Homology arms of targeting vectors for gene insertions and CRISPR/Cas9 technology: Size does not matter; Quality control of targeted clones does

Cellular and Molecular Biology Letters

Volumn 20, Issue 5, 2015, Pages 773-787

  Petrezselyova, Silvia ^a   Kinsky, Slavomir ^a   Truban, Dominika ^a   Sedlacek, Radislav ^a   Burtscher, Ingo ^b   Lickert, Heiko ^b  

^a INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

^b Arbeitsgruppe NeuroBiologie des Diabetes Institut für Diabetes und Adipositas   (Germany)

Author keywords

CRISPR Cas9; Embryonic stem cell; Genome editing; Homology arms; Reporter; Targeting vector

Indexed keywords

ALLELE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; DNA SEQUENCE; GENE AMPLIFICATION; GENE DISRUPTION; GENE INSERTION; GENE MUTATION; HUMAN; HUMAN EXPERIMENT; KNOCKOUT GENE; MOUSE EMBRYONIC STEM CELL; POLYMERASE CHAIN REACTION; QUALITY CONTROL; SOUTHERN BLOTTING; ANIMAL; C57BL MOUSE; CELL LINE; CRISPR CAS SYSTEM; GENE EXPRESSION REGULATION; GENE TARGETING; GENE VECTOR; GENETICS; METABOLISM; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; STANDARDS;

BASIC HELIX LOOP HELIX TRANSCRIPTION FACTOR; HEPATOCYTE NUCLEAR FACTOR 4; HNF4A PROTEIN, MOUSE; NERVE PROTEIN; NEUROG3 PROTEIN, MOUSE; TCF7L2 PROTEIN, MOUSE; TRANSCRIPTION FACTOR 7 LIKE 2;

ALLELES; ANIMALS; BASE SEQUENCE; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; CELL LINE; CRISPR-CAS SYSTEMS; GENE KNOCK-IN TECHNIQUES; GENETIC VECTORS; HEPATOCYTE NUCLEAR FACTOR 4; MICE; MICE, INBRED C57BL; MOLECULAR SEQUENCE DATA; MOUSE EMBRYONIC STEM CELLS; MUTAGENESIS, INSERTIONAL; NERVE TISSUE PROTEINS; QUALITY CONTROL; TRANSCRIPTION FACTOR 7-LIKE 2 PROTEIN;

EID: 84962588243     PISSN: 14258153     EISSN: 16891392     Source Type: Journal    
DOI: 10.1515/cmble-2015-0047     Document Type: Article

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