Rho kinase inhibitor Y-27632 and Accutase dramatically increase mouse embryonic stem cell derivation

In Vitro Cellular and Developmental Biology - Animal

Volumn 48, Issue 1, 2012, Pages 30-36

  Zhang, Peng ^a,b   Wu, Xinglong ^a   Hu, Chunchao ^a   Wang, Pengbo ^a   Li, Xiangyun ^a  

^a AGRICULTURAL UNIVERSITY OF HEBEI   (China)

^b SUN YAT SEN UNIVERSITY   (China)

Author keywords

Accutase; Derivation; Embryonic stem cells; Mouse; Y 27632

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; ACCUTASE; AMIDE; COLLAGENASE; PEPTIDE HYDROLASE; PYRIDINE DERIVATIVE; RHO KINASE;

ANIMAL; ARTICLE; BLASTOCYST; CELL ADHESION; CHEMISTRY; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; EMBRYONIC STEM CELL; METABOLISM; MOUSE;

AMIDES; ANIMALS; BLASTOCYST; CELL ADHESION; CELL CULTURE TECHNIQUES; COLLAGENASES; CULTURE MEDIA; EMBRYONIC STEM CELLS; MICE; PEPTIDE HYDROLASES; PYRIDINES; RHO-ASSOCIATED KINASES;

EID: 84857234111     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11626-011-9471-y     Document Type: Article

References (21)

1. Bajpai R.; Lesperance J.; Kim M.; Terskikh A. V. Efficient propagation of single cells Accutase-dissociated human embryonic stem cells. Mol. Reprod. Dev. 75(5): 818-827; 2008. (Pubitemid 351438945)
2. Batlle-Morera L.; Smith A.; Nichols J. Parameters influencing derivation of embryonic stem cells from murine embryos. Genesis 46(12): 758-767; 2008.
3. Brook F. A.; Gardner R. L. The origin and efficient derivation of embryonic stem cells in the mouse. Proc. Natl. Acad. Sci. U S A 94(11): 5709-5712; 1997. (Pubitemid 27241834)
4. Bryja V.; Bonilla S.; Cajánek L.; Parish C. L.; Schwartz C. M.; Luo Y.; Rao M. S.; Arenas E. An efficient method for the derivation of mouse embryonic stem cells. Stem Cells 24(4): 844-849; 2006. (Pubitemid 44464717)
5. Buehr M.; Meek S.; Blair K.; Yang J.; Ure J.; Silva J.; McLay R.; Hall J.; Ying Q. L.; Smith A. Capture of authentic embryonic stem cells from rat blastocysts. Cell 135(7): 1287-1298; 2008.
6. Chen G.; Hou Z.; Gulbranson D. R.; Thomson J. A. Actin-myosin contractility is responsible for the reduced viability of dissociated human embryonic stem cells. Cell Stem Cell 7(2): 240-248; 2010.
7. Cheng J.; Dutra A.; Takesono A.; Garrett-Beal L.; Schwartzberg P. L. Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media. Genesis 39: 100-104; 2004. (Pubitemid 38924657)
8. Claassen D. A.; Desler M. M.; Rizzino A. ROCK inhibition enhances the recovery and growth of cryopreserved human embryonic stem cells and human induced pluripotent stem cells. Mol. Reprod. Dev. 76(8): 722-732; 2009.
9. Doungpunta J.; Santhi A.; Sathanawongs A.; Jarujinda Y.; Oranratnachai A. Fivefold increase in derivation rates of mouse embryonic stem cellsafter supplementation of the media with multiple factors. Theriogenology 72(2): 232-242; 2009.
10. Evans M. J.; Kaufman M. H. Establishment in culture of pluripotential cells from mouse embryos. Nature 292(5819): 154-156; 1981. (Pubitemid 11050741)
11. Li X.; Krawetz R.; Liu S.; Meng G.; Rancourt D. E. ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells. Hum. Reprod. 24(3): 580-589; 2008.
12. Martin G. R. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc. Natl. Acad. Sci. U S A 78(12): 7634-7638; 1981. (Pubitemid 12186197)
13. McWhir J.; Schnieke A. E.; Ansell R.; Wallace H.; Colman A.; Scott A. R.; Kind A. J. Selective ablation of differentiated cells permits isolation of embryonic stem cell lines from murine embryos with a non-permissive genetic background. Nat. Genet. 14: 223-226; 1996. (Pubitemid 26338810)
14. Ohgushi M.; Matsumura M.; Eiraku M.; Murakami K.; Aramaki T.; Nishiyama A.; Muguruma K.; Nakano T.; Suga H.; Ueno M.; Ishizaki T.; Suemori H.; Narumiya S.; Niwa H.; Sasai Y. Molecular pathway and cell state responsible for dissociation-induced apoptosis in human pluripotent stem cells. Cell Stem Cell 7(2): 225-239; 2010.
15. Robertson E. J. Teratocarcinomas and Embryonic Stem Cells: A pratical approach. IRL Press, Oxford; 1987.
16. Schoonjans L.; Kreemers V.; Danloy S.; Moreadith R. W.; Laroche Y.; Collen D. Improved generation of germline competent embryonic stem cell lines from inbred mouse strains. Stem Cells 21(1): 90-97; 2003. (Pubitemid 36120369)
17. Tesar P. J. Derivation of germ-line-competent embryonic stemcell lines from preblastocyst mouse embryos. Proc. Natl. Acad. Sci. U S A 102(23): 8239-8244; 2005. (Pubitemid 40800073)
18. Wachs F. P.; Couillard-Despres S.; Engelhardt M.; Wilhelm D.; Ploetz S.; Vroemen M.; Kaesbauer J.; Uyanik G.; Klucken J.; Karl C.; Tebbing J.; Svendsen C.; Weidner N.; Kuhn H. G.; Winkler J.; Aigner L. High efficacy of clonal growth and expansion of adult neural stem cells. Lab. Investig. 83(7): 949-962; 2003. (Pubitemid 36899062)
19. Walker A.; Su H.; Conti M. A.; Harb N.; Adelstein R. S.; Sato N. Non-muscle myosin II regulates survival threshold of pluripotent stem cells. Nat. Commun. 1: 71; 2010.
20. Watanabe K.; Ueno M.; Kamiya D.; Nishiyama A.; Matsumura M.; Wataya T.; Takahashi J. B.; Nishikawa S.; Nishikawa S.; Muguruma K.; Sasai Y. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 25(6): 681-686; 2007. (Pubitemid 46897207)
21. Zheng K.; Liu T. Q.; Dai M. S.; Ge D.; Li X. Q.; Ma X. H.; Cui Z. F. Comparison of different culture modes for long-term expansion of neural stem cells. Cytotechnology 52(3): 209-218; 2006. (Pubitemid 46686119)