Young investigator challenge: Validation and optimization of immunohistochemistry protocols for use on cellient cell block specimens

Cancer Cytopathology

Volumn 124, Issue 2, 2016, Pages 89-99

  Sauter, Jennifer L ^a   Grogg, Karen L ^a   Vrana, Julie A ^a   Law, Mark E ^a   Halvorson, Jennifer L ^a   Henry, Michael R ^a  

^a MAYO CLINIC   (United States)

Author keywords

antibodies; cell block; Cellient; exfoliative cytology; immunohistochemistry; validation

Indexed keywords

CALRETININ; CD20 ANTIGEN; CD3 ANTIGEN; CYTOKERATIN 20; ESTROGEN RECEPTOR; PROTEIN ANTIBODY; PROTEIN P16; TRANSCRIPTION FACTOR CDX2; ANTIBODY;

ARTICLE; BODY FLUID; CELLIENT CELL BLOCK; CONTROLLED STUDY; CYTOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; LYMPH NODE; PLEURA FLUID; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS; PROCESS OPTIMIZATION; SMALL CELL CARCINOMA; SQUAMOUS CELL CARCINOMA; THROMBIN CELL BLOCK; TRANSBRONCHIAL ASPIRATION; VALIDATION PROCESS; COMPARATIVE STUDY; CYTODIAGNOSIS; FEMALE; HISTOLOGY; IMMUNOLOGY; LABORATORY DIAGNOSIS; MALE; NEOPLASM; PATHOLOGY; PERSONNEL; PROCEDURES; SENSITIVITY AND SPECIFICITY; TISSUE FIXATION; VALIDATION STUDY;

ANTIBODIES; CYTODIAGNOSIS; FEMALE; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; NEOPLASMS; RESEARCH PERSONNEL; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; TISSUE EMBEDDING; TISSUE FIXATION;

EID: 84958793819     PISSN: 1934662X     EISSN: 19346638     Source Type: Journal    
DOI: 10.1002/cncy.21660     Document Type: Article

References (24)

1. Hologic Inc. Cellient home page. http://www.cellientsystem.com. Accessed September 18, 2015.
2. Prendeville S, Brosnan T, Browne TJ, McCarthy J,. Automated Cellient(™) cytoblocks: better, stronger, faster? Cytopathology. 2014; 25: 372-380.
3. Jain D, Mathur SR, Iyer VK,. Cell blocks in cytopathology: a review of preparative methods, utility in diagnosis and role in ancillary studies. Cytopathology. 2014; 25: 356-371.
4. Hologic Inc. The ThinPrep 2000 System [package insert]. Marlborough, MA: Hologic Inc; 2010.
5. Fitzgibbons PL, Bradley LA, Fatheree LA, et al,; College of American Pathologists Pathology and Laboratory Quality Center. Principles of analytic validation of immunohistochemical assays: guideline from the College of American Pathologists Pathology and Laboratory Quality Center. Arch Pathol Lab Med. 2014; 138: 1432-1443.
6. Cytyc Corporation. Cytyc to introduce Cellient™ automated cell block system at American Society of Cytopathology Annual Meeting [press release]. http://www.sec.gov/Archives/edgar/data/849778/000119312506216836/dex991.htm. Accessed September 18, 2015.
7. Ventana. OptiView detection chemistry. http://www.ventana.com/documents/OptiView-F&B-Brochure.pdf. Accessed September 18, 2015.
8. Bramwell NH, Burns BF,. The effects of fixative type and fixation time on the quantity and quality of extractable DNA for hybridization studies on lymphoid tissue. Exp Hematol. 1988; 16: 730-732.
9. Srinivasan M, Sedmak D, Jewell S,. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol. 2002; 161: 1961-1971.
10. van Hemel BM, Suurmeijer AH,. Effective application of the methanol-based PreservCyt(™) fixative and the Cellient(™) automated cell block processor to diagnostic cytopathology, immunocytochemistry, and molecular biology. Diagn Cytopathol. 2013; 41: 734-741.
11. Dejmek A, Zendehrokh N, Tomaszewska, Edsjo A,. Preparation of DNA from cytological material: effects of fixation, staining, and mounting medium on DNA yield and quality. Cancer (Cancer Cytopathol). 2013; 121: 344-353.
12. Sauter JL, Ambaye AB, Mount SL,. Increased utilization, verification, and clinical implications of immunocytochemistry: experience in a northern New England hospital. Diagn Cytopathol. 2015; 43: 688-695.
13. Gruchy JR, Barnes PJ, Dakin Hache KA,. CytoLyt® fixation and decalcification pretreatments alter antigenicity in normal tissues compared with standard formalin fixation. Appl Immunohistochem Mol Morphol. 2015; 23: 297-302.
14. Konno F, Jungbluth A, Frosina D, et al., Drastic loss of MIB1/Ki67 immunoreactivity in CytoLyt-fixed cell blocks [abstract]. Mod Pathol. 2015; 28 (suppl 2): 95A.
15. Kruger AM, Stevens MW, Kerley KJ, Carter CD,. Comparison of the Cellient(™) automated cell block system and agar cell block method. Cytopathology. 2014; 25: 381-388.
16. Gorman BK, Kosarac O, Chakraborty S, Schwartz MR, Mody DR,. Comparison of breast carcinoma prognostic/predictive biomarkers on cell blocks obtained by various methods: Cellient, formalin and thrombin. Acta Cytol. 2012; 56: 289-296.
17. Wagner DG, Russell DK, Benson JM, Schneider AE, Hoda RS, Bonfiglio TA,. Cellient™ automated cell block versus traditional cell block preparation: a comparison of morphologic features and immunohistochemical staining. Diagn Cytopathol. 2011; 39: 730-736.
18. Hanley KZ, Birdsong GG, Cohen C, Siddiqui MT,. Immunohistochemical detection of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 expression in breast carcinomas: comparison on cell block, needle-core, and tissue block preparations. Cancer (Cancer Cytopathol). 2009; 117: 279-288.
19. Williams SL, Birdsong GG, Cohen C, Siddiqui MT,. Immunohistochemical detection of estrogen and progesterone receptor and HER2 expression in breast carcinomas: comparison of cell block and tissue block preparations. Int J Clin Exp Pathol. 2009; 2: 476-480.
20. Gong Y, Symmans WF, Krishnamurthy S, Patel S, Sneige N,. Optimal fixation conditions for immunocytochemical analysis of estrogen receptor in cytologic specimens of breast carcinoma. Cancer (Cancer Cytopathol). 2004; 102: 34-40.
21. Jing X, Li QK, Bedrossian U, Michael CW,. Morphologic and immunocytochemical performances of effusion cell blocks prepared using 3 different methods. Am J Clin Pathol. 2013; 139: 177-182.
22. Kinsella MD, Birdsong GG, Siddiqui MT, Cohen C, Hanley KZ,. Immunohistochemical detection of estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2 in formalin-fixed breast carcinoma cell block preparations: correlation of results to corresponding tissue block (needle core and excision) samples. Diagn Cytopathol. 2013; 41: 192-198.
23. Hardy LB, Fitzgibbons PL, Goldsmith JD, et al., Immunohistochemistry validation procedures and practices: a College of American Pathologists survey of 727 laboratories. Arch Pathol Lab Med. 2013; 137: 19-25.
24. Fischer AH, Schwartz MR, Moriarty AT, et al., Immunohistochemistry practices of cytopathology laboratories: a survey of participants in the College of American Pathologists Nongynecologic Cytopathology Education Program. Arch Pathol Lab Med. 2014; 138: 1167-1172.