Immunohistochemical detection of estrogen and progesterone receptor and HER2 expression in breast carcinomas: Comparison of cell block and tissue block preparations

International Journal of Clinical and Experimental Pathology

Volumn 2, Issue 5, 2009, Pages 476-480

  Williams, Sharenda L ^a   Birdsong, George G ^b   Cohen, Cynthia ^a   Siddiqui, Momin T ^a  

^a EMORY UNIVERSITY HOSPITAL   (United States)

^b GRADY MEMORIAL HOSPITAL   (United States)

Author keywords

Alcohol fixation; Breast carcinoma; Cell block; ER; Fine needle aspiration; Formalin fixation; HER2; Immunohistochemistry; PR

Indexed keywords

EID: 67149113237     PISSN: None     EISSN: 19362625     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Dowsett M, Hanna WM, Kockx M, Penault-Llorca F, Ruschoff J, Gutjahr T, Habben K and van de Vijver M. Standardization of HER2 testing: results of an international proficiency-testing ring study. Mod Pathol 2007;20:584-591.
2. Gown AM. Current issues in ER and HER2 testing by IHC in breast cancer. Mod Pathol 2008;21:S8-S15.
3. Diaz NM. Laboratory testing for HER2/neu in breast carcinoma: An evolving strategy to predict response to targeted therapy. Cancer Control 2001;8:415-418.
4. Fitzgibbons PL, Murphy DA, Dorfman DM, Roche PC and Tubbs R. Interlaboratory comparison of immunohistochemical testing for HER2 - Results of the 2004 and 2005 College of American Pathologists HER2 imunohistochemistry tissue microarray survey. Arch Pathol Lab Med 2006;130:1440-1445.
5. Jacobs TW, Gown AM, Yaziji H, Barnes MJ and Schnitt SJ. Specificity of HercepTest in determining HER-2/neu status of breast cancers using the United States Food and Drug Administration-approved scoring system. J Clin Oncol 1999;17:1983-1987.
6. Wolff AC, Hammond EH, Schwartz JN, Hagerty KL, Allred DC, Cote RJ, Dowsett M, Fitzgibbons PL, Hanna WM, Langer A, McShane LM, Paik S, Pegram MD, Perez EA, Press MF, Rhodes A, Sturgeon C, Taube S, Tubbs R, Vance G, van de Vijver M, Wheeler TM and Hayes DF. American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. Arch Pathol Lab Med 2007;131:18-43.
7. Rhodes A, Borthwick D, Sykes R, Al-Sam S and Paradiso A. The use of cell line standards to reduce HER-2/neu assay variation in multiple European cancer centers and the potential of automated image analysis to provide for more accurate cut points for predicting clinical response to trastuzumab. Am J Clin Pathol 2004;122:51-60.
8. Gown AM and Yaziji H. Accuracy and precision in HER2/neu testing in breast cancer: Are we there yet? Hum Pathol 2004;35:143-146.
9. Bilous MA, Dowsett M, Hanna W, Isola J, Lebeau A, Moreno A, Penault-Llorca F, Ruschoff J, Tomasic G and van de Vijver M. Current perspectives on HER2 testing: A review of national testing guidelines. Mod Pathol 2003; 16:173-182.
10. Zarbo RJ and Hammond EM. Conference summary, strategic science symposium: Her2-/neu testing of breast cancer patients in clinical practice. Arch Pathol Lab Med 2003; 127:549-553.
11. Vani K, Sompuram SR, Fitzgibbons P and Bogen SA. National HER2 proficiency test results using standardized quantitative controls. Arch Pathol Lab Med 2008;132:211-216.
12. Cell Markers and Cytogenetic Committees, College of American Pathologists. Clinical laboratory assays for HER-2/neu amplification and overexpression - quality assurance, standardization, and proficiency testing. Arch Pathol Lab Med 2002;126:803-808.
13. Goldstein NS, Ferkowicz M, Odish E, Mani A and Hastah F. Minimum formalin fixation time for consistent estrogen receptor immunohistochemical staining of invasive breast carcinoma. Am J Clin Pathol 2003;120: 86-92.
14. Werner M, Chott A, Fabiano A and Battifora H. Effect of formalin tissue fixation and processing on immunohistochemistry. Am J Surg Pathol 2000;24:1016-1019.
15. Dabbs DJ. Immunohistochemical protocols. Am J Clin Pathol 2008;129:355-356.
16. Nassiri M, Ramos S, Zohourian H, Vincek V, Morales AR and Nadji M. Preservation of biomolecules in breast cancer tissue by a formalin-free histology system. BMC Clin Pathol 2008;8:1-10.
17. Folkvord JM, Viders D, Coleman-Smith A and Clark RAF. Optimization of immunohistochemical techniques to detect extracellular matrix proteins in fixed skin specimens. J Histochem Cytochem 1989;37: 105-113.
18. Fox C, Johnson FB, Whiting J and Roller PP. Formaldehyde fixation. J Histochem Cytochem 1985;33:845-853.