Clinical validation of the lyra direct HSV 1+2/VZV assay for simultaneous detection and differentiation of three herpesviruses in Cutaneous and Mucocutaneous Lesions

Journal of Clinical Microbiology

Volumn 52, Issue 10, 2014, Pages 3799-3801

  Fan, Feinan ^a,b   Stiles, Jeffrey ^b   Mikhlina, Albina ^b   Lu, Xuedong ^a   Esther Babady, N ^b   Tang, Yi Wei ^b,c  

^a GUANGDONG MEDICAL UNIVERSITY   (China)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^c WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DIFFERENTIAL DIAGNOSIS; FEMALE; HERPES SIMPLEX VIRUS 1; HERPES SIMPLEX VIRUS 2; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; MEASUREMENT PRECISION; MUCOCUTANEOUS LESION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; POLYMERASE CHAIN REACTION SYSTEM; PREDICTIVE VALUE; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SKIN DEFECT; VALIDATION STUDY; VARICELLA ZOSTER VIRUS; VIRUS CULTURE; VIRUS DETECTION; AGED; CLASSIFICATION; GENETICS; HERPESVIRIDAE INFECTIONS; ISOLATION AND PURIFICATION; MIDDLE AGED; MOLECULAR DIAGNOSIS; MUCOSA; PROCEDURES; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; SKIN; VARICELLOVIRUS; VIROLOGY;

ADULT; AGED; FEMALE; HERPESVIRIDAE INFECTIONS; HERPESVIRUS 1, HUMAN; HERPESVIRUS 2, HUMAN; HUMANS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; MUCOUS MEMBRANE; MULTIPLEX POLYMERASE CHAIN REACTION; PROSPECTIVE STUDIES; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SKIN; VARICELLOVIRUS;

EID: 84907185205     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02098-14     Document Type: Article

References (17)

1. Tan HH, Goh CL. 2006. Viral infections affecting the skin in organ transplant recipients: epidemiology and current management strategies. Am. J. Clin. Dermatol. 7:13-29. http://dx.doi.org/10.2165/00128071-200607010-00003.
2. Kalman CM, Laskin OL. 1986. Herpes zoster and zosteriform herpes simplex virus infections in immunocompetent adults. Am. J. Med. 81: 775-778. http://dx.doi.org/10.1016/0002-9343(86)90343-8.
3. Frisch S, Guo AM. 2013. Diagnostic methods and management strategies of herpes simplex and herpes zoster infections. Clin. Geriatr. Med. 29: 501-526. http://dx.doi.org/10.1016/j.cger.2013.01.003.
4. Styczynski J, Reusser P, Einsele H, de la Camara R, Cordonnier C, Ward KN, Ljungman P, Engelhard D. 2009. Management of HSV, VZV and EBV infections in patients with hematological malignancies and after SCT: guidelines from the Second European Conference on Infections in Leukemia. Bone Marrow Transpl. 43:757-770. http://dx.doi.org/10.1038/bmt.2008.386.
5. Druce J, Catton M, Chibo D, Minerds K, Tyssen D, Kostecki R, Maskill B, Leong-Shaw W, Gerrard M, Birch C. 2002. Utility of a multiplex PCR assay for detecting herpesvirusDNAin clinical samples. J. Clin. Microbiol. 40:1728-1732. http://dx.doi.org/10.1128/JCM.40.5.1728-1732.2002.
6. Espy MJ, Uhl JR, Sloan LM, Buckwalter SP, Jones MF, Vetter EA, Yao JD, Wengenack NL, Rosenblatt JE, Cockerill FR, III, Smith TF. 2006. Real-time PCR in clinical microbiology: Applications for routine laboratory testing. Clin. Microbiol. Rev. 19:165-256. http://dx.doi.org/10.1128/CMR.19.1.165-256.2006.
7. Tan TY, Zou H, Ong DC, Ker KJ, Chio MT, Teo RY, Koh MJ. 2013. Development and clinical validation of a multiplex real-time PCR assay for herpes simplex and varicella zoster virus. Diagn. Mol. Pathol. 22:245-248. http://dx.doi.org/10.1097/PDM.0b013e3182914291.
8. Bennett S, Carman WF, Gunson RN. 2013. The development of a multiplex real-time PCR for the detection of herpes simplex virus 1 and 2, varizella [sic] zoster virus, adenovirus and Chlamydia trachomatis from eye swabs. J. Virol. Methods 189:143-147. http://dx.doi.org/10.1016/j.jviromet.2012.08.020.
9. Buelow DR, Bankowski MJ, Fofana D, Gu Z, Pounds S, Hayden RT. 2013. Comparison of two multiplexed PCR assays for the detection of HSV-1, HSV-2, and VZV with extracted and unextracted cutaneous and mucosal specimens. J. Clin. Virol. 58:84-88. http://dx.doi.org/10.1016/j.jcv.2013.05.008.
10. Fan F, Day S, Lu X, Tang YW. 2014. Laboratory diagnosis of herpes simplex virus and varicella-zoster virus infections. Future Virol., in press.
11. Podzorski RP. 2006. Evaluation of the Cepheid herpes simplex virus typing real-time PCR assay using dermal and genital specimens. Diagn. Microbiol. Infect. Dis. 56:173-177. http://dx.doi.org/10.1016/j.diagmicrobio.2006.03.011.
12. Dekeyser S, El Nady M, Diaz E, Cassagnou M, Descamps D. 2009. Diagnosis of herpetic esophagitis in the immunocompetent subject by PCR (Herpès Consensus Générique-Argène). Report of six cases. Pathol. Biol. (Paris) 57:101-106. (In French.) http://dx.doi.org/10.1016/j.patbio.2008.07.005.
13. Tang YW, Espy MJ, Persing DH, Smith TF. 1997. Molecular evidence and clinical significance of herpesvirus coinfection in the central nervous system. J. Clin. Microbiol. 35:2869-2872.
14. Sakai K, Wakasugi S, Muchemwa FC, Ihn H. 2008. Quick detection of herpes viruses from skin vesicles and exudates without nucleic acid extraction using multiplex PCR. Biosci. Trends 2:164-168.
15. Pandori MW, Lei J, Wong EH, Klausner J, Liska S. 2006. Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction. BMC Infect. Dis. 6:104. http://dx.doi.org/10.1186/1471-2334-6-104.
16. Binkhamis K, Al-Siyabi T, Heinstein C, Hatchette TF, LeBlanc JJ. 2014. Molecular detection of varicella zoster virus while keeping an eye on the budget. J. Virol. Methods 202:24-27.http://dx.doi.org/10.1016/j.jviromet.2014.02.009.
17. Mehta SK, Tyring SK, Cohrs RJ, Gilden D, Feiveson AH, Lechler KJ, Pierson DL. 2013. Rapid and sensitive detection of varicella zoster virus in saliva of patients with herpes zoster. J. Virol. Methods 193:128-130. http://dx.doi.org/10.1016/j.jviromet.2013.05.019.