Real-Time TaqMan PCR Assay for the Detection of Heat-Labile and Heat-Stable Enterotoxin Genes in a Geographically Diverse Collection of Enterotoxigenic Escherichia coli Strains and Stool Specimens

Foodborne Pathogens and Disease

Volumn 13, Issue 4, 2016, Pages 212-220

  Pattabiraman, Vaishnavi ^a   Parsons, Michele B ^a   Bopp, Cheryl A ^a  

^a NATIONAL CENTER FOR EMERGING AND ZOONOTIC INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI ENTEROTOXIN; BACTERIAL DNA; ENTEROTOXIN; ESCHERICHIA COLI PROTEIN;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; ENTEROTOXIGENIC ESCHERICHIA COLI; ESCHERICHIA COLI INFECTION; FECES ANALYSIS; GOLD STANDARD; HUMAN; LIMIT OF DETECTION; NONHUMAN; POLYMERASE CHAIN REACTION SYSTEM; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; THERMOSTABILITY; VALIDATION PROCESS; CHEMISTRY; CLASSIFICATION; DYSENTERY; EPIDEMIC; EPIDEMIOLOGICAL MONITORING; ESCHERICHIA COLI INFECTIONS; FECES; GENETICS; HEAT; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOLECULAR TYPING; MULTIPLEX POLYMERASE CHAIN REACTION; PROTEIN STABILITY; PUBLIC HEALTH SERVICE; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TIME FACTOR; UNITED STATES; VALIDATION STUDY;

CENTERS FOR DISEASE CONTROL AND PREVENTION (U.S.); DISEASE OUTBREAKS; DNA, BACTERIAL; DYSENTERY; ENTEROTOXIGENIC ESCHERICHIA COLI; ENTEROTOXINS; EPIDEMIOLOGICAL MONITORING; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; FECES; HOT TEMPERATURE; HUMANS; LIMIT OF DETECTION; MOLECULAR TYPING; MULTIPLEX POLYMERASE CHAIN REACTION; PROTEIN STABILITY; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TIME FACTORS; UNITED STATES;

EID: 84964584467     PISSN: 15353141     EISSN: 15567125     Source Type: Journal    
DOI: 10.1089/fpd.2015.2064     Document Type: Article

References (30)

1. Atkinson R, Maguire H, Gerner-Smidt P. A challenge and an opportunity to improve patient management and public health surveillance for food-borne infections through cultureindependent diagnostics. J Clin Microbiol 2013;51:2479-82.
2. Banoo S, Bell D, Bossuyt P, et al. Evaluation of diagnostic tests for infectious diseases: General principles. Nature Rev Microbiol 2006;4:S21-31.
3. Black RE. Epidemiology of travelers' diarrhea and relative importance of various pathogens. Rev Infect Dis 1990;12:S73-79.
4. Buss SN, Leber A, Chapin K, et al. Multicenter Evaluation of the BioFire FilmArrayGastrointestinal Panel for EtiologicDiagnosis of Infectious Gastroenteritis. J Clin Microbiol 2015;53:915-925.
5. Di Cristanziano V, Timmen-Wego M, Lubke N, et al. Application of Luminex Gastrointestinal Pathogen Panel to human stool samples fromCote d'Ivoire. J InfectDevCtries 2015;9:884-889.
6. Dubreuil JD. The whole Shebang: The gastrointestinal tract, Escherichia coli enterotoxins and secretion. Curr Issues Mol Biol 2012;14:71-82.
7. Evans DJ, Jr, Evans DG, DuPont HL, Orskov F, Orskov I. Patterns of loss of enterotoxigenicity by Escherichia coli isolated from adults with diarrhea: Suggestive evidence for an interrelationship with serotype. Infect Immun 1977;17: 105-111.
8. Gorbach SL, Kean BH, Evans DG, Evans DJ, Jr, Bessudo D. Travelers' diarrhea and toxigenic Escherichia coli. NeEngl J Med 1975;292:933-936.
9. Grant MA, Hu J, Jinneman KC. Multiplex real-time PCR detection of heat-labile and heat-stable toxin genes in enterotoxigenic Escherichia coli. J Food Prot 2006;69:412-416.
10. Guerra JA, Romero-Herazo YC, Arzuza O, Gomez-Duarte OG. Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli clinical isolates from northern Colombia, South America. BioMed Res Int 2014;2014:236260.
11. Hill WE, Payne WL, Zon G, Moseley SL. Synthetic oligodeoxyribonucleotide probes for detecting heat-stable enterotoxinproducing Escherichia coli by DNA colony hybridization. Appl Environ Microbiol 1985;50:1187-1191.
12. Jones TF, Gerner-Smidt P. Nonculture diagnostic tests for enteric diseases. Emerging Infectious Diseases 2012;18:513-4.
13. Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol 2004;2:123-140.
14. Lindsay BR, Chakraborty S, Harro C, et al. Quantitative PCR and culture evaluation for enterotoxigenic Escherichia coli (ETEC) associated diarrhea in volunteers. FEMS Microbiol Lett 2014;352:25-31.
15. Ling D, Salvaterra PM. Robust RT-qPCR data normalization: Validation and selection of internal reference genes during post-experimental data analysis. PLoS One 2011;6:e17762.
16. Lortie LA, Dubreuil JD, Harel J. Characterization of Escherichia coli strains producing heat-stable enterotoxin b (STb) isolated from humans with diarrhea. J ClinMicrobiol 1991;29:656-659.
17. Murray BE, Mathewson JJ, DuPont HL, Hill WE. Utility of oligodeoxyribonucleotide probes for detecting enterotoxigenic Escherichia coli. J Infect Dis 1987;155:809-811.
18. Okamoto K, Fujii Y, Akashi N, et al. Identification and characterization of heat-stable enterotoxin II-producing Escherichia coli from patients with diarrhea. Microbiol Immunol 1993;37:411-4.
19. Okoh AI, Osode AN. Enterotoxigenic Escherichia coli (ETEC): A recurring decimal in infants' and travelers' diarrhea. Rev Environ Health 2008;23:135-148.
20. Pandey P, Bodhidatta L, Lewis M, et al. Travelers' diarrhea in Nepal: An update on the pathogens and antibiotic resistance. J Travel Med 2011;18:102-108.
21. Paschke C, Apelt N, Fleischmann E, et al. Controlled study on enteropathogens in travellers returning from the tropics with and without diarrhoea. Clin Microbiol Infect 2011;17:1194-1200.
22. Patel A, Navidad J, Bhattacharyya S. Site-specific clinical evaluation of the Luminex xTAG Gastrointestinal Pathogen Panel for Detection of Infectious Gastroenteritis in Fecal Specimens. J Clin Microbiol 2014;52:3068-3071.
23. Qadri F, Svennerholm AM, Faruque AS, Sack RB. Enterotoxigenic Escherichia coli in developing countries: Epidemiology, microbiology, clinical features, treatment, and prevention. Clin Microbiol Rev 2005;18:465-483.
24. Reischl U, Youssef MT, Wolf H, Hyytia-Trees E, Strockbine NA. Real-time fluorescence PCR assays for detection and characterization of heat-labile I and heat-stable I enterotoxin genes from enterotoxigenic Escherichia coli. J Clin Microbiol 2004;42:4092-4100.
25. Schultsz C, Pool GJ, van Ketel R, de Wever B, Speelman P, Dankert J. Detection of enterotoxigenic Escherichia coli in stool samples by using nonradioactively labeled oligonucleotide DNA probes and PCR. J Clin Microbiol 1994;32:2393-2397.
26. Sjoling A, Wiklund G, Savarino SJ, Cohen DI, Svennerholm AM. Comparative analyses of phenotypic and genotypic methods for detection of enterotoxigenic Escherichia coli toxins and colonization factors. J Clin Microbiol 2007;45:3295-3301.
27. von Mentzer A, Connor TR, Wieler LH, et al. Identification of enterotoxigenic Escherichia coli (ETEC) clades with longterm global distribution. Nat Genet 2014;46:1321-6.
28. [WHO] World Health Organization. Future directions for research on enterotoxigenic Escherichia coli vaccines for developing countries. Releve epidemiologique hebdomadaire. Wkly Epidemiol Rec 2006;81:97-104. (In English, French.)
29. Yoh M, Narita I, Honda T, Miwatani T, Nishibuchi M. Comparison of preservation methods for enterotoxigenic Escherichia coli producing heat-labile enterotoxin. J Clin Microbiol 1991;29:2326-2328.
30. Youmans BP, Ajami NJ, Jiang ZD, Petrosino JF, Dupont HL, Highlander SK. Development and accuracy of quantitative real-time polymerase chain reaction assays for detection and quantification of enterotoxigenic Escherichia coli (ETEC) heat labile and heat stable toxin genes in travelers' diarrhea samples. Am J Trop Med Hyg 2014;90:124-132.