Development of a multiplex real-time PCR for the simultaneous detection of herpes simplex and varicella zoster viruses in cerebrospinal fluid and lesion swab specimens

Journal of Virological Methods

Volumn 229, Issue , 2016, Pages 16-23

  Wong, Anita A ^a   Pabbaraju, Kanti ^a   Wong, Sallene ^a   Tellier, Raymond ^a,b  

^a PROVINCIAL LABORATORY FOR PUBLIC HEALTH   (Canada)

^b UNIVERSITY OF CALGARY   (Canada)

Author keywords

Cerebral spinal fluid; Herpes simplex virus; Lesion; Multiplex; Real time PCR; Varicella zoster virus

Indexed keywords

ARTICLE; CANADA; CEREBROSPINAL FLUID; CONTROLLED STUDY; HERPES SIMPLEX VIRUS 1; HERPES SIMPLEX VIRUS 2; MEASUREMENT ACCURACY; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; PROSPECTIVE STUDY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VARICELLA ZOSTER VIRUS; VIRUS CULTURE; VIRUS DETECTION; ALBERTA; CHICKENPOX; ENCEPHALITIS, VIRAL; HERPES SIMPLEX; HUMAN; ISOLATION AND PURIFICATION; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; SIMPLEXVIRUS; SKIN; VIROLOGY;

ALBERTA; CANADA; CEREBROSPINAL FLUID; CHICKENPOX; ENCEPHALITIS, VIRAL; HERPES SIMPLEX; HERPESVIRUS 3, HUMAN; HUMANS; MULTIPLEX POLYMERASE CHAIN REACTION; PROSPECTIVE STUDIES; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SIMPLEXVIRUS; SKIN;

EID: 84960844419     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2015.12.009     Document Type: Article

References (45)

1. Aberle S.W., Aberle J.H., Steininger C., Puchhammer-Stockl E. Quantitative real time PCR detection of Varicella-zoster virus DNA in cerebrospinal fluid in patients with neurological disease. Med. Microbiol. Immunol. 2005, 194:7-12. 10.1007/s00430-003-0202-1.
2. Aldea C., Alvarez C.P., Folgueira L., Delgado R., Otero J.R. Rapid detection of herpes simplex virus DNA in genital ulcers by real-time PCR using SYBR green I dye as the detection signal. J. Clin. Microbiol. 2002, 40:1060-1062.
3. Berrington W.R., Jerome K.R., Cook L., Wald A., Corey L., Casper C. Clinical correlates of herpes simplex virus viremia among hospitalized adults. Clin. Infect. Dis. 2009, 49:1295-1301. 10.1086/606053.
4. Birch C.J., Druce J.D., Catton M.C., MacGregor L., Read T. Detection of varicella zoster virus in genital specimens using a multiplex polymerase chain reaction. Sex. Transm. Infect. 2003, 79:298-300.
5. Bradley H., Markowitz L.E., Gibson T., McQuillan G.M. Seroprevalence of herpes simplex virus types 1 and 2 - United States, 1999-2010. J. Infect. Dis. 2014, 209:325-333. 10.1093/infdis/jit458.
6. Calvario A., Bozzi A., Scarasciulli M., Ventola C., Seccia R., Stomati D., Brancasi B. Herpes Consensus PCR test: a useful diagnostic approach to the screening of viral diseases of the central nervous system. J. Clin. Virol. 2002, 25(Suppl. 1):S71-S78. S1386653202000367.
7. Cardenas A.M., Edelstein P.H., Alby K. Development and optimization of a real-time PCR assay for detection of herpes simplex and varicella-zoster viruses in skin and mucosal lesions by use of the BD max open system. J. Clin. Microbiol. 2014, 52:4375-4376. 10.1128/JCM.02237-14.
8. Cone R.W., Hobson A.C., Brown Z., Ashley R., Berry S., Winter C., Corey L. Frequent detection of genital herpes simplex virus DNA by polymerase chain reaction among pregnant women. JAMA 1994, 272:792-796.
9. Corey L., Huang M.L., Selke S., Wald A. Differentiation of herpes simplex virus types 1 and 2 in clinical samples by a real-time taqman PCR assay. J. Med. Virol. 2005, 76:350-355.
10. DeBiasi R.L., Kleinschmidt-DeMasters B.K., Weinberg A., Tyler K.L. Use of PCR for the diagnosis of herpesvirus infections of the central nervous system. J. Clin. Virol. 2002, 25(Suppl. 1):S5-S11. S1386653202000288.
11. Engelmann I., Petzold D.R., Kosinska A., Hepkema B.G., Schulz T.F., Heim A. Rapid quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6 DNA in blood and other clinical specimens. J. Med. Virol. 2008, 80:467-477. 10.1002/jmv.21095.
12. Espy M.J., Uhl J.R., Mitchell P.S., Thorvilson J.N., Svien K.A., Wold A.D., Smith T.F. Diagnosis of herpes simplex virus infections in the clinical laboratory by LightCycler PCR. J. Clin. Microbiol. 2000, 38:795-799.
13. Fan F., Stiles J., Mikhlina A., Lu X., Babady N.E., Tang Y.W. Clinical validation of the Lyra direct HSV 1+2/VZV assay for simultaneous detection and differentiation of three herpesviruses in cutaneous and mucocutaneous lesions. J. Clin. Microbiol. 2014, 52:3799-3801. 10.1128/JCM.02098-14.
14. Forward K.R., Lee S.H. Predominance of herpes simplex virus type 1 from patients with genital herpes in Nova Scotia. Can. J. Infect. Dis. 2003, 14:94-96.
15. Gilbert M., Li X., Petric M., Krajden M., Isaac-Renton J.L., Ogilvie G., Rekart M.L. Using centralized laboratory data to monitor trends in herpes simplex virus type 1 and 2 infection in British Columbia and the changing etiology of genital herpes. Can. J. Public Health 2011, 102:225-229.
16. Gilden D., Cohrs R.J., Mahalingam R., Nagel M.A. Neurological disease produced by varicella zoster virus reactivation without rash. Curr. Top. Microbiol. Immunol. 2010, 342:243-253. 10.1007/82_2009_3.
17. Gilden D.H., Mahalingam R., Cohrs R.J., Tyler K.L. Herpesvirus infections of the nervous system. Nat. Clin. Pract. Neurol. 2007, 3:82-94. 10.1038/ncpneuro0401.
18. Grant R.M., Weitzman S.S., Sherman C.G., Sirkin W.L., Petric M., Tellier R. Fulminant disseminated varicella zoster virus infection without skin involvement. J. Clin. Virol. 2002, 24:7-12. S1386653201002177.
19. Harel L., Smetana Z., Prais D., Book M., Alkin M., Supaev E., Mendelson E., Amir J. Presence of viremia in patients with primary herpetic gingivostomatitis. Clin. Infect. Dis. 2004, 39:636-640. CID33066. 10.1086/422643.
20. Herget G.W., Riede U.N., Schmitt-Graff A., Lubbert M., Neumann-Haefelin D., Kohler G. Generalized herpes simplex virus infection in an immunocompromised patient - report of a case and review of the literature. Pathol. Res. Pract. 2005, 201:123-129. S0344-0338(05)00004-X. 10.1016/j.prp.2004.12.003.
21. Hobson-Peters J., O'loughlin P., Toye P. Development of an internally controlled, homogeneous polymerase chain reaction assay for the simultaneous detection and discrimination of herpes simplex virus types 1 and 2 and varicella-zoster virus. Mol. Cell. Probes 2007, 21:24-30.
22. Ihekwaba U.K., Kudesia G., McKendrick M.W. Clinical features of viral meningitis in adults: significant differences in cerebrospinal fluid findings among herpes simplex virus, varicella zoster virus, and enterovirus infections. Clin. Infect. Dis. 2008, 47:783-789. 10.1086/591129.
23. Jerome K.R., Huang M.L., Wald A., Selke S., Corey L. Quantitative stability of DNA after extended storage of clinical specimens as determined by real-time PCR. J. Clin. Microbiol. 2002, 40:2609-2611.
24. Johnson G., Ayers M., McClure S.C., Richardson S.E., Tellier R. Detection and identification of Bartonella species pathogenic for humans by PCR amplification targeting the riboflavin synthase gene (ribC). J. Clin. Microbiol. 2003, 41:1069-1072.
25. Johnston C., Magaret A., Selke S., Remington M., Corey L., Wald A. Herpes simplex virus viremia during primary genital infection. J. Infect. Dis. 2008, 198:31-34. 10.1086/588676.
26. Kalpoe J.S., Kroes A.C., Verkerk S., Claas E.C., Barge R.M., Beersma M.F. Clinical relevance of quantitative varicella-zoster virus (VZV) DNA detection in plasma after stem cell transplantation. Bone Marrow Transplant. 2006, 38:41-46. 10.1038/sj.bmt.1705397.
27. Kierat S., Les K., Przybylski M., Dzieciatkowski T., Mlynarczyk G. [TaqMan fluorescent probe-based real-time PCR assay for detection of varicella-zoster virus]. Med. Dosw. Mikrobiol. 2012, 64:139-149.
28. Lang A.L., Roberts C., Mazzulli T., Hatchette T.F., LeBlanc J.J. Detection and differentiation of herpes simplex viruses by use of the viper platform: advantages, limitations, and concerns. J. Clin. Microbiol. 2014, 52:2186-2188. 10.1128/JCM.03636-13.
29. Leland D.S., Ginocchio C.C. Role of cell culture for virus detection in the age of technology. Clin. Microbiol. Rev. 2007, 20:49-78.
30. Leveque N., Van H.A., Renois F., Boutolleau D., Talmud D., Andreoletti L. Rapid virological diagnosis of central nervous system infections by use of a multiplex reverse transcription-PCR DNA microarray. J. Clin. Microbiol. 2011, 49:3874-3879.
31. Melvin A.J., Mohan K.M., Schiffer J.T., Drolette L.M., Magaret A., Corey L., Wald A. Plasma and cerebrospinal fluid herpes simplex virus levels at diagnosis and outcome of neonatal infection. J. Pediatr. 2015, 166:827-833. S0022-3476(14)01063-4. 10.1016/j.jpeds.2014.11.011.
32. Montalbano M., Slapak-Green G.I., Neff G.W. Fulminant hepatic failure from herpes simplex virus: post liver transplantation acyclovir therapy and literature review. Transplant. Proc. 2005, 37:4393-4396. S0041-1345(05)01157-7. 10.1016/j.transproceed.2005.10.031.
33. Pereira V.S., Moizeis R.N., Fernandes T.A., Araujo J.M., Meissner R.V., Fernandes J.V. Herpes simplex virus type 1 is the main cause of genital herpes in women of Natal, Brazil. Eur. J. Obstet. Gynecol. Reprod. Biol. 2012, 161:190-193. S0301-2115(11)00660-9. 10.1016/j.ejogrb.2011.12.006.
34. Pillet S., Verhoeven P.O., Epercieux A., Bourlet T., Pozzetto B. Development and validation of a laboratory-developed multiplex real-time PCR assay on the BD max system for detection of herpes simplex virus and varicella-zoster virus DNA in various clinical specimens. J. Clin. Microbiol. 2015, 53:1921-1926. 10.1128/JCM.03692-14.
35. Reil H., Bartlime A., Drerup J., Grewing T., Korn K. Clinical validation of a new triplex real-time polymerase chain reaction assay for the detection and discrimination of Herpes simplex virus types 1 and 2. J. Mol. Diagn. 2008, 10:361-367.
36. Rubben A., Baron J.M., Grussendorf-Conen E.I. Routine detection of herpes simplex virus and varicella zoster virus by polymerase chain reaction reveals that initial herpes zoster is frequently misdiagnosed as herpes simplex. Br. J. Dermatol. 1997, 137:259-261.
37. Sankuntaw N., Sukprasert S., Engchanil C., Kaewkes W., Chantratita W., Pairoj V., Lulitanond V. Single tube multiplex real-time PCR for the rapid detection of herpesvirus infections of the central nervous system. Mol. Cell. Probes 2011, 25:114-120.
38. Sauerbrei A., Wutzler P. Laboratory diagnosis of central nervous system infections caused by herpesviruses. J. Clin. Virol. 2002, 25(Suppl. 1):S45-S51. S1386653202000331.
39. Schloss L., van Loon A.M., Cinque P., Cleator G., Echevarria J.M., Falk K.I., Klapper P., Schirm J., Vestergaard B.F., Niesters H., Popow-Kraupp T., Quint W., Linde A. An international external quality assessment of nucleic acid amplification of herpes simplex virus. J. Clin. Virol. 2003, 28:175-185. S1386653203000039.
40. Schmutzhard J., Merete R.H., Zweygberg W.B., Grillner L. Detection of herpes simplex virus type 1, herpes simplex virus type 2 and varicella-zoster virus in skin lesions, Comparison of real-time PCR, nested PCR and virus isolation. J. Clin. Virol. 2004, 29:120-126.
41. Stocher M., Holzl G., Stekel H., Berg J. Automated detection of five human herpes virus DNAs by a set of LightCycler PCRs complemented with a single multiple internal control. J. Clin. Virol. 2004, 29:171-178. S1386653203001215. 10.1016/S1386-6532(03)00121-5.
42. Tanaka T., Kogawa K., Sasa H., Nonoyama S., Furuya K., Sato K. Rapid and simultaneous detection of 6 types of human herpes virus (herpes simplex virus, varicella-zoster virus, Epstein-Barr virus, cytomegalovirus, human herpes virus 6A/B, and human herpes virus 7) by multiplex PCR assay. Biomed. Res. 2009, 30:279-285. 10.2220/biomedres/30.279.
43. Tang J.W., Lin M., Chiu L., Koay E.S. Viral loads of herpes simplex virus in clinical samples - a 5-year retrospective analysis. J. Med. Virol. 2010, 82:1911-1916. 10.1002/jmv.21903.
44. Wilson D.A., Yen-Lieberman B., Schindler S., Asamoto K., Schold J.D., Procop G.W. Should varicella-zoster virus culture be eliminated? A comparison of direct immunofluorescence antigen detection, culture, and PCR, with a historical review. J. Clin. Microbiol. 2012, 50:4120-4122.
45. Zheng Z.B., Wu Y.D., Yu X.L., Shang S.Q. DNA microarray technology for simultaneous detection and species identification of seven human herpes viruses. J. Med. Virol. 2008, 80:1042-1050.