Novel bilobe components in Trypanosoma brucei identified using proximity-dependent biotinylation

Eukaryotic Cell

Volumn 12, Issue 2, 2013, Pages 356-367

  Morriswood, Brooke ^a   Havlicek, Katharina ^a   Demmel, Lars ^a   Yavuz, Sevil ^a   Sealey Cardona, Marco ^a   Vidilaseris, Keni ^a   Anrather, Dorothea ^b   Kostan, Julius ^b   Djinović Carugo, Kristina ^b,c   Roux, Kyle J ^d   Warren, Graham ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b UNIVERSITY OF VIENNA   (Austria)

^c UNIVERSITY OF LJUBLJANA   (Slovenia)

^d SANFORD RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOSKELETON PROTEIN; PROTOZOAL PROTEIN;

ARTICLE; BIOTINYLATION; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN TRANSPORT; TRYPANOSOMA BRUCEI;

BIOTINYLATION; CYTOSKELETAL PROTEINS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN TRANSPORT; PROTOZOAN PROTEINS; TRYPANOSOMA BRUCEI BRUCEI;

EID: 84873149805     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00326-12     Document Type: Article

References (30)

1. Gull K. 1999. The cytoskeleton of trypanosomatid parasites. Annu. Rev. Microbiol. 53:629-655. doi:10.1146/annurev.micro.53.1.629.
2. Robinson DR, Sherwin T, Ploubidou A, Byard EH, Gull K. 1995. Microtubule polarity and dynamics in the control of organelle positioning, segregation, and cytokinesis in the trypanosome cell cycle. J. Cell Biol. 128:1163-1172.
3. Lacomble S, Vaughan S, Gadelha C, Morphew MK, Shaw MK, McIntosh JR, Gull K. 2009. Three-dimensional cellular architecture of the flagellar pocket and associated cytoskeleton in trypanosomes revealed by electron microscope tomography. J. Cell Sci. 122:1081-1090. doi:10.1242/jcs.045740.
4. Engstler M, Thilo L, Weise F, Grunfelder CG, Schwarz H, Boshart M, Overath P. 2004. Kinetics of endocytosis and recycling of the GPIanchored variant surface glycoprotein in Trypanosoma brucei. J. Cell Sci. 117:1105-1115. doi:10.1242/jcs.00938.
5. Lacomble S, Vaughan S, Gadelha C, Morphew MK, Shaw MK, McIntosh JR, Gull K. 2010. Basal body movements orchestrate membrane organelle division and cell morphogenesis in Trypanosoma brucei. J. Cell Sci. 123:2884-2891. doi:10.1242/jcs.074161.
6. Gull K. 2003. Host-parasite interactions and trypanosome morphogenesis: a flagellar pocketful of goodies. Curr. Opin. Microbiol. 6:365-370.
7. Henley GL, Lee CM, Takeuchi A. 1978. Electron microscopy observations on Trypanosoma brucei: freeze-cleaving and thin-sectioning study of the apical part of the flagellar pocket. Z Parasitenkd. 55:181-187.
8. Sherwin T, Gull K. 1989. The cell division cycle of Trypanosoma brucei brucei: timing of event markers and cytoskeletal modulations. Philos. Trans. R. Soc. Lond. B Biol. Sci. 323:573-588.
9. Vickerman K. 1969. On the surface coat and flagellar adhesion in trypanosomes. J. Cell Sci. 5:163-193.
10. Broadhead R, Dawe HR, Farr H, Griffiths S, Hart SR, Portman N, Shaw MK, Ginger ML, Gaskell SJ, McKean PG, Gull K. 2006. Flagellar motility is required for the viability of the bloodstream trypanosome. Nature 440: 224-227. doi:10.1038/nature04541.
11. Ralston KS, Kisalu NK, Hill KL. 2011. Structure-function analysis of dynein light chain 1 identifies viable motility mutants in bloodstreamform Trypanosoma brucei. Eukaryot. Cell 10:884-894. doi:10.1128/EC.00298-10.
12. Zhou Q, Liu B, Sun Y, He CY. 2011. A coiled-coil-and C2-domaincontaining protein is required for FAZ assembly and cell morphology in Trypanosoma brucei. J. Cell Sci. 124:3848-3858. doi:10.1242/jcs.087676.
13. May SF, Peacock L, Almeida Costa CI, Gibson WC, Tetley L, Robinson DR, Hammarton TC. 2012. The Trypanosoma brucei AIR9-like protein is cytoskeleton-associated and is required for nucleus positioning and accurate cleavage furrow placement. Mol. Microbiol. 84:77-92. doi:10.1111/j.1365-2958.2012.08008.x.
14. He CY, Pypaert M, Warren G. 2005. Golgi duplication in Trypanosoma brucei requires Centrin2. Science 310:1196-1198. doi:10.1126/science.1119969.
15. Morriswood B, He CY, Sealey-Cardona M, Yelinek J, Pypaert M, Warren G. 2009. The bilobe structure of Trypanosoma brucei contains a MORN-repeat protein. Mol. Biochem. Parasitol. 167:95-103. doi:10.1016/j.molbiopara.2009.05.001.
16. Zhou Q, Gheiratmand L, Chen Y, Lim TK, Zhang J, Li S, Xia N, Liu B, Lin Q, He CY. 2010. A comparative proteomic analysis reveals a new bi-lobe protein required for bi-lobe duplication and cell division in Trypanosoma brucei. PLoS One 5:e9660. doi:10.1371/journal.pone.0009660.
17. Shi J, Franklin JB, Yelinek JT, Ebersberger I, Warren G, He CY. 2008. Centrin4 coordinates cell and nuclear division in T. brucei. J. Cell Sci. 121:3062-3070. doi:10.1242/jcs.030643.
18. Wang M, Gheiratmand L, He CY. 2012. An interplay between Centrin2 and Centrin4 on the bi-lobed structure in Trypanosoma brucei. Mol. Microbiol. 83:1153-1161. doi:10.1111/j.1365-2958.2012.07998.x.
19. Esson HJ, Morriswood B, Yavuz S, Vidilaseris K, Dong G, Warren G. 2012. Morphology of the trypanosome bilobe, a novel cytoskeletal structure. Eukaryot. Cell 11:761-772. doi:10.1128/EC.05287-11.
20. Roux KJ, Kim DI, Raida M, Burke B. 2012. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J. Cell Biol. 196:801-810. doi:10.1083/jcb.201112098.
21. Lane MD, Young DL, Lynen F. 1964. The enzymatic synthesis of holotranscarboxylase from apotranscarboxylase and (+)-biotin. I. Purification of the apoenzyme and synthetase; characteristics of the reaction. J. Biol. Chem. 239:2858-2864.
22. Kwon K, Beckett D. 2000. Function of a conserved sequence motif in biotin holoenzyme synthetases. Protein Sci. 9:1530-1539. doi:10.1110/ps.9.8.1530.
23. Wirtz E, Leal S, Ochatt C, Cross GA. 1999. A tightly regulated inducible expression system for conditional gene knock-outs and dominantnegative genetics in Trypanosoma brucei. Mol. Biochem. Parasitol. 99: 89-101.
24. Aslett M, Aurrecoechea C, Berriman M, Brestelli J, Brunk BP, Carrington M, Depledge DP, Fischer S, Gajria B, Gao X, Gardner MJ, Gingle A, Grant G, Harb OS, Heiges M, Hertz-Fowler C, Houston R, Innamorato F, Iodice J, Kissinger JC, Kraemer E, Li W, Logan FJ, Miller JA, Mitra S, Myler PJ, Nayak V, Pennington C, Phan I, Pinney DF, Ramasamy G, Rogers MB, Roos DS, Ross C, Sivam D, Smith DF, Srinivasamoorthy G, Stoeckert CJ, Subramanian S, Thibodeau R, Tivey A, Treatman C, Velarde G, Wang H. 2010. TriTrypDB: a functional genomic resource for the Trypanosomatidae. Nucleic Acids Res. 38: D457-D462. doi:10.1093/nar/gkp851.
25. Alsford S, Turner DJ, Obado SO, Sanchez-Flores A, Glover L, Berriman M, Hertz-Fowler C, Horn D. 2011. High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome. Genome Res. 21:915-924. doi:10.1101/gr.115089.110.
26. Absalon S, Blisnick T, Kohl L, Toutirais G, Dore G, Julkowska D, Tavenet A, Bastin P. 2008. Intraflagellar transport and functional analysis of genes required for flagellum formation in trypanosomes. Mol. Biol. Cell 19:929-944. doi:10.1091/mbc.E07-08-0749.
27. Bonhivers M, Nowacki S, Landrein N, Robinson DR. 2008. Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated. PLoS Biol. 6:e105. doi:10.1371/journal.pbio.0060105.
28. Portman N, Lacomble S, Thomas B, McKean PG, Gull K. 2009. Combining RNA interference mutants and comparative proteomics to identify protein components and dependences in a eukaryotic flagellum. J. Biol. Chem. 284:5610-5619. doi:10.1074/jbc.M808859200.
29. Vaughan S, Kohl L, Ngai I, Wheeler RJ, Gull K. 2008. A repetitive protein essential for the flagellum attachment zone filament structure and function in Trypanosoma brucei. Protist 159:127-136. doi:10.1016/j.protis.2007.08.005.
30. Cronan JE. 2005. Targeted and proximity-dependent promiscuous protein biotinylation by a mutant Escherichia coli biotin protein ligase. J. Nutr. Biochem. 16:416-418. doi:10.1016/j.jnutbio.2005.03.017.