWAL; CELL SEPARATION; CELL SURVIVAL; COMPARATIVE STUDY; FLOW CYTOMETRY; GLIOMA; METABOLISM; MULTICELLULAR SPHEROID; MULTIPOTENT STEM CELL; NUDE MOUSE; PATHOLOGY; PHENOTYPE; PROCEDURES; TIME FACTOR; TUMOR CELL LINE; TUMOR VOLUME;

AC133 ANTIGEN; ANIMALS; BIOMARKERS, TUMOR; CELL LINE, TUMOR; CELL SELF RENEWAL; CELL SEPARATION; CELL SURVIVAL; COLLAGENASES; FEMALE; FLOW CYTOMETRY; GLIOMA; HUMANS; MICE, NUDE; MULTIPOTENT STEM CELLS; NEOPLASTIC STEM CELLS; PEPTIDE HYDROLASES; PHENOTYPE; SPHEROIDS, CELLULAR; THERMOLYSIN; TIME FACTORS; TRYPSIN; TUMOR BURDEN;

EID: 84964863842     PISSN: 03043835     EISSN: 18727980     Source Type: Journal    
DOI: 10.1016/j.canlet.2016.04.022     Document Type: Article

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