Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2

Nature Protocols

Volumn 11, Issue 3, 2016, Pages 456-475

  Hung, Victoria ^a   Udeshi, Namrata D ^b   Lam, Stephanie S ^a   Loh, Ken H ^a   Cox, Kurt J ^a   Pedram, Kayvon ^a   Carr, Steven A ^b   Ting, Alice Y ^a,b  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b BROAD INSTITUTE OF MIT AND HARVARD   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; APEX2 PROTEIN; BIOTIN; BIOTIN PHENOXYL RADICAL; BIOTINYLATED PROTEIN; CELL PROTEIN; HYBRID PROTEIN; PEROXIDASE; PHENOL; PROTEIN DERIVATIVE; RADICAL; REAGENT; STREPTAVIDIN; UNCLASSIFIED DRUG; APEX2 PROTEIN, HUMAN; DNA (APURINIC OR APYRIMIDINIC SITE) LYASE; HYDROGEN PEROXIDE; PROTEOME;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIVISION; CELL MEMBRANE; CELLS; CONTROLLED STUDY; COVALENT BOND; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; INTRACELLULAR SPACE; ISOTOPE LABELING; LABORATORY DEVICE; LIVING CELL; MAMMAL CELL; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; SPATIALLY RESOLVED PROTEOMIC MAPPING; BIOTINYLATION; GENETICS; HEK293 CELL LINE; METABOLISM; PROCEDURES; PROTEIN ENGINEERING;

BIOTIN; BIOTINYLATION; DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE; HEK293 CELLS; HUMANS; HYDROGEN PEROXIDE; ISOTOPE LABELING; MASS SPECTROMETRY; PROTEIN ENGINEERING; PROTEOME; PROTEOMICS;

EID: 84959450407     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.018     Document Type: Article

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