Real-time monitoring of basal H2O2 levels with peroxiredoxin-based probes

Nature Chemical Biology

Volumn 12, Issue 6, 2016, Pages 437-443

  Morgan, Bruce ^a,b   Van Laer, Koen ^a   Owusu, Theresa N E ^a   Ezeriņa, Daria ^a   Pastor Flores, Daniel ^a   Amponsah, Prince Saforo ^a   Tursch, Anja ^a   Dick, Tobias P ^a  

^a GERMAN CANCER RESEARCH CENTER   (Germany)

^b University of Koblenz Landau   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROGEN PEROXIDE; OXYGEN; PEROXIREDOXIN; MOLECULAR PROBE;

ARTICLE; CELL GROWTH; CELL MIGRATION; CONTROLLED STUDY; CYTOSOL; GROWTH RATE; HOMEOSTASIS; MEASUREMENT; MITOCHONDRION; MOLECULAR PROBE; NONHUMAN; OXYGEN TENSION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SENSOR; CYTOLOGY; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PARTIAL PRESSURE; TIME FACTOR;

CYTOSOL; HOMEOSTASIS; HYDROGEN PEROXIDE; MITOCHONDRIA; MOLECULAR PROBES; OXYGEN; PARTIAL PRESSURE; PEROXIREDOXINS; SACCHAROMYCES CEREVISIAE; TIME FACTORS;

EID: 84964389843     PISSN: 15524450     EISSN: 15524469     Source Type: Journal    
DOI: 10.1038/nchembio.2067     Document Type: Article

References (31)

1. 2 generation: redox signaling and oxidative stress. J. Biol. Chem. 289, 8735-8741 (2014).
2. 2, a necessary evil for cell signaling. Science 312, 1882-1883 (2006).
3. Finkel, T. Signal transduction by reactive oxygen species. J. Cell Biol. 194, 7-15 (2011).
4. Fujikawa, Y., Morgan, B. & Dick, T.P. in Oxidative Stress and Redox Regulation (eds. Jakob, U. & Reichmann, D.) 119-156 (Springer, 2013).
5. Ludescher, C. et al. Detection of activity of P-glycoprotein in human tumour samples using rhodamine 123. Br. J. Haematol. 82, 161-168 (1992).
6. Huai-Yun, H. et al. Expression of multidrug resistance-associated protein (MRP) in brain microvessel endothelial cells. Biochem. Biophys. Res. Commun. 243, 816-820 (1998).
7. Belousov, V.V. et al. Genetically encoded fluorescent indicator for intracellular hydrogen peroxide. Nat. Methods 3, 281-286 (2006).
8. 2-scavenging peroxidases. J. Biol. Chem. 284, 31532-31540 (2009).
9. Schwarzländer, M., Dick, T.P., Meyer, A.J. & Morgan, B. Dissecting redox biology using fluorescent protein sensors. Antioxid. Redox Signal. doi:10.1089/ars.2015.6266 (2015).
10. Bilan, D. & Belousov, V. HyPer family probes: state of the art. Antioxid. Redox Signal. doi:10.1089/ars.2015.6586 (2016).
11. Ferrer-Sueta, G. et al. Factors affecting protein thiol reactivity and specificity in peroxide reduction. Chem. Res. Toxicol. 24, 434-450 (2011).
12. 2 receptor and redox-transducer in gene activation. Cell 111, 471-481 (2002).
13. 2 signaling. Nat. Chem. Biol. 11, 64-70 (2015).
14. Fomenko, D.E. et al. Thiol peroxidases mediate specific genome-wide regulation of gene expression in response to hydrogen peroxide. Proc. Natl. Acad. Sci. USA 108, 2729-2734 (2011).
15. 2: a quantitative comparison between continuous low-dose and one-time high-dose treatments. Free Radic. Biol. Med. 60, 325-335 (2013).
16. Huang, B.K. & Sikes, H.D. Quantifying intracellular hydrogen peroxide perturbations in terms of concentration. Redox Biol. 2C, 955-962 (2014).
17. 2 is not reflected in its reaction with other oxidants and thiol reagents. J. Biol. Chem. 282, 11885-11892 (2007).
18. Schröder, E. et al. Crystal structure of decameric 2-Cys peroxiredoxin from human erythrocytes at 1.7 Å resolution. Structure 8, 605-615 (2000).
19. Hall, A., Karplus, P.A. & Poole, L.B. Typical 2-Cys peroxiredoxins-structures, mechanisms and functions. FEBS J. 276, 2469-2477 (2009).
20. Ghaemmaghami, S. et al. Global analysis of protein expression in yeast. Nature 425, 737-741 (2003).
21. Morgan, B. et al. Multiple glutathione disulfide removal pathways mediate cytosolic redox homeostasis. Nat. Chem. Biol. 9, 119-125 (2013).
22. Gutscher, M. et al. Real-time imaging of the intracellular glutathione redox potential. Nat. Methods 5, 553-559 (2008).
23. Bode, M. et al. Inaccurately assembled cytochrome C oxidase can lead to oxidative stress-induced growth arrest. Antioxid. Redox Signal. 18, 1597-1612 (2013).
24. Merz, S. & Westermann, B. Genome-wide deletion mutant analysis reveals genes required for respiratory growth, mitochondrial genome maintenance and mitochondrial protein synthesis in Saccharomyces cerevisiae. Genome Biol. 10, R95 (2009).
25. Gomes, F., Tahara, E.B., Busso, C., Kowaltowski, A.J. & Barros, M.H. Nde1 deletion improves mitochondrial DNA maintenance in Saccharomyces cerevisiae coenzyme Q mutants. Biochem. J. 449, 595-603 (2013).
26. Davidson, J.F. & Schiestl, R.H. Mitochondrial respiratory electron carriers are involved in oxidative stress during heat stress in Saccharomyces cerevisiae. Mol. Cell. Biol. 21, 8483-8489 (2001).
27. Ezeriņa, D., Morgan, B. & Dick, T.P. Imaging dynamic redox processes with genetically encoded probes. J. Mol. Cell. Cardiol. 73, 43-49 (2014).
28. Stone, J.R. & Yang, S. Hydrogen peroxide: a signaling messenger. Antioxid. Redox Signal. 8, 243-270 (2006).
29. 2 with roGFP2-based redox probes. Free Radic. Biol. Med. 51, 1943-1951 (2011).
30. Janke, C. et al. A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast 21, 947-962 (2004).
31. Meyer, A.J. & Dick, T.P. Fluorescent protein-based redox probes. Antioxid. Redox Signal. 13, 621-650 (2010).