Triton Hodge Test: Improved Protocol for Modified Hodge Test for Enhanced Detection of NDM and Other Carbapenemase Producers

Journal of Clinical Microbiology

Volumn 54, Issue 3, 2016, Pages 640-649

  Pasteran, Fernando ^a   Gonzalez, Lisandro J ^b   Albornoz, Ezequiel ^a   Bahr, Guillermo ^b   Vila, Alejandro J ^b   Corso, Alejandra ^a  

^a INSTITUTO NACIONAL DE ENFERMEDADES INFECCIOSAS   (Argentina)

^b UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

CARBAPENEMASE; ERTAPENEM; IMIPENEM; MEROPENEM; METALLO BETA LACTAMASE; NEW DELHI METALLO BETA LACTAMASE; NONIONIC SURFACTANT; TRITON X 100; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; BACTERIAL PROTEIN; BETA LACTAM; BETA LACTAMASE; DETERGENT; OCTOXINOL; THIENAMYCIN DERIVATIVE;

ACINETOBACTER BAUMANNII; ACINETOBACTER PITTII; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL OUTER MEMBRANE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BROTH DILUTION; CITROBACTER; CITROBACTER AMALONATICUS; CITROBACTER BRAAKII; CITROBACTER FREUNDII; CONTROLLED STUDY; ENTEROBACTER AEROGENES; ENTEROBACTER CLOACAE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INACTIVATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; GRAM NEGATIVE BACTERIUM; KLEBSIELLA PNEUMONIAE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MINIMUM INHIBITORY CONCENTRATION; MORGANELLA MORGANII; NONHUMAN; PRIORITY JOURNAL; PROVIDENCIA RETTGERI; PROVIDENCIA STUARTII; SENSITIVITY AND SPECIFICITY; SERRATIA MARCESCENS; DRUG EFFECTS; ENZYMOLOGY; HUMAN; METABOLISM; MICROBIAL SENSITIVITY TEST; PROCEDURES;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BETA-LACTAMASES; BETA-LACTAMS; DETERGENTS; GRAM-NEGATIVE BACTERIA; HUMANS; MICROBIAL SENSITIVITY TESTS; OCTOXYNOL; SENSITIVITY AND SPECIFICITY; THIENAMYCINS;

EID: 84959526446     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01298-15     Document Type: Article

References (29)

1. Bush K, Jacoby G. 2010. Updated functional classification of-lactamases. Antimicrob Agents Chemother 54:969-976. http://dx.doi.org/10.1128/AAC.01009-09.
2. Clinical and Laboratory Standards Institute. 2015. Performance standards for antimicrobial susceptibility testing; 25th informational supplement. CLSI M100-S25. Clinical and Laboratory Standards Institute, Wayne, PA.
3. Doyle D, Peirano G, Lascols C, Lloyd T, Church DL, Pitout JD. 2012. Laboratory detection of Enterobacteriaceae that produce carbapenemases. J Clin Microbiol 50:3877-3880. http://dx.doi.org/10.1128/JCM.02117-12.
4. Girlich D, Poirel L, Nordmann P. 2012. Value of the modified Hodge test for detection of emerging carbapenemases in Enterobacteriaceae. J Clin Microbiol 50:477-479. http://dx.doi.org/10.1128/JCM.05247-11.
5. Castanheira M, Deshpande LM, Mathai D, Bell JM, Jones RN, Mendes RE. 2011. Early dissemination of NDM-1-and OXA-181-producing Enterobacteriaceae in Indian hospitals: Report from the SENTRY Antimicrobial Surveillance Program, 2006-2007. Antimicrob Agents Chemother 55:1274-1278. http://dx.doi.org/10.1128/AAC.01497-10.
6. Saito R, Koyano S, Dorin M, Higurashi Y, Misawa Y, Nagano N, Kaneko T, Moriya K. 2015. Evaluation of a simple phenotypic method for the detection of carbapenemase-producing Enterobacteriaceae. J Microbiol Methods 108:45-48. http://dx.doi.org/10.1016/j.mimet.2014.11.008.
7. Bonnin RA, Naas T, Poirel L, Nordmann P. 2012. Phenotypic, biochemical, and molecular techniques for detection of metallo-lactamaseNDM in Acinetobacter baumannii. J Clin Microbiol 50:1419-1421. http://dx.doi.org/10.1128/JCM.06276-11.
8. Meini M, Tomatis P, Weinreich D, Vila AJ. 2015. Quantitative description of a protein fitness landscape based on molecular features. Mol Biol Evol 32:1774-1787. http://dx.doi.org/10.1093/molbev/msv059.
9. Kim HK, Park JS, Sung H, Kim MN. 2015. Further modification of the modified Hodge test for detecting metallo-lactamase-producing carbapenem-resistant Enterobacteriaceae. Ann Lab Med 35:298-305. http://dx.doi.org/10.3343/alm.2015.35.3.298.
10. King D, Strynadka N. 2011. Crystal structure of New Delhi metallo-lactamase reveals molecular basis for antibiotic resistance. Protein Sci 20: 1484-1491. http://dx.doi.org/10.1002/pro.697.
11. Gonzalez L, Bahr G, Bonomo RA, Vila A. 2014. Membrane anchoring of carbapenemase NDM-1 favors protein stability and resistance transfer. Abstr 54th Intersci Conf Antimicrob Agents Chemother, abstr C-162c.
12. Moran-Barrio J, Lisa M, Vila A. 2012. In vivo impact of Met221 substitution in GOB metallo-lactamase. Antimicrob Agents Chemother 56: 1769-1773. http://dx.doi.org/10.1128/AAC.05418-11.
13. Choi K, Kumar A, Schweizer H. 2006. A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: Application for DNA fragment transfer between chromosomes and plasmid transformation. J Microbiol Methods 64:391-397. http://dx.doi.org/10.1016/j.mimet .2005.06.001.
14. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing. J Clin Microbiol 33:2233-2239.
15. Almuzara M, Barberis C, Traglia G, Famiglietti A, Ramirez MS, Vay C. 2015. Evaluation of matrix-assisted laser desorption ionization-time-offlight mass spectrometry for species identification of nonfermenting Gram-negative bacilli. J Microbiol Methods 112:24-27. http://dx.doi.org/10.1016/j.mimet.2015.03.004.
16. Pasteran F, Veliz O, Ceriana P, Lucero C, Rapoport M, Albornoz E, Gomez S, Corso A. 2015. Evaluation of the Blue-Carba test for rapid detection of carbapenemases in Gram-negative bacilli. J Clin Microbiol 53:1996-1998. http://dx.doi.org/10.1128/JCM.03026-14.
17. Melano R, Corso A, Petroni A, Centron D, Orman B, Pereyra A, Moreno N, Galas M. 2003. Multiple antibiotic-resistance mechanisms including a novel combination of extended-spectrum-lactamases in a Klebsiella pneumoniae clinical strain isolated in Argentina. J Antimicrob Chemother 52:36-42. http://dx.doi.org/10.1093/jac/dkg281.
18. Martínez-Martínez L, Hernández-Allés S, Abertí S, Tomás J, Benedi V, Jacoby G. 1996. In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expandedspectrum cephalosporins. Antimicrob Agents Chemother 40:342-348.
19. Hamzehpour M, Pecher J, Plesiat P, Kohler T. 1995. OprK and OprM define two genetically distinct multidrug efflux systems in Pseudomonas aeruginosa. Antimicrob Agents Chemother 39:2392-2396. http://dx.doi.org/10.1128/AAC.39.11.2392.
20. Pasteran F, Mendez T, Rapoport M, Guerriero L, Corso A. 2010. Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class A carbapenemase in species of Enterobacteriaceae by incorporating boronic acid. J Clin Microbiol 48:1323-1332. http://dx.doi.org/10.1128/JCM.01771-09.
21. Ribeiro VB, Linhares AR, Zavascki AP, Barth AL. 2014. Performance of quantification of modified Hodge test: An evaluation with Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae isolates. Biomed Res Int http://dx.doi.org/10.1155/2014/139305.
22. Carvalhaes CG, Picão RC, Nicoletti AG, Xavier DE, Gales AC. 2010. Cloverleaf test (modified Hodge test) for detecting carbapenemase production in Klebsiella pneumoniae: Be aware of false positive results. J Antimicrob Chemother 65:249-251. http://dx.doi.org/10.1093/jac/dkp431.
23. Dortet L, Bre'chard L, Poirel L, Nordmann P. 2014. Impact of the isolation medium for detection of carbapenemase-producing Enterobacteriaceae using an updated version of the Carba NP. J Med Microbiol 63:772-776. http://dx.doi.org/10.1099/jmm.0.071340-0.
24. Cooper GL, Louie A, Baltch AL, Chu RC, Smith RP, Ritz WJ, Michelsen P. 1993. Influence of zinc on Pseudomonas aeruginosa susceptibilities to imipenem. J Clin Microbiol 31:2366-2370.
25. Schnaitman CA. 1971. Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100. J Bacteriol 108:545-552.
26. Shang ES, Summers TA, Haake DA. 1996. Molecular cloning and sequence analysis of the gene encoding LipL41, a surface-exposed lipoprotein of pathogenic Leptospira species. Infect Immun 64:2322-2330.
27. Crago AM, Koronakis V. 1998. Salmonella InvG forms a ring-like multimer that requires the InvH lipoprotein for outer membrane localization. Mol Microbiol 30:47-56. http://dx.doi.org/10.1046/j.1365-2958.1998 .01036.x.
28. Jones N. 1999. Surfactants in membrane solubilisation. Int J Pharm 177: 137-159. http://dx.doi.org/10.1016/S0378-5173(98)00345-7.
29. Pasteran F, Veliz O, Rapoport M, Guerriero L, Corso A. 2011. Sensitive and specific modified Hodge test for KPC and metallo-lactamase detection in Pseudomonas aeruginosa by use of a novel indicator strain, Klebsiella pneumoniae ATCC 700603. J Clin Microbiol 49:4301-4303. http://dx.doi.org/10.1128/JCM.05602-11.