Rapid identification of bacteria from positive blood culture bottles by MALDI-TOF MS following short-term incubation on solid media

Journal of Medical Microbiology

Volumn 64, Issue 11, 2015, Pages 1346-1352

  Altun, Osman ^a   Botero Kleiven, Silvia ^a   Carlsson, Sarah ^a   Ullberg, Måns ^a   Özenci, Volkan ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR MEDIUM; AGGLUTINATION TEST; ANAEROBIC BACTERIUM; ARTICLE; BACTERIAL GROWTH; BACTERIUM IDENTIFICATION; BIOCHEMICAL ANALYSIS; BIOCHEMICAL EQUIPMENT; BLOOD CULTURE; CONTROLLED STUDY; CULTURE MEDIUM; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; ENTEROBACTERIACEAE; ENTEROCOCCUS; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; GRAM STAINING; MASS SPECTROMETER; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; SEROLOGY TEST KIT; SPECIES IDENTIFICATION; STAPHYLOCOCCUS; BACTEREMIA; BACTERIUM; BLOOD; CHEMISTRY; CLASSIFICATION; DEVICES; EVALUATION STUDY; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; MICROBIOLOGY; PROCEDURES;

BACTEREMIA; BACTERIA; BACTERIAL TYPING TECHNIQUES; BLOOD; HUMANS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84949036019     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.000168     Document Type: Article

References (24)

1. Beekmann, S. E., Diekema, D. J., Chapin, K. C. & Doern, G. V. (2003). Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J Clin Microbiol 41, 3119-3125.
2. Bhatti, M. M., Boonlayangoor, S., Beavis, K. G. & Tesic, V. (2014). Rapid identification of positive blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry using prewarmed agar plates. J Clin Microbiol 52, 4334-4338.
3. Chapin, K. & Musgnug, M. (2003). Evaluation of three rapid methods for the direct identification of Staphylococcus aureus from positive blood cultures. J Clin Microbiol 41, 4324-4327.
4. Croxatto, A., Prod'hom, G. & Greub, G. (2012). Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology. FEMS Microbiol Rev 36, 380-407.
5. Dark, P., Blackwood, B., Gates, S., McAuley, D., Perkins, G. D., McMullan, R., Wilson, C., Graham, D., Timms, K. & Warhurst, G. (2015). Accuracy of LightCycler(H) SeptiFast for the detection and identification of pathogens in the blood of patients with suspected sepsis: a systematic review and meta-analysis. Intensive Care Med 41, 21-33.
6. Fothergill, A., Kasinathan, V., Hyman, J., Walsh, J., Drake, T. & Wang, Y. F. (2013). Rapid identification of bacteria and yeasts from positiveblood-culture bottles by using a lysis-filtration method and matrixassisted laser desorption ionization-time of flight mass spectrum analysis with the SARAMIS database. J Clin Microbiol 51, 805-809.
7. Goto, M. & Al-Hasan, M. N. (2013). Overall burden of bloodstream infection and nosocomial bloodstream infection in North America and Europe. Clin Microbiol Infect 19, 501-509.
8. Hill, J. T., Tran, K.-D., Barton, K. L., Labreche, M. J. & Sharp, S. E. (2014). Evaluation of the nanosphere Verigene BC-GN assay for direct identification of Gram-negative bacilli and antibiotic resistance markers from positive blood cultures and potential impact for morerapid antibiotic interventions. J Clin Microbiol 52, 3805-3807.
9. Idelevich, E. A., Schüle, I., Grünastel, B., Wüllenweber, J., Peters, G. & Becker, K. (2014a). Acceleration of antimicrobial susceptibility testing of positive blood cultures by inoculation of Vitek 2 cards with briefly incubated solid medium cultures. J Clin Microbiol 52, 4058-4062.
10. Idelevich, E. A., Schüle, I., Grünastel, B., Wüllenweber, J., Peters, G. & Becker, K. (2014b). Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium. Clin Microbiol Infect 20, 1001-1006.
11. Judd, W. R., Stephens, D. M. & Kennedy, C. A. (2014). Clinical and economic impact of a quality improvement initiative to enhance early recognition and treatment of sepsis. Ann Pharmacother 48, 1269-1275.
12. Kang, C.-I., Kim, S.-H., Park, W.-B., Lee, K.-D., Kim, H.-B., Kim, E.-C., Oh, M. D. & Choe, K.-W. (2005). Bloodstream infections caused by antibiotic-resistant Gram-negative bacilli: risk factors for mortality and impact of inappropriate initial antimicrobial therapy on outcome. Antimicrob Agents Chemother 49, 760-766.
13. Kollef, M. H. (2008). Broad-spectrum antimicrobials and the treatment of serious bacterial infections: getting it right up front. Clin Infect Dis 47 (Suppl. 1), S3-S13.
14. Kothari, A., Morgan, M. & Haake, D. A. (2014). Emerging technologies for rapid identification of bloodstream pathogens. Clin Infect Dis 59, 272-278.
15. Kumar, A., Roberts, D., Wood, K. E., Light, B., Parrillo, J. E., Sharma, S., Suppes, R., Feinstein, D., Zanotti, S. & other authors (2006). Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 34, 1589-1596.
16. Martinez, R. M., Bauerle, E. R., Fang, F. C. & Butler-Wu, S. M. (2014). Evaluation of three rapid diagnostic methods for direct identification of microorganisms in positive blood cultures. J Clin Microbiol 52, 2521-2529.
17. Neville, S. A., Lecordier, A., Ziochos, H., Chater, M. J., Gosbell, I. B., Maley, M. W. & van Hal, S. J. (2011). Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification. J Clin Microbiol 49, 2980-2984.
18. Özenci, V., Tegmark-Wisell, K., Lundberg, C. & Wretlind, B. (2008). Rapid culture and identification: a practical method for early preliminary laboratory diagnosis of sepsis. Clin Microbiol Infect 14, 177-180.
19. Peters, R. P., van Agtmael, M. A., Simoons-Smit, A. M., Danner, S. A., Vandenbroucke-Grauls, C. M. & Savelkoul, P. H. (2006). Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay. J ClinMicrobiol 44, 4186-4188.
20. Petti, C. A., Woods, C. W. & Reller, L. B. (2005). Streptococcus pneumoniae antigen test using positive blood culture bottles as an alternative method to diagnose pneumococcal bacteremia. J Clin Microbiol 43, 2510-2512.
21. Schulthess, B., Brodner, K., Bloemberg, G. V., Zbinden, R., Böttger, E. C. & Hombach, M. (2013). Identification of Gram-positive cocci by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry: comparison of different preparation methods and implementation of a practical algorithm for routine diagnostics. J Clin Microbiol 51, 1834-1840.
22. Verroken, A., Defourny, L., Lechgar, L., Magnette, A., Delmée, M. & Glupczynski, Y. (2015). Reducing time to identification of positive blood cultures with MALDI-TOF MS analysis after a 5-h subculture. Eur J Clin Microbiol Infect Dis 34, 405-413.
23. Vlek, A. L. M., Bonten, M. J. M. & Boel, C. H. E. (2012). Direct matrixassisted laser desorption ionization time-of-flight mass spectrometry improves appropriateness of antibiotic treatment of bacteremia. PLoS One 7, e32589.
24. Wüppenhorst, N., Consoir, C., Lörch, D. & Schneider, C. (2012). Direct identification of bacteria from charcoal-containing blood culture bottles using matrix-assisted laser desorption/ionisation time-offlight mass spectrometry. Eur J Clin Microbiol Infect Dis 31, 2843-2850.