Direct identification of urinary tract pathogens from urine samples, combining urine screening methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry

Journal of Clinical Microbiology

Volumn 54, Issue 4, 2016, Pages 988-993

  Íñigo, Melania ^a   Coello, Andreu ^a   Fernández Rivas, Gema ^a   Rivaya, Belén ^a   Hidalgo, Jessica ^a   Quesada, María Dolores ^a   Ausinaa, Vicente ^b  

^a HOSPITAL UNIVERSITARI GERMANS TRIAS I PUJOL   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; AUTOANALYZER; BACTERIUM IDENTIFICATION; CANDIDA; CHROMATOGRAPHY; CITROBACTER FREUNDII; CONTROLLED STUDY; CULTURE MEDIUM; ENTEROBACTER CLOACAE; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ESCHERICHIA COLI; FLOW CYTOMETRY; KLEBSIELLA PNEUMONIAE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROBIAL IDENTIFICATION; MORGANELLA MORGANII; NONHUMAN; PRIORITY JOURNAL; PROTEUS MIRABILIS; PROTEUS VULGARIS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS MENDOCINA; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS SAPROPHYTICUS; STENOTROPHOMONAS MALTOPHILIA; URINALYSIS; URINARY TRACT INFECTION; EVALUATION STUDY; FEMALE; HUMAN; MALE; MASS SCREENING; MATRIX-ASSISTED LASER DESORPTION-IONIZATION MASS SPECTROMETRY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PROCEDURES; URINARY TRACT INFECTIONS; URINE;

FEMALE; HUMANS; MALE; MASS SCREENING; MICROBIOLOGICAL TECHNIQUES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; URINARY TRACT INFECTIONS; URINE;

EID: 84962564916     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02832-15     Document Type: Article

References (24)

1. Flores-Mireles AL, Walker JN, Caparon M, Hultgren SJ. 2015. Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nat Rev Microbiol 13:269-284. http://dx.doi.org/10.1038/nrmicro3432.
2. Andreu A, Planells I, Grupo Cooperativo Español para el Estudio de la Sensibilidad Antimicrobiana de los Patógenos Urinarios. 2008. Etiology of community-acquired lower urinary infections and antimicrobial resistance of Escherichia coli: A national surveillance study. Med Clin (Barc) 130:481-486. (In Spanish.) http://dx.doi.org/10.1157/13119488.
3. McIsaac WJ, Hunchak CL. 2011. Overestimation error and unnecessary antibiotic prescriptions for acute cystitis in adult women. Med Decis Making 31:405-411. http://dx.doi.org/10.1177/0272989X10391671.
4. Lewis JF, Alexander J. 1976. Microscopy of stained urine smears to determine the need for quantitative culture. J Clin Microbiol 4:372-374.
5. Manoni F, Fornasiero L, Ercolin M, Tinello A, Ferrian M, Hoffer P, Valverde S, Gessoni G. 2009. Cutoff values for bacteria and leukocytes for urine flow cytometer Sysmex UF-1000i in urinary tract infections. Diagn Microbiol Infect Dis 65:103-107. http://dx.doi.org/10.1016/j.diagmicrobio.2009.06.003.
6. Karakukcu C, Kayman T, Ozturk A, Torun YA. 2012. Analytic performance of bacteriuria and leukocyturia obtained by UriSed in culture positive urinary tract infections. Clin Lab 58:107-111.
7. Sterry-Blunt RE, Randall SK, Doughton JM, Aliyu HS, Enoch AD. 2015. Screening urine samples for the absence of urinary tract infection using the SediMax automated microscopy analyser. J Med Microbiol 64:605-609. http://dx.doi.org/10.1099/jmm.0.000064.
8. Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D. 2009. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-offlight mass spectrometry. Clin Infect Dis 49:543-551. http://dx.doi.org/10.1086/600885.
9. Ferreira L, Sánchez-Juanes F, González-Avila M, Cembrero-Fuciños D, Herrero-Hernández A, González-Buitrago JM, Muñoz-Bellido JL. 2010. Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48:2110-2115. http://dx.doi.org/10.1128/JCM.02215-09.
10. Stevenson LG, Drake SK, Murray PR. 2010. Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48: 444-447. http://dx.doi.org/10.1128/JCM.01541-09.
11. Wang XH, Zhang G, Fan YY, Yang X, Sui WJ, Lu XX. 2013. Direct identification of bacteria causing urinary tract infections by combining matrix-assisted laser desorption ionization-time of flight mass spectrometry with UF-1000i urine flow cytometry. J Microbiol Methods 92:231-235. http://dx.doi.org/10.1016/j.mimet.2012.12.016.
12. Barnini S, Ghelardi E, Brucculeri V, Morici P, Lupetti A. 2015. Rapid and reliable identification of Gram-negative bacteria and Gram-positive cocci by deposition of bacteria harvested from blood cultures onto the MALDI-TOF plate. BMC Microbiol 15:124. http://dx.doi.org/10.1186/s12866-015-0459-8.
13. March Rosselló GA, Gutiérrez Rodríguez MP, Ortiz de Lejarazu Leonardo R, Orduña Domingo A, Bratos Pérez MA. 2014. New procedure for rapid identification of microorganisms causing urinary tract infection from urine samples by mass spectrometry (MALDI-TOF). Enferm Infecc Microbiol Clin 33:89-94. http://dx.doi.org/10.1016/j.eimc.2014.02.022.
14. Demarco ML, Burnham CA. 2014. Diafiltration MALDI-TOF mass spectrometry method for culture-independent detection and identification of pathogens directly from urine specimens. 141:204-212. Am J Clin Pathol http://dx.doi.org/10.1309/AJCPQYW3B6JLKILC.
15. Altun O, Botero-Kleiven S, Carlsson S, Ullberg M, Ö zenci V. 2015. Rapid identification of bacteria from positive blood culture bottles by MALDI-TOF MS following short-term incubation on solid media. J Med Microbiol 64:1346-1352. http://dx.doi.org/10.1099/jmm.0.000168.
16. Ferreira L, Sánchez-Juanes F, Porras-Guerra I, García-García MI, García-Sánchez JE, González-Buitrago JM, Muñoz-Bellido JL. 2011. Microorganisms direct identification from blood culture by matrixassisted laser desorption/ionization time-of-flight mass spectrometry. Clin Microbiol Infect 17:546-551. http://dx.doi.org/10.1111/j.1469-0691.2010.03257.x.
17. Schmidt V, Jarosch A, März P, Sander C, Vacata V, Kalka-Moll W. 2012. Rapid identification of bacteria in positive blood culture by matrixassisted laser desorption ionization time-of-flight mass spectrometry. Eur J Clin Microbiol Infect Dis 31:311-317. http://dx.doi.org/10.1007/s10096-011-1312-0.
18. Martinez RM, Bauerle ER, Fang FC, Butler-Wu SM. 2014. Evaluation of three rapid diagnostic methods for direct identification of microorganisms in positive blood cultures. J Clin Microbiol 52:2521-2529. http://dx.doi.org/10.1128/JCM.00529-14.
19. Mestas J, Felsenstein S, Bard JD. 2014. Direct identification of bacteria from positive BacT/ALERT blood culture bottles using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. Diagn Microbiol Infect Dis 80:193-196. http://dx.doi.org/10.1016/j.diagmicrobio.2014.07.008.
20. Burillo A, Rodríguez-Sánchez B, Ramiro A, Cercenado E, Rodríguez-Créixems M, Bouza E. 2014. Gram-stain plus MALDI-TOF MS (matrixassisted laser desorption ionization-time of flight mass spectrometry) for a rapid diagnosis of urinary tract infection. PLoS One 9:e86915. http://dx.doi.org/10.1371/journal.pone.0086915.
21. Rodríguez-Sánchez B, Sánchez-Carrillo C, Ruiz A, Marín M, Cercenado E, Rodríguez-Créixems M, Bouza E. 2014. Direct identification of pathogens from positive blood cultures using matrix-assisted laser desorptionionization time-of-flight mass spectrometry. Clin Microbiol Infect 20: O421-O427. http://dx.doi.org/10.1111/1469-0691.12455.
22. Galán F, García-Agudo L, Guerrero I, Marín P, García-Tapia A, García-Martos P, Rodríguez-Iglesias M. 2015. Evaluation of mass spectrometry for the identification of clinically interesting yeasts. Enferm Infecc Microbiol Clin 33:372-378. (In Spanish.) http://dx.doi.org/10.1016/j.eimc.2014.10.003.
23. Sánchez-Juanes F, Siller Ruiz M, Moreno Obregón F, Criado González M, Hernández Egido S, de Frutos Serna M, González-Buitrago JM, Muñoz-Bellido JL. 2014. Pretreatment of urine samples with SDS improves direct identification of urinary tract pathogens with matrixassisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 52:335-338. http://dx.doi.org/10.1128/JCM.01881-13.
24. Veron L, Mailler S, Girard V, Muller BH, L'Hostis G, Ducruix C, Lesenne A, Richez A, Rostaing H, Lanet V, Ghirardi S, van Belkum A, Mallard F. 2015. Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS. Eur J Clin Microbiol Infect Dis 34:1787-1795. http://dx.doi.org/10.1007/s10096-015-2413-y.