Novel applications of magnetic cell sorting to analyze cell-type specific gene and protein expression in the central nervous system

PLoS ONE

Volumn 11, Issue 2, 2016, Pages

  Holt, Leanne Melissa ^a   Olsen, Michelle Lynne ^a,b  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD11B ANTIGEN; EXCITATORY AMINO ACID TRANSPORTER 1; EXCITATORY AMINO ACID TRANSPORTER 2; MICROTUBULE ASSOCIATED PROTEIN 2; NEURON SPECIFIC NUCLEAR PROTEIN; RNA; TOLL LIKE RECEPTOR 4; BIOLOGICAL MARKER; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; NERVE PROTEIN; SLC1A2 PROTEIN, RAT;

ADULT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ASTROCYTE; CELL ISOLATION; CELL POPULATION; CELL SELECTION; CELLULAR DISTRIBUTION; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; EXTRACTION; GENE; GENE EXPRESSION; GFAP GENE; HEXB GENE; ITGAM GENE; JUVENILE ANIMAL; MAGNETIC CELL SORTING; MAP2 GENE; MBP GENE; MICROGLIA; MOUSE; NEFM GENE; NONHUMAN; OLIGODENDROGLIA; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN EXTRACTION; RAT; RBFOX3 GENE; RNA SEQUENCE; SLC1A2 GENE; SLC1A3 GENE; TLR4 GENE; ANIMAL; BRAIN CORTEX; C57BL MOUSE; CYTOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; IMMUNOLOGY; IMMUNOMAGNETIC SEPARATION; METABOLISM; NERVE CELL; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; ASTROCYTES; BIOMARKERS; CEREBRAL CORTEX; EXCITATORY AMINO ACID TRANSPORTER 2; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; IMMUNOMAGNETIC SEPARATION; MICE; MICE, INBRED C57BL; MICROGLIA; NERVE TISSUE PROTEINS; NEURONS; RATS; REAL-TIME POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 84960381276     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0150290     Document Type: Article

References (44)

1. Maezawa I, Swanberg S, Harvey D., LaSalle JM, Jin LW. Rett Syndrome Astrocytes Are Abnormaland Spread MeCP2 Deficiency through Gap Junctions. Journal of Neuroscience. 2009; 29(16):5051-61. doi: 10.1523/JNEUROSCI.0324-09.2009 PMID: 19386901
2. Derecki NC, CronkJ C, Lu Z, Xu E, Abbott SB, Guyenet PG, et al. Wild-type microglia arrest pathology in a mouse model of Rett syndrome. Nature. 2012; 484(7392):105-9. doi: 10.1038/nature10907 PMID: 22425995
3. Gilliam D, O'Brien DP, Coates JR, Johnson G.S., Johnson GC, Mhlanga-Mutangadura T, et al. A Homozygous KCNJ10 Mutation in Jack Russell Terriers and Related Breeds with Spinocerebellar Ataxia with Myokymia, Seizures, or Both. J Vet Intern Med. 2014.
4. Lovatt D, Sonnewald U, Waagepetersen H.S., Schousboe A., He W, Lin JH, et al The transcriptome and metabolic gene signature of protoplasmic astrocytes in the adult murine cortex. J Neuro sci. 2007; 27 (45):12255-66.
5. Chadi G, Maximino JR, de Oliveira GP. The importance of molecular histology to study glial influence on neurodegenerative disorders. Focus on recent developed single cell laser microdissection. J Mol Histol. 2009; 40(4):241-50.
6. Standaert DG. Applications of laser capture microdissection in the study of neurodegenerative disease. Arch Neurol. 2005; 62(2):203-5.
7. Feldmann M, Pathipati P, Sheldon R., Jiang X, Ferriero D. Isolating astrocytes and neurons sequentially from postnatal murine brains with a magnetic cell separation technique. Journal of Biological Methods. 2014; 1(2).
8. Ballas N, Lioy DT, Grunseich C, Mandel G. Non-cell autonomous influence of MeCP2-deficient glia on neuronal dendritic morphology. Nat Neurosci. 2009; 12(3):311-7. doi: 10.1038/nn.2275 PMID: 19234456
9. Foo LC. Purification of rat and mouse astrocytes by immunopanning. Cold Spring HarbProtoc. 2013; 2013(5):421-32.
10. Dugas JC, Mandemakers W, Rogers M., Ibrahim A, Daneman R, Barres BA A novel purification method for CNS projection neurons leads to the identification of brain vascular cells as a source of trophic support for corticospinal motor neurons. J Neuro sci. 2008; 28(33):8294-305.
11. Emery B, Dugas JC Purification of oligodendrocyte lineage cells from mouse cortices by immunopanning. Cold Spring Harb Protoc. 2013; 2013(9):854-68.
12. McCullumsmith RE, Meador-Woodruff JH. Novel approaches to the study of postmortem brain in psychiatric illness: old limitations and new challenges. BiolPsychiatry. 2011; 69(2):127-33.
13. Cahoy JD, Emery B, Kaushal A., Foo LC, Zamanian JL, Christopherson KS, et al A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function. J Neurosci. 2008; 28(1):264-78. doi: 10.1523/JNEUROSCI.4178-07.2008 PMID: 18171944
14. Zhang Y, Chen K, Sloan S.A., Bennett ML, Scholze A R, O'Keeffe S, et al. An RNA-sequencing transcriptome and splicing database of glia, neurons, and vascular cells of the cerebral cortex. J Neuro sci. 2014; 34(36):11929-47.
15. Guez-Barber D., Fanous S, Harvey B.K., Zhang Y., Lehrmann E, Becker KG, et al FACS purification of immunolabeled cell types from adult rat brain. J Neuro sci Methods. 2012; 203(1):10-8.
16. Lobo MK, Karsten SL, Gray M, Geschwind D.H., Yang XW. FACS-array profiling of striatal projection neuron subtypes in juvenile and adult mouse brains. Nat Neuro sci. 2006; 9(3):443-52.
17. Smith SM, Kimyon RS, Watters JJ Cell-type-specific Jumonji histone demethylase gene expression in the healthy rat CNS: detection by a novel flow cytometry method. ASNNeuro. 2014; 6(3):193-207.
18. Marek R, Caruso M, Rostami A., Grinspan JB, Das SJ. Magnetic cell sorting: a fast and effective method of concurrent isolation of high purity viable astrocytes and microglia from neonatal mouse brain tissue. JNeurosciMethods. 2008; 175(1):108-18.
19. Miltenyi S, Muller W, Weichel W., Radbruch A. High gradient magnetic cell separation with MACS. Cytometry. 1990; 11(2):231-8. PMID: 1690625
20. Dincman T A, Beare JE, Ohri S.S., Whittemore SR. Isolation of cortical mouse oligodendrocyte precursor cells. J Neuro sci Methods. 2012; 209(1):219-26.
21. Jungblut M, Tiveron MC, Barral S, Abrahamsen B., Knobel S, Pennartz S, et al Isolation and characterization of living primary astroglial cells using the new GLAST-specific monoclonal antibody ACSA-1. Glia. 2012; 60(6):894-907. doi: 10.1002/glia.22322 PMID: 22374709
22. Yang Y, Vidensky S, Jin L., Jie C, Lorenzini I, Frankl M., et al. Molecular comparison of GLT1+and ALDH1L1+ astrocytes in vivo in astroglial reporter mice. Glia. 2011; 59(2):200-7. doi: 10.1002/glia. 21089 PMID: 21046559
23. Nwaobi SE, Lin E, Peramsetty S.R., Olsen ML. DNA methylation functions as a critical regulator of Kir4.1 expression during CNS development. Glia. 2014; 62(3):411-27. doi: 10.1002/glia.22613 PMID: 24415225
24. Nwaobi S, Lyn E, Permasetty S., Olsen M. DNA methylation is a criticial regulatorof Kir4.1 during development. 2013.
25. Matsutani S, Yamamoto N. Neuronal regulation of astrocyte morphology in vitro is mediated by GABAergic signaling. Glia. 1997; 20(1):1-9. PMID: 9145300
26. van Landeghem F.K., Stover JF, Bechmann I, Bruck W., Unterberg A, Buhrer C, et al Early expression of glutamate transporter proteins in ramified microglia after controlled cortical impact injury in the rat. Glia. 2001; 35(3):167-79. PMID: 11494408
27. Persson M, Brantefjord M, Hansson E., Ronnback L. Lipopolysaccharide increases microglial GLT-1 expression and glutamate uptake capacity in vitro by a mechanism dependent on TNF-alpha. Glia. 2005; 51(2):111-20. PMID: 15789431
28. Itakura E, Odaira K, Yokoyama K., Osuna M, Hara T, Inoue K. Generation of transgenic rats expressing green fluorescent protein in S-100beta-producing pituitary folliculo-stellate cells and brain astrocytes. Endocrinology. 2007; 148(4):1518-23. PMID: 17234709
29. Ludwin SK, Kosek JC, Eng LF The topographical distribution of S-100 and GFA proteins in the adult rat brain: an immunohistochemical study using horseradish peroxidase-labelled antibodies. J Comp Neurol. 1976; 165(2):197-207. PMID: 1107363
30. Ferraiuolo L, Heath PR, Holden H, Kasher P., Kirby J, Shaw PJ. Microarray analysis of the cellular pathways involved in the adaptation to and progression of motor neuron injury in the SOD1 G93A mouse model of familial ALS. J Neurosci. 2007; 27(34):9201-19. PMID: 17715356
31. Sugino K, HempelC M, Okaty B.W., Arnson HA, Kato S, Dani VS, et al Cell-Type-Specific Repression by Methyl-CpG-Binding Protein 2 Is Biased toward Long Genes. JNeurosci. 2014; 34(38):12877-83.
32. Dong W, Vuletic S, Albers JJ Differential effects of simvastatin and pravastatin on expression of Alzheimer's disease-related genes in human astrocytes and neuronal cells. Journal of lipid research. 2009; 50(10):2095-102. doi: 10.1194/jlr.M900236-JLR200 PMID: 19461118
33. Garg SK, Lioy DT, Cheval H, McGann J.C., Bissonnette JM, Murtha MJ, et al. Systemic Delivery of MeCP2 Rescues Behavioral and Cellular Deficits in Female Mouse Models of Rett Syndrome. JNeurosci. 2013; 33a(34):13612-20.
34. Guy J, Gan J, Selfridge J., Cobb S, Bird A. Reversal of Neurological Defects in a Mouse Model of Rett Syndrome. Science. 2007; 315(5815):1143-7. PMID: 17289941
35. Lioy DT, GargS K, Monaghan C.E., Raber J., Foust KD, Kaspar BK, et al. A role forglia in the progression of Rett's syndrome. Nature. 2011; 475(7357):497-500. doi: 10.1038/nature10214 PMID: 21716289
36. Luikenhuis S, Giacometti E, Beard C.F., Jaenisch R. Expression of MeCP2 in postmitotic neurons rescues Rett syndrome in mice. Proc Natl Acad Sci USA. 2004; 101(16):6033-8.
37. Losciuto S, Dorban G, Gabel S., Gustin A, Hoenen C, Grandbarbe L., et al. An efficient method to limit microglia-dependent effects in astroglial cultures. J Neurosci Methods. 2012; 207(1):59-71. doi: 10. 1016/j.jneumeth.2012.03.010 PMID: 22483759
38. Harms AS, Tansey MG Isolation of murine postnatal brain microgliafor phenotypic characterization using magnetic cell separation technology. Methods Mol Biol. 2013; 1041):33-9. doi: 10.1007/978-1-62703-520-05 PMID: 23813367
39. Ohtsuki S, Yamaguchi H, Katsukura Y., Asashima T, Terasaki T. mRNA expression levels of tight junction protein genes in mouse brain capillary endothelial cells highly purified by magnetic cell sorting. J Neurochem. 2008; 104(1):147-54. PMID: 17971126
40. Nikodemova M, Watters JJ Efficient isolation of live microglia with preserved phenotypes from adult mouse brain. Journal of neuroinflammation. 2012; 9:147. doi: 10.1186/1742-2094-9-147 PMID: 22742584
41. Shirao T, Sekino Y. Clustering and anchoring mechanisms of molecular constituents of postsynaptic scaffolds in dendritic spines. Neuroscience research. 2001; 40(1):1-7. PMID: 11311400
42. Jacob AL, Weinberg RJ The organization of AMPA receptor subunits at the postsynaptic membrane. Hippocampus. 2015; 25(7):798-812. doi: 10.1002/hipo.22404 PMID: 25524891
43. Nagelhus EA, Mathiisen TM, Ottersen OP Aquaporin-4 in the central nervous system: cellular and subcellular distribution and coexpression with KIR4.1. Neuroscience. 2004; 129(4):905-13. PMID: 15561407
44. NagyJ I, Rash JE Astrocyte and oligodendrocyte connexins of the glial syncytium in relation to astrocyte anatomical domains and spatial buffering. Cell Commun Adhes. 2003; 10(4-6:401-6. PMID: 14681048