Simultaneous Determination of Baicalein and Baicalin in Human Plasma by High Performance Liquid Chromatograph-Tandem Spectrometry and its Application in a Food-Effect Pharmacokinetic Study

Drug Research

Volumn 66, Issue 8, 2016, Pages 394-401

  Pang, H ^a,b   Shi, A ^b   Li, M ^b   Xue, W ^b   Li, Y ^b   Cao, G ^b   Yan, B ^b   Dong, F ^b   Xiao, W ^c   He, G ^d   Du, G ^d   Hu, X ^b   Cheng, G ^a  

^a SHENYANG PHARMACEUTICAL UNIVERSITY   (China)

^b BEIJING HOSPITAL   (China)

^c JIANGSU KANION PHARMACEUTICAL CO   (China)

^d INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

incurred samples reanalysis; liquid liquid extraction; positive electrospray ionization

Indexed keywords

ACETONITRILE; AMMONIUM ACETATE; BAICALEIN; BAICALIN; FORMIC ACID; METHANOL; PLACEBO; FLAVANONE DERIVATIVE; FLAVONOID; TABLET;

ADULT; AQUEOUS SOLUTION; AREA UNDER THE CURVE; ARTICLE; CALIBRATION; CHEWABLE TABLET; CONTROLLED STUDY; CROSSOVER PROCEDURE; DEATH; DRUG BLOOD LEVEL; DRUG DETERMINATION; DRUG FATALITY; DRUG HALF LIFE; DRUG SAFETY; DRUG STABILITY; DRUG STORAGE; DRUG TOLERABILITY; FEMALE; FOOD DRUG INTERACTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN EXPERIMENT; LIMIT OF DETECTION; LIMIT OF QUANTITATION; LIQUID LIQUID EXTRACTION; MALE; MASS SPECTROMETER; MAXIMUM PLASMA CONCENTRATION; MEASUREMENT ACCURACY; MEASUREMENT PRECISION; MONITORING; MULTIPLE REACTION MONITORING; NORMAL HUMAN; NORMAL VALUE; POSITIVE ION ELECTROSPRAY; QUADRUPOLE MASS SPECTROMETRY; QUALITY CONTROL; RANDOMIZED CONTROLLED TRIAL; SINGLE DRUG DOSE; TANDEM MASS SPECTROMETRY; TIME TO MAXIMUM PLASMA CONCENTRATION; UNSPECIFIED SIDE EFFECT; VALIDATION STUDY; BLOOD; DIET RESTRICTION; ELECTROSPRAY MASS SPECTROMETRY; ORAL DRUG ADMINISTRATION; PROCEDURES; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TABLET;

ADMINISTRATION, ORAL; CALIBRATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DRUG STABILITY; FASTING; FEMALE; FLAVANONES; FLAVONOIDS; FOOD-DRUG INTERACTIONS; HUMANS; LIQUID-LIQUID EXTRACTION; MALE; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TABLETS; TANDEM MASS SPECTROMETRY;

EID: 84961886836     PISSN: 21949379     EISSN: 21949387     Source Type: Journal    
DOI: 10.1055/s-0035-1569446     Document Type: Article

References (26)

1. Lin C. C., Shieh D. E. The anti-inflammatory activity of Scutellaria rivularis extracts and its active components, baicalin, baicalein and wogonin. Am J Chin Med: 1996; 24 31 36
2. Kong E. K., Yu S., Sanderson J. E. et al. A novel anti-fibrotic agent, baicalein, for the treatment of myocardial fibrosis in spontaneously hypertensive rats. Eur J Pharmacol: 2011; 658 175 181
3. Dou J., Chen L., Xu G. et al. Effects of baicalein on Sendai virus in vivo are linked to serum baicalin and its inhibition of hemagglutinin-neuraminidase. Arch Virol: 2011; 156 793 801
4. Markovic Z. S., Dimitric Markovic J. M., Milenkovic D. et al. Mechanistic study of the structure-activity relationship for the free radical scavenging activity of baicalein. J Mol Model: 2011; 17 2575 2584
5. Lee Y. M., Cheng P. Y., Chim L. S. et al. Baicalein, an active component of Scutellaria baicalensis Georgi, improves cardiac contractile function in endotoxaemic rats via induction of heme oxygenase-1 and suppression of inflammatory responses. Journal of Ethnopharmacology: 2011; 135 179 185
6. Cheng Y., He G., Mu X. et al. Neuroprotective effect of baicalein against MPTP neurotoxicity: behavioral, biochemical and immunohistochemical profile. Neurosci Lett: 2008; 441 16 20
7. Mu X., He G., Cheng Y. et al. Baicalein exerts neuroprotective effects in 6-hydroxydopamine-induced experimental parkinsonism in vivo and in vitro. Pharmacol Biochem Behav: 2009; 92 642 648
8. Mu X., He G. R., Yuan X. et al. Baicalein protects the brain against neuron impairments induced by MPTP in C57BL/6 mice. Pharmacol Biochem Behav: 2011; 98 286 291
9. Wang Y. H., Yu H. T., Pu X. P. et al. Baicalein prevents 6-hydroxydopamine-induced mitochondrial dysfunction in SH-SY5Y cells via inhibition of mitochondrial oxidation and up-regulation of DJ-1 protein expression. Molecules: 2013; 18 14726 14738
10. Taiming L., Xuehua J. Investigation of the absorption mechanisms of baicalin and baicalein in rats. J Pharm Sci: 2006; 95 1326 1333
11. Lai M. Y., Hsiu S. L., Tsai S. Y. et al. Comparison of metabolic pharmacokinetics of baicalin and baicalein in rats. J Pharm Pharmacol: 2003; 55 205 209
12. Zhang L., Lin G., Zuo Z. High-performance liquid chromatographic method for simultaneous determination of baicalein and baicalein 7-glucuronide in rat plasma. Journal of Pharmaceutical and Biomedical Analysis: 2004; 36 637 641
13. Li C., Zhang L., Lin G. et al. Identification and quantification of baicalein, wogonin, oroxylin A and their major glucuronide conjugated metabolites in rat plasma after oral administration of Radix scutellariae product. Journal of Pharmaceutical and Biomedical Analysis: 2011; 54 750 758
14. Tian S., Du L., Wang S. et al. Pharmacokinetic study of baicalein and its major metabolites after iv administration in dogs. Chinese herbal medicine: 2011; 196 201
15. Tian S., He G., Song J. et al. Pharmacokinetic study of baicalein after oral administration in monkeys. Fitoterapia: 2012; 83 532 540
16. Wakui Y., Yanagisawa E., Ishibashi E. et al. Determination of baicalin and baicalein in rat plasma by high-performance liquid chromatography with electrochemical detection. Journal of chromatography B Analyt Technol Biomed Life Sci: 1992; 575 131 136
17. Feng J., Xu W., Tao X. et al. Simultaneous determination of baicalin, baicalein, wogonin, berberine, palmatine and jatrorrhizine in rat plasma by liquid chromatography-tandem mass spectrometry and application in pharmacokinetic studies after oral administration of traditional Chinese medicinal preparations containing scutellaria-coptis herb couple. Journal of Pharmaceutical and Biomedical Analysis: 2010; 53 591 598
18. Yang R., Zeng H. J., Wang Q. W. et al. Simultaneous determination of eight active components in Chinese medicine 'JiangYaBiFeng' tablet by HPLC coupled with diode array detection. Journal of Pharmaceutical and Biomedical Analysis: 2011; 55 552 556
19. Zeng L., Wang M., Yuan Y. et al. Simultaneous multi-component quantitation of Chinese herbal injection Yin-zhi-huang in rat plasma by using a single-tube extraction procedure for mass spectrometry-based pharmacokinetic measurement. Journal of chromatography B, Analytical technologies in the biomedical and life sciences: 2014; 967 245 254
20. Hou Y. C., Lin S. P., Tsai S. Y. et al. Flavonoid pharmacokinetics and tissue distribution after repeated dosing of the roots of Scutellaria baicalensis in rats. Planta Med: 2011; 77 455 460
21. Tong L., Wan M., Zhang L. et al. Simultaneous determination of baicalin, wogonoside, baicalein, wogonin, oroxylin A and chrysin of Radix scutellariae extract in rat plasma by liquid chromatography tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis: 2012; 70 6 12
22. Fong S. Y., Wong Y. C., Zuo Z. Development of a SPE-LC/MS/MS method for simultaneous quantification of baicalein, wogonin, oroxylin A and their glucuronides baicalin, wogonoside and oroxyloside in rats and its application to brain uptake and plasma pharmacokinetic studies. Journal of Pharmaceutical and Biomedical Analysis: 2014; 97 9 23
23. Zhang L., Lin G., Chang Q. et al. Role of intestinal first-pass metabolism of baicalein in its absorption process. Pharmaceutical Research: 2005; 22 1050 1058
24. Zhang L., Lin G., Zuo Z. UDP-glucuronosyltransferases in the extensive liver and intestinal first-pass metabolism of flavonoid baicalein. Pharmaceutical Research: 2007; 24 81 89
25. Li M., Shi A., Pang H. et al. Safety, tolerability, and pharmacokinetics of a single ascending dose of baicalein chewable tablets in healthy subjects. Journal of Ethnopharmacology: 2014; 156 210 215
26. Saif M. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. Jama: 2000; 284 3043 3045