Identification and characterization of a novel bifunctional Δ12/Δ15-fatty acid desaturase gene from Rhodosporidium kratochvilovae

Biotechnology Letters

Volumn 38, Issue 7, 2016, Pages 1155-1164

  Cui, Jinjin ^a   He, Shiwu ^a   Ji, Xiuling ^a   Lin, Lianbing ^a   Wei, Yunlin ^a   Zhang, Qi ^a  

^a KUNMING UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Cold adaptation; Polyunsaturated fatty acids; Rhodosporidium kratochvilovae; Substrate specificity; 12 Fatty acid desaturase; 15 Fatty acid desaturase

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; ENCODING (SYMBOLS); GENE EXPRESSION; POLYUNSATURATED FATTY ACIDS; SIGNAL ENCODING; TEMPERATURE; YEAST;

ALPHA LINOLENIC ACIDS; BIOSYNTHESIS PATHWAYS; COLD ADAPTATION; DESATURASE; FUNCTIONAL CHARACTERIZATION; MRNA EXPRESSION LEVEL; RHODOSPORIDIUM KRATOCHVILOVAE; SUBSTRATE SPECIFICITY;

LINOLEIC ACID;

ACYL COENZYME A DESATURASE; FATTY ACID; LINOLENIC ACID; UNSATURATED FATTY ACID;

CLASSIFICATION; COLD; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; METABOLISM; PHYLOGENY; PHYSIOLOGY; RHODOSPIRILLUM;

ALPHA-LINOLENIC ACID; COLD TEMPERATURE; FATTY ACID DESATURASES; FATTY ACIDS; FATTY ACIDS, UNSATURATED; PHYLOGENY; RHODOSPIRILLUM; SUBSTRATE SPECIFICITY;

EID: 84962028781     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-016-2090-7     Document Type: Article

References (40)

1. Ageitos JM, Vallejo JA, Veiga-Crespo P, Villa TG (2011) Oily yeasts as oleaginous cell factories. Appl Microbiol Biotechnol 90:1219–1227
2. Aitzetmuller K, Tsevegsuren N (1994) Seed fatty acids, «Front-End»-desaturases and chemotaxonomy—a case study in the Ranunculaceae. J Plant Physiol 143:538–543
3. Alonso DL, Garcia-Maroto F, Rodriguez-Ruiz J, Garrido JA, Vilches MA (2003) Evolution of the membrane-bound fatty acid desaturases. Biochem Syst Ecol 31:1111–1124
4. Beopoulos A, Cescut J, Haddouche R, Uribelarrea JL, Molina-Jouve C, Nicaud JM (2009) Yarrowia lipolytica as a model for bio-oil production. Prog Lipid Res 48:375–387
5. Bucek A, Matouskova P, Sychrova H, Pichova I, Hruskova-Heidingsfeldova O (2014) Δ12-fatty acid desaturase from Candida parapsilosis is a multifunctional desaturase producing a range of polyunsaturated and hydroxylated fatty acids. PLoS One 9:e93322
6. Certik M, Shimizu S (1999) Biosynthesis and regulation of microbial polyunsaturated fatty acid production. J Biosci Bioeng 87:1–14
7. Chintalapati S, Kiran MD, Shivaji S (2004) Role of membrane lipid fatty acids in cold adaptation. Cell Mol Biol 50:631–642
8. Damude HG, Zhang HX, Farrall L, Ripp KG, Tomb JF, Hollerbach D, Yadav NS (2006) Identification of bifunctional Δ12/ω3 fatty acid desaturases for improving the ratio of ω3 to ω6 fatty acids in microbes and plants. Proc Natl Acad Sci USA 103:9446–9451
9. Elble R (1992) A simple and efficient procedure for transformation of yeasts. Biotechniques 13:18–20
10. Gill I, Valivety R (1997) Polyunsaturated fatty acids, part 1: occurrence, biological activities and applications. Trends Biotechnol 15:401–409
11. Hallahan B, Garland MR (2005) Essential fatty acids and mental health. Brit J Psychiatr 186:275–277
12. Hoffmann M, Hornung E, Busch S, Kassner N, Ternes P, Braus GH, Feussner I (2007) A small membrane-peripheral region close to the active center determines regioselectivity of membrane-bound fatty acid desaturases from Aspergillus nidulans. J Biol Chem 282:26666–26674
13. Huang YS, Chaudhary S, Thurmond JM, Bobik EG Jr, Yuan L, Chan GM, Kirchner SJ, Mukerji P, Knutzon DS (1999) Cloning of Δ12- and Δ6-desaturases from Mortierella alpina and recombinant production of γ-linolenic acid in Saccharomyces cerevisiae. Lipids 34:649–659
14. Kawamoto J, Kurihara T, Yamamoto K, Nagayasu M, Tani Y, Mihara H, Hosokawa M, Baba T, Sato SB, Esaki N (2009) Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium, Shewanella livingstonensis Ac10. J Bacteriol 191:632–640
15. Li YH, Zhao ZB, Bai FW (2007) High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture. Enzyme Microb Technol 41:312–317
16. 8-sphingolipid desaturases. Biochem Biophys Res Commun 279:779–785
17. −ΔΔCT method. Methods 25:402–408
18. Los DA, Murata N (1998) Structure and expression of fatty acid desaturases. Biochim Biophys Acta 1394:3–15
19. Meesapyodsuk D, Qiu X (2012) The front-end desaturase: structure, function, evolution and biotechnological use. Lipids 47:227–237
20. Meesapyodsuk D, Reed DW, Covello PS, Qiu X (2007) Primary structure, regioselectivity, and evolution of the membrane-bound fatty acid desaturases of Claviceps purpurea. J Biol Chem 282:20191–20199
21. Ntambi JM, Bene H (2001) Polyunsaturated fatty acid regulation of gene expression. J Mol Neurosci 16:273–278 discussion 279-284
22. 12-desaturase gene in Saccharomyces cerevisiae. Biochem Biophys Res Commun 263:47–51
23. Pereira SL, Leonard AE, Mukerji P (2003) Recent advances in the study of fatty acid desaturases from animals and lower eukaryotes. Prostaglandin Leukot Essent 68:97–106
24. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
25. Sakai H, Kajiwara S (2005) Cloning and functional characterization of a Δ12 fatty acid desaturase gene from the basidiomycete Lentinula edodes. Mol Genet Genom 273:336–341
26. Sakamoto T, Los DA, Higashi S, Wada H, Nishida I, Ohmori M, Murata N (1994) Cloning of ω3 desaturase from cyanobacteria and its use in altering the degree of membrane-lipid unsaturation. Plant Mol Biol 26:249–263
27. Sayanova O, Haslam R, Guschina I, Lloyd D, Christie WW, Harwood JL, Napier JA (2006) A bifunctional Δ12, Δ15-desaturase from Acanthamoeba castellanii directs the synthesis of highly unusual n-1 series unsaturated fatty acids. J Biol Chem 281:36533–36541
28. Sprecher H, Luthria DL, Mohammed BS, Baykousheva SP (1995) Reevaluation of the pathways for the biosynthesis of polyunsaturated fatty acids. J Lipid Res 36:2471–2477
29. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
30. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acid Res 25:4876–4882
31. Tu WC, Cook-Johnson RJ, James MJ, Muhlhausler BS, Gibson RA (2010) Omega-3 long chain fatty acid synthesis is regulated more by substrate levels than gene expression. Prostaglandins Leukot Essent 83:61–68
32. Uemura H (2012) Synthesis and production of unsaturated and polyunsaturated fatty acids in yeast: current state and perspectives. Appl Microbiol Biotechnol 95:1–12
33. Wada H, Gombos Z, Murata N (1990) Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation. Nature 347:200–203
34. Wang MX, Chen HQ, Gu ZN, Zhang H, Chen W, Chen YQ (2013) ω3 fatty acid desaturases from microorganisms: structure, function, evolution, and biotechnological use. Appl Microbiol Biotechnol 97:10255–10262
35. Watanabe T, Tsuda S, Nishimura H, Honda Y, Watanabe T (2010) Characterization of a Δ12-fatty acid desaturase gene from Ceriporiopsis subvermispora, a selective lignin-degrading fungus. Appl Microbiol Biotechnol 87:215–224
36. 12-fatty acid desaturase gene from Rhizopus arrhizus. FEBS Lett 573:45–50
37. 12-fatty acid desaturase gene from methylotrophic yeast Pichia pastoris GS115. Acta Biochim Pol 53:753–759
38. Weinstein RN, Montiel PO, Johnstone K (2000) Influence of growth temperature on lipid and soluble carbohydrate synthesis by fungi isolated from fellfield soil in the maritime Antarctic. Mycologia 92:222–229
39. Zhang S, Sakuradani E, Ito K, Shimizu S (2007) Identification of a novel bifunctional Δ12/Δ15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD#822-2. FEBS Lett 581:315–319
40. Yan Z, Zhuo L, Mulan J, Xia W, YangminZ G, Yinbo Z, Fenghong H (2013) Clone and identification of bifunctional Δ12/Δ15 fatty acid desaturase LKFAD15 from Lipomyces kononenkoae. Food Sci Biotechnol 22:573–576