Assessment of the Intestinal Barrier with Five Different Permeability Tests in Healthy C57BL/6J and BALB/cJ Mice

Digestive Diseases and Sciences

Volumn 61, Issue 3, 2016, Pages 737-746

  Volynets, Valentina ^a   Reichold, Astrid ^a   Bárdos, Gyöngyi ^a   Rings, Andreas ^a   Bleich, André ^b   Bischoff, Stephan C ^a  

^a UNIVERSITY OF HOHENHEIM   (Germany)

^b HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

FITC dextran; Intestinal permeability; Lactulose; Lipopolysaccharide; Ovalbumin; Polyethylene glycol

Indexed keywords

FLUORESCEIN ISOTHIOCYANATE DEXTRAN; LACTULOSE; LIPOPOLYSACCHARIDE; MACROGOL; OVALBUMIN; DEXTRAN; FLUORESCEIN ISOTHIOCYANATE; MACROGOL DERIVATIVE; MANNITOL;

ANIMAL EXPERIMENT; ARTICLE; DRUG BLOOD LEVEL; FEMALE; GENETIC BACKGROUND; IN VIVO STUDY; INTESTINE MUCOSA PERMEABILITY; MALE; MOUSE; NONHUMAN; PERMEABILITY BARRIER; PORTAL VEIN; PRIORITY JOURNAL; SEX DIFFERENTIATION; URINE; ANALOGS AND DERIVATIVES; ANIMAL; BAGG ALBINO MOUSE; BLOOD; C57BL MOUSE; INTESTINE MUCOSA; METABOLISM; PERMEABILITY;

ANIMALS; DEXTRANS; FEMALE; FLUORESCEIN-5-ISOTHIOCYANATE; INTESTINAL MUCOSA; LACTULOSE; LIPOPOLYSACCHARIDES; MALE; MANNITOL; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; OVALBUMIN; PERMEABILITY; POLYETHYLENE GLYCOLS; PORTAL VEIN;

EID: 84958907752     PISSN: 01632116     EISSN: 15732568     Source Type: Journal    
DOI: 10.1007/s10620-015-3935-y     Document Type: Article

References (24)

1. Turner JR. Intestinal mucosal barrier function in health and disease. Nat Rev Immunol. 2009;9:799–809.
2. Camilleri M, Madsen K, Spiller R, Greenwood-Van Meerveld B, Verne GN. Intestinal barrier function in health and gastrointestinal disease. Neurogastroenterol Motil. 2012;24:503–512.
3. Pastorelli L, De Salvo C, Mercado JR, Vecchi M, Pizarro TT. Central role of the gut epithelial barrier in the pathogenesis of chronic intestinal inflammation: lessons learned from animal models and human genetics. Front Immunol. 2013;4:280.
4. Bischoff SC, Barbara G, Buurman W, et al. Intestinal permeability—a new target for disease prevention and therapy. BMC Gastroenterol. 2014;14:189.
5. Grootjans J, Thuijls G, Verdam F, Derikx JP, Lenaerts K, Buurman WA. Non-invasive assessment of barrier integrity and function of the human gut. World J Gastrointest Surg. 2010;2:61–69.
6. Wang L, Llorente C, Hartmann P, Yang AM, Chen P, Schnabl B. Methods to determine intestinal permeability and bacterial translocation during liver disease. J Immunol Methods. 2015;S0022–1759:00005–00008.
7. Nejdfors P, Ekelund M, Jeppsson B, Weström BR. Mucosal in vitro permeability in the intestinal tract of the pig, the rat, and man: species- and region-related differences. Scand J Gastroenterol. 2000;35:501–507.
8. Cani PD, Rodrigo B, Knauf C, et al. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes. 2008;57:1470–1481.
9. Uhnoo IS, Freihorst J, Riepenhoff-Talty M, Fisher JE, Ogra PL. Effect of rotavirus infection and malnutrition on uptake of a dietary antigen in the intestine. Pediatr Res. 1990;27:153–160.
10. Yang Z, Chen A, Sun H, Ye Y, Fang W. Ginsenoside Rd elicits Th1 and Th2 immune responses to ovalbumin in mice. Vaccine. 2007;25:161–169.
11. Parlesak A, Schafer C, Schutz T, Bode JC, Bode C. Increased intestinal permeability to macromolecules and endotoxemia in patients with chronic alcohol abuse in different stages of alcohol-induced liver disease. J Hepatol. 2000;32:742–747.
12. Kerckhoffs AP, Akkermans LM, de Smet MB, et al. Intestinal permeability in irritable bowelsyndrome patients: effects of NSAIDs. Dig Dis Sci. 2010;55:716–723.
13. van Wijck K, Bessems BA, van Eijk HM, Buurman WA, Dejong CH, Lenaerts K. Polyethylene glycol versus dual sugar assay for gastrointestinal permeability analysis: Is it time to choose? Clin Exp Gastroenterol. 2012;5:139–150.
14. Braga Neto MB, Aguiar CV, Maciel JG, et al. Evaluation of HIV protease and nucleoside reverse transcriptase inhibitors on proliferation, necrosis, apoptosis in intestinal epithelial cells and electrolyte and water transport and epithelial barrier function in mice. BMC Gastroenterol. 2010;10:90.
15. Vilela EG, Torres HO, Ferrari ML, Lima AS, Cunha AS. Gut permeability to lactulose and mannitol differs in treated Crohn’s disease and celiac disease patients and healthy subjects. Braz J Med Biol Res. 2008;41:1105–1109.
16. Kubica P, Kot-Wasik A, Wasik A, Namieśnik J, Landowski P. Modern approach for determination of lactulose, mannitol and sucrose in human urine using HPLC-MS/MS for the studies of intestinal and upper digestive tract permeability. J Chromatogr B Analyt Technol Biomed Life Sci. 2012;907:34–40.
17. Ritze Y, Bárdos G, D’Haese JG, et al. Effect of high sugar intake on glucose transporter and weight regulating hormones in mice and humans. PLoS One. 2014;9:e101702.
18. Chang JX, Chen S, Ma LP, et al. Functional and morphological changes of the gut barrier during the restitution process after hemorrhagic shock. World J Gastroenterol. 2005;11:5485–5491.
19. Giorgio V, Miele L, Principessa L, et al. Intestinal permeability is increased in children with non-alcoholic fatty liver disease, and correlates with liver disease severity. Dig Liver Dis. 2014;46:556–560.
20. Kless C, Müller VM, Schüppel VL, et al. Diet-induced obesity causes metabolic impairment independent of alterations in gut barrier integrity. Mol Nutr Food Res. 2015;59:968–978.
21. Yokooji T, Nouma H, Matsuo H. Characterization of ovalbumin absorption pathways in the rat intestine, including the effects of aspirin. Biol Pharm Bull. 2014;37:1359–1365.
22. Meddings JB, Jarand J, Urbanski SJ, Hardin J, Gall DG. Increased gastrointestinal permeability is an early lesion in the spontaneously diabetic BB rat. Am J Physiol. 1999;276:G951–G957.
23. Anderson AD, Jain PK, Fleming S, Poon P, Mitchell CJ, MacFie J. Evaluation of a triple sugar test of colonic permeability in humans. Acta Physiol Scand. 2004;182:171–177.
24. Meddings JB, Gibbons I. Discrimination of site-specific alterations in gastrointestinal permeability in the rat. Gastroenterology. 1998;114:83–92.