Optimization of a preparative RP-HPLC method for isolation and purification of picrosides in Picrorhiza kurroa

Journal of Plant Biochemistry and Biotechnology

Volumn 25, Issue 2, 2016, Pages 208-214

  Kumar, Varun ^a   Sood, Hemant ^a   Chauhan, Rajinder Singh ^a  

^a JAYPEE UNIVERSITY OF INFORMATION TECHNOLOGY   (India)

Author keywords

Isolation; Picroside I; Picroside II and prep HPLC; Purification

Indexed keywords

EID: 84962678709     PISSN: 09717811     EISSN: 09741275     Source Type: Journal    
DOI: 10.1007/s13562-015-0327-5     Document Type: Article

References (25)

1. Ansari RA, Tripathi SC, Patnaik GK, Dhawan BN (1991) Antihepatotoxic properties of picroliv: an active fraction from rhizomes of Picrorhiza kurrooa. J Ethnopharmacol 34:61–68. doi:10.1016/0378-8741(91)90189-k
2. Baruah CC, Gupta PP, Nath A, Patnaik LG, Dhawan BN (1998) Anti-allergic and anti-anaphylactic activity of picroliv–a standardised iridoid glycoside fraction of picrorhiza kurroa. Pharmacol Res: Off J Italian Pharmacol Soc 38:487–492. doi:10.1006/phrs.1998.0397
3. Bhandari P, Kumar N, Singh B, Gupta A, Kaul V, Ahuja P (2009) Stability-indicating LC–PDA method for determination of picrosides in hepatoprotective Indian herbal preparations of Picrorhiza kurroa. Chromatographia 69:221–227. doi:10.1365/s10337-008-0889-7
4. Chander R, Kapoor NK, Dhawan BN (1992) Picroliv, picroside-I and kutkoside from Picrorhiza kurrooa are scavengers of superoxide anions. Biochem Pharmacol 44:180–183. doi:10.1016/0006-2952(92)90054-m
5. Chen Z, Wu J, Shen W, Liu P, Cao Y, Lu Y (2011) Counter-current chromatographic method for preparative scale isolation of picrosides from traditional medicine picrorhiza scrophulariiflora. J Sep Sci 34:1910–1916. doi:10.1002/jssc.201100025
6. Gangola M, Parkash J, Ahuja P, Dutt S (2013) Components of antioxidant system of Picrorhiza kurrooa exhibit different spatio-temporal behavior Mol Biol Rep:1-11 doi:10.1007/s11033-013-2772-3
7. Gao H, Zhou YW (2005a) Anti-lipid peroxidation and protection of liver mitochondria against injuries by picroside ii World J Gastroenterol: WJG 11:3671–3674
8. Gao H, Zhou YW (2005b) Inhibitory effect of picroside ii on hepatocyte apoptosis acta. Pharmacol Sin 26:729–736. doi:10.1111/j.1745-7254.2005.00729.x
9. Guo Y, Xu X, Li Q, Li Z, Du F (2010) Anti-inflammation effects of picroside 2 in cerebral ischemic injury Rats. Behav Brain Funct 6:43. doi:10.1186/1744-9081-6-43
10. Guo YL, Shen W, Du F (2011) [Effect of picroside II on expressions of TLR4 and NFkappaB in rats with cerebral ischemia reperfusion injury] zhongguo zhong xi yi jie he za zhi zhongguo zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and western medicine/zhongguo zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu Yuan zhu ban 31:58–61
11. Gupta A et al. (2006) Immunomodulatory activity of biopolymeric fraction RLJ-NE-205 from Picrorhiza kurroa. Int Immunopharmacol 6:1543–1549. doi:10.1016/j.intimp.2006.05.002
12. Joy KL, Kuttan R (1999) Anti-diabetic activity of Picrorrhiza kurroa extract. J Ethnopharmacol 67:143–148. doi:10.1016/s0378-8741(98)00243-8
13. Joy KL, Rajeshkumar NV, Kuttan G, Kuttan R (2000) Effect of Picrorrhiza kurroa extract on transplanted tumours and chemical carcinogenesis in mice. J Ethnopharmacol 71:261–266. doi:10.1016/s0378-8741(00)00168-9
14. Kapadia GJ, Sharma SC, Tokuda H, Nishino H, Ueda S (1996) Inhibitory effect of iridoids on epstein-barr virus activation by a short-term in vitro assay for anti-tumor promoters. Cancer Lett 102:223–226
15. Kumar HM et al. (2010) Development of novel lipidated analogs of picroside as vaccine adjuvants: acylated analogs of picroside-II elicit strong Th1 and Th2 response to ovalbumin in mice. Vaccine 28:8327–8337. doi:10.1016/j.vaccine.2010.07.062
16. Lee HS, Ku SK (2008) Effect of picrorrhiza rhizoma extracts on early diabetic nephropathy in streptozotocin-induced diabetic rats. J Med Food 11:294–301. doi:10.1089/jmf.2007.578
17. Lee HS, Kim HH, Ku SK (2008) hepatoprotective effects of artemisiae capillaris herba and picrorrhiza rhizoma combinations on carbon tetrachloride-induced subacute liver damage in rats Nutr Res 28:270–277 doi:10.1016/j.nutres.2008.02.001
18. Medasani M, Divi SB (2011) Picrorhiza kurroa extract for prevention, elimination and treatment of rna viruses and infection or diseases caused by the same in humans, animals and in biotech industry. Patent WO2011027364 A2, March 10, 2011
19. Najar IA, Sachin BS, Sharma SC, Satti NK, Suri KA, Johri RK (2010) Modulation of p-glycoprotein atpase activity by some phytoconstituents. Phytother Res 24:454–458. doi:10.1002/ptr.2951
20. Pandit S, Shitiz K, Sood H, Naik PK, Chauhan RS (2013) Expression pattern of fifteen genes of non-mevalonate (mep) and mevalonate (mva) pathways in different tissues of endangered medicinal herb picrorhiza kurroa with respect to picrosides content. Mol Biol Rep 40:1053–1063. doi:10.1007/s11033-012-2147-1
21. Rajkumar V, Guha G, Kumar RA (2011) Antioxidant and anti-neoplastic activities of picrorhiza kurroa extracts food and chemical toxicology: an international J Published British ind Biol Res Assoc 49:363–369. doi:10.1016/j.fct.2010.11.009
22. Rathee D, Thanki M, Bhuva S, Anandjiwala S, Agrawal R (2013) Iridoid glycosides-kutkin, picroside I, and kutkoside from picrorrhiza kurroa benth inhibits the invasion and migration of MCF-7 breast cancer cells through the down regulation of matrix metalloproteinases. Arab J Chem 6:49–58. doi:10.1016/j.arabjc.2011.01.011
23. Saraswat B, Visen PKS, Patnaik GK, Dhawan BN (1999) Ex vivo and in vivo investigations of picroliv from Picrorhiza kurroa in an alcohol intoxication model in rats. J Ethnopharmacol 66:263–269. doi:10.1016/s0378-8741(99)00007-0
24. Sood H, Chauhan R (2010) Biosynthesis and accumulation of a medicinal compound, picroside-i, in cultures of Picrorhiza kurroa Royle ex Benth. Plant Cell Tissue Organ Cult 100:113–117. doi:10.1007/s11240-009-9618-7
25. Tiwari SS, Pandey MM, Srivastava S, Rawat AK (2012) TLC densitometric quantification of picrosides (picroside-I and picroside-II) in picrorhiza kurroa and its substitute picrorhiza scrophulariiflora and their antioxidant studies. Biomed Chromatogr: BMC 26:61–68. doi:10.1002/bmc.1626