Detection of culturable and viable but non-culturable cells of beer spoilage lactic acid bacteria by combined use of propidium monoazide and horA-specific polymerase chain reaction

Journal of the Institute of Brewing

Volumn 122, Issue 1, 2016, Pages 29-33

  Deng, Yang ^a,b,c   Zhao, Junfeng ^d   Li, Huiping ^c   Xu, Zhenbo ^b   Liu, Junyan ^b   Tu, Jingxia ^c   Xiong, Tao ^a  

^a NANCHANG UNIVERSITY   (China)

^b SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^c Guangzhou Zhujiang Brewery Co Ltd   (China)

^d HENAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Beer spoilage lactic acid bacteria; Hop resistance gene; Polymerase chain reaction; Propidium monoazide

Indexed keywords

BACTERIA; BEER; CYTOLOGY; GENES; LABORATORIES; LACTIC ACID; POLYMERS; SPOILAGE;

HIGHLY-CORRELATED; HOP RESISTANCES; LACTIC ACID BACTERIA; PCR AMPLIFICATION; PROPIDIUM; REFERENCE STRAINS; SUBSTANTIAL REDUCTION; VIABLE BUT NON-CULTURABLE;

POLYMERASE CHAIN REACTION;

EID: 84958749137     PISSN: 00469750     EISSN: 20500416     Source Type: Journal    
DOI: 10.1002/jib.289     Document Type: Article

References (19)

1. Sakamoto, K., and Konings, W. N. (2003) Beer spoilage bacteria and hop resistance, Int. J. Food Microbiol. 89, 105-124.
2. Suzuki, K., Iijima, K., Sakamoto, K., Sami, M., and Yamashita, H. (2006) A review of hop resistance in beer spoilage lactic acid bacteria, J. Inst. Brew. 112, 173-191.
3. Deng, Y., Liu, J., Li, H., Li, L., Tu, J., Fang, H., Chen, J., and Qian, F. (2014) An improved plate culture procedure for the rapid detection of beer spoilage lactic acid bacteria, J. Inst. Brew. 120, 127-132.
4. Deng, Y., Liu, J., Li, L., Fang, H., Tu, J., Li, B., Liu, J., Li, H., and Xu, Z. (2015) Reduction and restoration of culturability of beer-stressed and low-temperature-stressed Lactobacillus acetotolerans strain 2011-8, Int. J. Food Microbiol. 206, 96-101.
5. Suzuki, K., Iijima, K., Asano, S., Kuriyama, H., and Kitagawa, Y. (2006) Induction of viable but nonculturable state in beer spoilage lactic acid bacteria, J. Inst. Brew. 112, 295-301.
6. Haakensen, M., Schubert, A., and Ziola, B. (2008) Multiplex PCR for putative Lactobacillus and Pediococcus beer spoilage genes and ability of gene presence to predict growth in beer, J. Am. Soc. Brew. Chem. 66, 63-70.
7. Pfannbecker, J., and Fröhlich, J. (2008) Use of a species-specific multiplex PCR for the identification of pediococci, Int. J. Food Microbiol. 128, 288-296.
8. Pawlowsky, K., Walker, S., Hammond, J., and Brandl, A. (2006) A novel PCR method for the detection of live microorganisms, Bri. Rev. 7, 6-10.
9. Seinige, D., Krischek, C., Klein, G., and Kehrenberg, C. (2014) Comparative analysis and limitations of ethidium monoazide and propidium monoazide treatments for the differentiation of viable and nonviable Campylobacter cells, Appl. Environ. Microbiol. 80, 2186-2192.
10. Nocker, A., Sossa, K. E., and Camper, A. K. (2007) Molecular monitoring of disinfection efficacy using propidium monoazide in combination with quantitative PCR, J. Microbiol. Meth. 70, 252-260.
11. Suzuki, K., Sami, M., Iijima, K., Ozaki, K., and Yamashita, H. (2006) Characterization of horA and its flanking regions of Pediococcus damnosus ABBC478 and development of more specific and sensitive horA PCR method, Lett. Appl. Microbiol. 42, 392-399.
12. Iijima, K., Suzuki, K., Asano, S., Kuriyama, H., and Kitagawa, Y. (2007) Isolation and identification of potential beer spoilage Pediococcus inopinatus and beer spoilage Lactobacillus backi strains carrying the horA and horC gene clusters, J. Inst. Brew. 113, 96-101.
13. Menz, G., Andrighetto, C., Lombardi, A., Corich, V., Aldred, P., and Vriesekoop, F. (2010) Isolation, identification, and characterisation of beer spoilage lactic acid bacteria from microbrewed beer from Victoria, Australia, J. Inst. Brew. 116, 14-22.
14. Asano, S., Suzuki, K., Iijima, K., Motoyama, H., Kuriyama, H., and Kitagawa, Y. (2007) Effects of morphological changes in beer spoilage lactic acid bacteria on membrane filtration in breweries, J. Biosci. Bioeng. 104, 334-338.
15. Oliver, J. D. (2010) Recent findings on the viable but nonculturable state in pathogenic bacteria, FEMS Microbiol. Rev. 34, 415-425.
16. Porter, J., Edwards, C., and Pickup, R. W. (1995) Rapid assessment of physiological status in Escherichia coli using fluorescent probes, J. Appl. Bacteriol. 4, 399-408.
17. Sami, M., Yamashita, H., Kadokura, H., Kitamoto, K., Yoda, K., and Yamasaki, M. (1997) A new and rapid method for determination of beer-spoilage ability of lactobacilli, J. Am. Soc. Brew. Chem. 55, 137-140.
18. Sakamoto, K., Margolles, A., van Veen, H. W., and Konings, W. N. (2001) Hop resistance in the beer spoilage bacterium Lactobacillus brevis is mediated by the ATP-binding cassette multidrug transporter HorA, J. Bacteriol. 183, 5371-5375.
19. Haakensen, M. C., Butt, L., Chaban, B., Deneer, H., Ziola, B., and Dowgiert, T. (2007) horA-specific real-time PCR for detection of beer spoilage lactic acid bacteria, J. Am. Soc. Brew. Chem. 65, 157-165.