Label-free imaging to study phenotypic behavioural traits of cells in complex co-cultures

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Suman, Rakesh ^a,b   Smith, Gabrielle ^b   Hazel, Kathryn E A ^b   Kasprowicz, Richard ^a   Coles, Mark ^b,c   O'Toole, Peter ^b   Chawla, Sangeeta ^b  

^a Phasefocus Ltd   (United Kingdom)

^b UNIVERSITY OF YORK   (United Kingdom)

^c Centre for Immunology and Infection   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; COCULTURE; MICROGLIA; NERVE CELL; PROCEDURES; RAT; TIME LAPSE IMAGING;

ANIMALS; COCULTURE TECHNIQUES; MICROGLIA; NEURONS; RATS; TIME-LAPSE IMAGING;

EID: 84959328480     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep22032     Document Type: Article

References (21)

1. Song, Y. et al. Stem cell traits in long-term co-culture revealed by time-lapse imaging. Leukemia 24, 153-161 (2009).
2. Dixit, R. & Cyr, R. Cell damage and reactive oxygen species production induced by fluorescence microscopy: effect on mitosis and guidelines for non-invasive fluorescence microscopy. Plant. J. 36, 280-290 (2003).
3. Maiden, A. M. & Rodenburg, J. M. An improved ptychographical phase retrieval algorithm for diffractive imaging. Ultramicroscopy 109, 1256-1262 (2009).
4. Kemper, B. et al. Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy. J. Biom. Opt. 15, 036009-036009-6 (2010).
5. Greenbaum, A. et al. Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy. Nat. Meth. 9, 889-895 (2012).
6. Marrison, J., Raty, L., Marriott, P. & O'Toole, P. Ptychography - a label free, high-contrast imaging technique for live cells using quantitative phase information. Sci. Rep. 3, 2369 (2013).
7. Godden, T. M., Suman, R., Humphry, M. J., Rodenburg, J. M. & Maiden, A. M. Ptychographic microscope for three-dimensional imaging. Opt. Express 22, 12513-12523 (2014).
8. Mir, M. et al. Label-Free Characterization of Emerging Human Neuronal Networks. Sci. Rep. 4 (2014).
9. Roberts, A. C. et al. Aberrant phenotype in human endothelial cells of diabetic origin: implications for saphenous vein graft failure? J. Diabetes. Res. 2015, 409432 (2015).
10. Belfield, J. L., Whittaker, C., Cader, M. Z. & Chawla, S. Differential Effects of Ca2+ and cAMP on Transcription Mediated by MEF2D and cAMP-response Element-binding Protein in Hippocampal Neurons. J. Biol. Chem. 281, 27724-27732 (2006).
11. Nimmerjahn, A., Kirchhoff, F. & Helmchen, F. Resting Microglial Cells Are Highly Dynamic Surveillants of Brain Parenchyma in Vivo. Science 308, 1314-1318 (2005).
12. Paolicelli, R. C. et al. Synaptic Pruning by Microglia Is Necessary for Normal Brain Development. Science 333, 1456-1458 (2011).
13. Hanisch, U.-K. & Kettenmann, H. Microglia: active sensor and versatile effector cells in the normal and pathologic brain. Nat. Neurosci. 10, 1387-1394 (2007).
14. Figuera-Losada, M., Rojas, C. & Slusher, B. S. Inhibition of Microglia Activation as A Phenotypic Assay in Early Drug Discovery. J. Biomol. Screen. 19, 17-31 (2014).
15. Harrison, J. K. et al. Role for neuronally derived fractalkine in mediating interactions between neurons and CX3CR1-expressing microglia. Proc. Natl. Acad. Sci. USA 95, 10896-10901 (1998).
16. Pascual, O., Ben Achour, S., Rostaing, P., Triller, A. & Bessis, A. Microglia activation triggers astrocyte-mediated modulation of excitatory neurotransmission. Proc. Natl. Acad. Sci. USA 109, E197-E205 (2012).
17. Ji, K., Akgul, G., Wollmuth, L. P. & Tsirka, S. E. Microglia Actively Regulate the Number of Functional Synapses. PLoS ONE 8, e56293 (2013).
18. Gabrusiewicz, K. et al. Characteristics of the Alternative Phenotype of Microglia/Macrophages and its Modulation in Experimental Gliomas. PLoS ONE 6, e23902 (2011).
19. Tribouillard-Tanvier, D., Carroll, J. A., Moore, R. A., Striebel, J. F. & Chesebro, B. Role of Cyclophilin A from Brains of Prion-infected Mice in Stimulation of Cytokine Release by Microglia and Astroglia in Vitro. J. Biol. Chem. 287, 4628-4639 (2012).
20. Spiller, D. G., Wood, C. D., Rand, D. A. & White, M. R. H. Measurement of single-cell dynamics. Nature 465, 736-745 (2010).
21. Huang, S. Non-genetic heterogeneity of cells in development: more than just noise. Development 136, 3853-3862 (2009).