Cloverleaf test (modified Hodge test) for detecting carbapenemase production in Klebsiella pneumoniae: be aware of false positive results

Journal of Antimicrobial Chemotherapy

Volumn 65, Issue 2, 2009, Pages 249-251

  Carvalhaes, Cecilia G ^a   Picão, Renata C ^a   Nicoletti, Adriana G ^a   Xavier, Danilo E ^a   Gales, Ana C ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Carbapenem resistance; CTX M; KPC; OmpK35; OmpK36

Indexed keywords

CARBAPENEM; CARBAPENEMASE; CEFEPIME; CEFTAZIDIME; ERTAPENEM; IMIPENEM; MEROPENEM; OUTER MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN K35; OUTER MEMBRANE PROTEIN K36; PORIN; UNCLASSIFIED DRUG;

AGAR DILUTION; AMPLICON; ANALYTIC METHOD; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM ISOLATE; CLOVERLEAF TEST; DRUG HYDROLYSIS; ENZYME SYNTHESIS; FALSE POSITIVE RESULT; GENE EXPRESSION; GENE IDENTIFICATION; GENE SEQUENCE; GENOTYPE; INOCULATION; KLEBSIELLA PNEUMONIAE; MOLECULAR TYPING; NONHUMAN; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; SPECTROPHOTOMETRY;

EID: 77950211733     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkp431     Document Type: Article

References (11)

1. Andrade S, Sader HS, Barth A et al. Antimicrobial susceptibility of Gram-negative bacilli isolated in Brazilian hospitals participatingin the SENTRY Program (2003-2008). Braz J Infect Dis 2008; 12: 3-9.
2. Martinez-Martinez L, Pascual A, Hernandez-Alles S et al. Roles of β-lactamases and porins in activities of carbapenems and cephalosporins against Klebsiella pneumoniae. Antimicrob Agents Chemother 1999; 43: 1669-73.
3. Doumith M, Ellington MJ, Livermore DM et al. Molecular mechanisms disrupting porin expression in ertapenem-resistant Klebsiella and Enterobacter spp. clinical isolates from the UK. J Antimicrob Chemother 2009; 63: 659-67.
4. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: Nineteenth Informational Supplement M100-S19. CLSI, Wayne, PA, USA, 2009.
5. Orstavik I, Odegaard K. A simple test for penicillinase production in Staphylococcus aureus. Acta Pathol Microbiol Scand B Microbiol Immunol 1971; 79: 855-6.
6. Picão RC, Poirel L, Gales AC et al. Diversity of β-lactamases produced by ceftazidime-resistant Pseudomonas aeruginosa isolates causing bloodstream infections in Brazil. Antimicrob Agents Chemother 2009; 53: 3908-13.
7. Woodford N, Ellington MJ, Coelho JM et al. Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int J Antimicrob Agents 2006; 27: 351-3.
8. Hernández-Allés S, Albertí S, Alvarez D et al. Porin expression in clinical isolates of Klebsiella pneumoniae. Microbiology 1999; 145: 673-9.
9. Tenover FC, Arbeit RD, Goering RV et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33: 2233-9.
10. Girlich D, Poirel L, Nordmann P. Do CTX-M β-lactamases hydrolyse ertapenem? J Antimicrob Chemother 2008; 62: 1155-6.
11. Queenan AM, Foleno B, Gownley C et al. Effects of inoculum and β-lactamase activity in AmpCand extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae clinical isolates tested by using NCCLS ESBL methodology. J Clin Microbiol 2004; 42: 269-75.