Development and optimization of a real-time PCR assay for detection of herpes simplex and varicella-zoster viruses in skin and mucosal lesions by use of the BD Max open system

Journal of Clinical Microbiology

Volumn 52, Issue 12, 2014, Pages 4375-4376

  Cárdenas, Ana María ^a,b   Edelstein, Paul H ^a   Alby, Kevin ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b CHILDREN S HOSPITAL OF PHILADELPHIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

REAGENT; VIRUS DNA;

ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; HERPES SIMPLEX VIRUS; HERPES SIMPLEX VIRUS 1; HERPES SIMPLEX VIRUS 2; HUMAN; HUMAN TISSUE; LIMIT OF DETECTION; MOLECULAR PROBE; MUCOSAL DISEASE; NONHUMAN; POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION SYSTEM; PROCESS OPTIMIZATION; REAL TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDY; SKIN DEFECT; VARICELLA ZOSTER VIRUS; VIRUS DETECTION; VIRUS LOAD; EVALUATION STUDY; GENETICS; HERPES SIMPLEX; HERPES ZOSTER; ISOLATION AND PURIFICATION; MOLECULAR DIAGNOSIS; PROCEDURES; SIMPLEXVIRUS; SKIN; VIROLOGY;

HERPES SIMPLEX; HERPES ZOSTER; HERPESVIRUS 3, HUMAN; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; REAL-TIME POLYMERASE CHAIN REACTION; SIMPLEXVIRUS; SKIN; VIROLOGY;

EID: 84910117068     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02237-14     Document Type: Article

References (9)

1. Alby K, Popowitch EB, Miller MB. 2013. Comparative evaluation of the Nanosphere Verigene RV+ assay and the Simplexa Flu A/B & RSV kit for detection of influenza and respiratory syncytial viruses. J. Clin. Microbiol. 51:352-353. http://dx.doi.org/10.1128/JCM.02504-12.
2. Church D, Gregson D, Lloyd T, Klein M, Beckthold B, Laupland K, Gill MJ. 2011. Comparison of the RealTime HIV-1, COBAS TaqMan v1.0, Easy Q. v1.2, and Versant v3.0 assays for the determination of HIV-1 viral loads in a cohort of Canadian patients with diverse HIV subtype infections. J. Clin. Microbiol. 49:118-124. http://dx.doi.org/10.1128/JCM.00685-10.
3. Hirsch HH, Lautenschlager I, Pinsky BA, Cardenoso L, Aslam S, Cobb B, Vilchez RA, Valsamakis A. 2013. An international multicenter performance analysis of cytomegalovirus load tests. Clin. Infect. Dis. 56:367-373. http://dx.doi.org/10.1093/cid/cis900.
4. Popowitch EB, O'Neill SS, Miller MB. 2013. Comparison of the Biofire FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1, and Luminex xTAG RVP fast multiplex assays for detection of respiratory viruses. J. Clin. Microbiol. 51:1528-1533. http://dx.doi.org/10.1128/JCM.03368-12.
5. Dalpke AH, Hofko M, Zimmermann S. 2013. Development and evaluation of a real-time PCR assay for detection of Pneumocystis jirovecii on the fully automated BD MAX platform. J. Clin. Microbiol. 51:2337-2343. http://dx.doi.org/10.1128/JCM.00616-13.
6. Hofko M, Mischnik Kaase AM, Zimmermann S, Dalpke AH. 2014. Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system. J. Clin. Microbiol. 52:1701-1704. http://dx.doi.org/10.1128/JCM.00373-14.
7. Burd EM. 2010. Validation of laboratory-developed molecular assays for infectious diseases. Clin. Microbiol. Rev. 23:550-576. http://dx.doi.org/10.1128/CMR.00074-09.
8. Pevenstein SR, Williams RK, McChesney D, Mont EK, Smialek JE, Straus SE. 1999. Quantitation of latent varicella-zoster virus and herpes simplex virus genomes in human trigeminal ganglia. J. Virol. 73:10514-10518.
9. Filen F, Strand A, Allard A, Blomberg J, Herrmann B. 2004. Duplex real-time polymerase chain reaction assay for the detection and quantification of herpes simplex virus type 1 and herpes simplex virus type 2 in genital and cutaneous lesions. Sex. Transm. Dis. 31:331-336. http://dx.doi.org/10.1097/00007435-200406000-00002.